* Corresponding Author
Viabilitas Sperma Ikan Nilem (Osteochilus hasselti C.V.) Setelah Penyimpanan Jangka Pendek dalam Larutan Ringer
https://doi.org/10.22146/jfs.142 Gratiana E. Wijayanti(1*), Sorta B.I. Simanjuntak(2)
(1) Lab. Struktur dan Perkembangan Hewan Fakultas Biologi UNSOED
(2) Lab Fisiologi Hewan Fakultas Biologi UNSOED
(*) Corresponding Author
Abstract
assessed by its motility, progressivity and ability to fertilize fresh eggs. The results showed that the sperm were immotile in either undiluted or diluted in Ringer solution. Upon activation by diluting in water, the sperm of the control groups as well as the tested groups showed a high motility (>70%) and highly progressive (>3). The sperm were capable to fertilize eggs as indicated by a high fertilization rate of more than 90%. The highest hatching rate (HR) was produced by the control groups while HR of milt stored for 3, 6 and 9 days were 60%, 1% and 0%, respectively. Low HR produced by milt stored for 6 and 9 days was resulted from a high proportion of abnormal embryos which led to their mortality around gastrula stage. It could be concluded that the viability of the nilem’s sperm could be diluted in Ringer solution and kept in the refrigerator as long as for 3 days.
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Alvi, S.M.H., J. Cosson, M. Karami, B.M. Amiri, and M.A. Akhoundzadeh. 2004. Spermatozoa motility in the Persian sturgeon, Acipenser persicus: effect of pH, dilution rate, ions and osmolality. Reproduction. 128: 819-828.
Billard, R. 1978. Chanes in structure and fertilizing ability of marine and freshwater fish spermatozoa diluted in media of various salinities. Aquaculture. 14: 187- 198.
Billard, R. 1986. Spermatogenesis and spermatology of some teleost fish species. Reproduction, Nature and Development. 2: 877-920.
Billard, R. and M.P. Cosson. 1992. Some problems related to the assessment of sperm motility in freshwater fish. J. Experimental Zoology. 261: 122-131.
Billard, R., J. Cosson, S.B. Noveiri, and M. Pourkazemi. 2004. Cryopreservation and short-term storage of sturgeon sperm, a review. Aquaculture. 236: 1-9.
Cosson, J., R. Billard, C. Cibert, C. Dreanno, and M. Suquet. 1999. Ionic factor regulating the motility of fish sperm. In: The male gamete: from basic science to clinical applications. C. Gagnon (Ed). Cache River Press. Vienna. 162-186.
DiLauro, M.N., W.F. Krise, M.A. Hendrix, and S.E. Baker. 1994. Short-term cold storage of atlantic sturgeon sperm. Progressive Fish- Culturist. 56: 143-144.
Ingermann, R.L., M. Holcomb, M.L. Robinson, and J.G. Cloud. 2002. Carbondioxide and pH affect sperm motility of white sturgeon (Acipenser transmontanus). J. Experimental Zoology. 205: 2885-2890.
Jezierska B. and M. Witeska. 1999. The effect of time and temperature on motility of spermatozoa of common and grass carp. Electronic Jounal of Polish Agricultural Universities, Fisheries, 2(2). http:www//ejpau.media.pl/sries/volume2/issue2/fisheries/art-04.html. Diakses tanggal 14 Juni 2005.
Kavamoto, E.T., W. Fogli da Silva, M.G. Regolino, Y.A. Tabata, and B.E.S. Campos. 2001. Producao espermatica e teste de fertilizacao do semen de truta arco-iris, Salmo irideus Gibbons no primeiro ciclo reprodutivo. B. Inst Pesca. 14: 51-62.
Kopeika, F.F., S.I. Drokin, V.V. Cherepanov, B.B. Dzuba, and L.L. Tsvetkova. 1997. Oxygen and fish spermatozoa cryoresistance. 66Cryo’97. 34th Annual Metting of the Scociety for Cryobiology 66. Barcelona, June 1997. Abstract.
Marques S. and H.P. Godinho. 2004. Short-term cold storage of sperm from six neotropical characiformes fishes. Brazilian Archives of Biology and Technology. 47(4): 799-804.
Martini, R. 1998. Viabilitas spermatozoa ikan nilem (Osteochilus hasselti C.V.) setelah diejakulasikan. Skripsi. Fakultas Biologi UNSOED. Purwokerto. 60 p.
McNiven, M.A., R.K. Gallant, and G.F. Richardson. 1993. Fresh storage of rainbow trout (Onchorynchus mykiss) semen using a nonaqueous medium. Aquaculture. 109: 71-82.
Mochida, K., T. Kondo, T. Matsubara, S. Adachi, and K. Yamauchi. 1999. A high molecular weight glycoprotein in seminal plasma is a sperm immobilizing factor in the teleost Nile tilapia, Oreochromis mossambicus. Development Growth and Differentiation 41: 619-627.
Park, C. and F.A. Chapman. 2005. An extender slution for the short-term storage of sturgeon semen. North American Journal of Aquaculture. 67: 52-57.
Sensusinawati, S.W. 1983. Pengaruh lama penyimpanan sperma ikan mas terhadap keberhasilan pembuahan dan penetasan telur. Karya Ilmiah. Fakultas Perikanan IPB, Bogor. 65 p.
Stoss, J. 1983. Fish gamet preservation and spermatozzon physiology. In: Fish Physiology IXB. W.S. Hoar, D.J. Randall, E.M. Doaldson (Eds.). Academic Press New York. 305-350.
Wijayanti, G.E., Soeminto, S.B.I. Simanjuntak, P. Susatyo, dan A.E. Pulungsari. 1995. Studi pendahuluan untuk peningkatan mutu benih ikan nilem (Osteochilus hasselti C.V.) melalui induksi dan penetasan dalam akuarium. Laporan Penelitian. Fakultas Biologi UNSOED. Purwokerto. 43 p.
Wijayanti, G.E., Sugiharto, S.B.I. Simanjuntak, P. Susatyo, dan E.T. Winarni. 1997. Fertilitas telur dan sperma ikan nilem (Osteochilus hasselti C.V.) pasca stripping dalam media alami. Laporan Penelitian. Fakultas Biologi UNSOED. Purwokerto. 41 p.
Wijayanti, G.E., Sugiharto, P. Susatyo, dan A. Nuryanto. 1998. Perkembangan embrio dan larva ikan nilem yang diinkubasi pada media dengan berbagai temperatur. Laporan Penelitian. Fakultas Biologi UNSOED. Purwokerto. 40 p.
Zamir, E., Z. Kam, and A. Yarden. 1997. Transcription-dependent induction of G1 phase during the zebra fish midblastula transsition. Molecular and Cellular Biology. 17(2): 529-536.
DOI: https://doi.org/10.22146/jfs.142
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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).
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