Viabilitas Sperma Ikan Nilem (Osteochilus hasselti C.V.) Setelah Penyimpanan Jangka Pendek dalam Larutan Ringer

https://doi.org/10.22146/jfs.142

Gratiana E. Wijayanti(1*), Sorta B.I. Simanjuntak(2)

(1) Lab. Struktur dan Perkembangan Hewan Fakultas Biologi UNSOED
(2) Lab Fisiologi Hewan Fakultas Biologi UNSOED
(*) Corresponding Author

Abstract


The success of induced breeding relies on the availability of viable sperm and eggs. Meanwhile information on viability of post-stripped sperm of the nilem is still limited.  This experimental study was conducted to find out the duration of sperm storage that maintains sperm viability.  The nilem’s milt was diluted in Ringer solution at 0, 10, 100 and 1,000 dilution and kept in the refrigerator (4°C)  for 3, 6 and 9 days.  The sperm viability was
assessed by its motility, progressivity and ability to fertilize fresh eggs.  The results showed that the sperm were immotile in either undiluted or diluted in Ringer solution.  Upon activation by diluting in water, the sperm of the control groups as well as the tested groups showed a high motility (>70%) and highly progressive (>3).  The sperm were capable to fertilize eggs as indicated by a high fertilization rate of more than 90%.  The highest hatching rate (HR) was produced by the control groups while HR of milt stored for 3, 6 and 9 days were 60%, 1% and 0%, respectively. Low HR produced by milt stored for 6 and 9 days was resulted from a high proportion of abnormal embryos which led to their mortality around gastrula stage.  It could be concluded that the viability of the nilem’s sperm could be diluted in Ringer solution and kept in the refrigerator as long as for 3 days.

Keywords


sperm storage, sperm motility, fertility, nilem (Osteochilus hasselti C.V.)

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DOI: https://doi.org/10.22146/jfs.142

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