Pengaruh Salinitas dan Kondisi Fertilisasi terhadap Persentase Larva Normal pada Kerang Bakau Polymesoda erosa Solander (1786)

Ricky Gimin(1*)

(1) Jurusan Perikanan dan Kelautan, Universitas Nusa Cendana
(*) Corresponding Author


The mangrove clam (Polymesoda erosa) is edible bivalve and one of potential candidate for aquaculture. Availability of its hatchery technique is important for development of its aquaculture. The objective of this study was to determine the optimum salinity and fertilization factors for the production of normal larvae. Fertilized eggs at density of 10 eggs/ml were incubated under seven salinities, i.e., 0, 5, 10, 15, 20, 25 and 30 ppt at room temperature (26±1°C). The highest percentage of 48 hr normal D-larvae occurred at 20 ppt.
The percentage dropped significantly if salinity was below 10 ppt or more than 25 ppt. In other experiments to develop fertilization protocols for this species, the effects of spermatozoa-to-ovocyte ratio, age of gametes, and stocking density of eggs were assessed individually to determine the optimum ratio, age and density for the highest percentage of normal D-larvae. Ten sperms-to-egg ratios, i.e., 5, 10, 50, 102, 5x102, 103, 5x103, 104, 5x104 and 105 at density of 10 eggs/ml, were tested. The highest percentage of normal D-larvae occurred within the range of 5x102 to 5x103 sperms per egg. Significantly low percentages were obtained at treatments below 100 or more than 104 sperms per egg. In the experiment of gamete age, sperms and eggs were mixed for fertilization at different times, i.e., 30 minutes, 45 minutes, 60 minutes, 120 minutes and 180 minutes, after spawning. Freshly spawned sperms and eggs of less than 60 minutes gave the best percentage. When the fertilized eggs were incubated at different densities, i.e., 10, 20, 30, 40, 50, 100 and 200 eggs/ml, the densities up to 50 eggs/ml gave maximum results.


Polymesoda erosa, salinity, fertilization, D-larvae

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Clavier, J. 1992. Fecundity and optimal sperm density for fertilization of the ormer, Haliotis tuberculate L. In Abalones of the world: Biology, fisheries and culture. S. A. Shepherd, M. J. Tegner, S. A. Guzmán del Próo (Eds.), Fishing News Books. Oxford: 86-92.

Clotteau, G. and F. Dubé. 1993. Optimization of fertilization parameters for rearing surf clams (Spisula solidissima). Aquaculture. 114: 339-353.

Doroudi, M.S., P.C. Southgate, and R.J. Mayer. 1999. The combined effects of temperature and salinity on embryos and larvae of the black-lip pearl oyster, Pinctada margaritifera (L.). Aquaculture Research. 30: 271-277.

Dos Santos, A.E. and I.A. Nascimento. 1985. Influence of gamete density, salinity and temperature on the normal embryonic development of the mangrove oyster Crassostrea rhizophorae Guilding, 1828. Aquaculture. 47: 335-352.

Encena, V.C., E.C. Capitin Jr, and N.C. Bayona. 1998. Optimal sperm concentration and time for fertilization of the tropical abalone, Haliotis asinina Linne 1758. Aquaculture. 165: 347-352.

Gibbons, M.C. and M. Castagna. 1984. Serotonin as an inducer of spawning in six bivalve species. Aquaculture. 40: 189-191.

Gimin, R. 2002. Induction of spawning and larval rearing of the mangrove clam, Polymesoda (Geloina) erosa Solander (1786) (Bivalvia: Corbiculidae). Paper presented at Aquafest Conference-Australia 2002. 19-22 September 2002, Hobart-Tasmania.

Gimin, R. 2004. Reproduction and conditioning of the mangrove clam Polymesoda (Geloina) erosa (Bivalvia: Corbiculidae) (Solander, 1786). PhD Thesis Charles Darwin University-Australia. 213 p.

Gruffydd, L.D. and A.R. Beaumont. 1970. Determination of the optimum concentration of eggs and spermatozoa for the production of normal larvae in Pecten maximus (Mollusca, Lamellibranchia). Helgoländer Wissenschaftliche Meeresuntersuchungen. 20: 486-497.

Hahn, K.O. 1989. Handbook of culture of abalone and other marine gastropods. CRC Press, Boca Raton, FL. 348 p.

Heasman, M.P., W.A. O’Connor, and A.W. Frazer. 1996. Effects of fertilization and incubation factors on the quality and yield of scallop, Pecten fumatus Reeve, larvae. Aquaculture Research. 27: 505-513.

Helm, M.M. and P.F. Millican. 1977. Experiments in the hatchery rearing of Pacific oyster larvae (C. gigas Thunberg). Aquaculture. 11: 1-12.

His, E., R. Robert, and A. Dinet. 1989. Combined effects of temperature and salinity on fed and starved larvae of the Mediterranean mussel Mytilus galloprovincialis and the Japanese oyster Crassostrea gigas. Marine Biology. 100: 455-463.

Hurley, D.H. and R.L. Walker. 1996. The effects of larval stocking density on growth, survival, and development of laboratory-reared Spisula solidissima similis (Say, 1822). Journal of Shellfish Research. 15(3):715-718.

Loosanoff, V.L. and H.C. Davis. 1963. Rearing of bivalve mollusks. Advances in Marine Biology. 1: 1-136.

Meehan, B. 1982. Shell bed to shell midden. Australian Institute of Aboriginal Studies. Canberra. 237 p.

Morton, B. 1988. The population structure and age of Polymesoda (Geloina) erosa (Bivalvia: Corbiculacea) from a Hong Kong mangrove. Asian Marine Biology. 5: 107-113.

Narvarte, M.A. and M.S. Pascual. 2003. Fertilization, larval rearing and post-larval growth of the Tehuelche scallop Aequipecten tehuelchus D’Orb., 1846. Aquaculture. 217: 259-274.

O’Connor, W.A. and M.P. Heasman. 1995. Spawning induction and fertilization in the doughboy scallop Chlamys (Mimachlamys) asperrima. Aquaculture. 136: 117-129.

Pennington, J.T. 1985. The ecology of fertilization of echinoid eggs: The consequences of sperm dilution, adult aggregation, and synchronous spawning. Biological Bulletin. 169: 417 430.

Stephano, J.L. and M. Gould. 1988 Avoiding polyspermae in the oyster (Crassostrea gigas). Aquaculture. 73: 295-307.


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Jurnal Perikanan Universitas Gadjah Mada (print ISSN 0853-6384; online ISSN 2502-5066) is published by Department of Fisheries, Universitas Gadjah Mada in collaboration with Semnaskan UGM (Seminar Nasional Tahunan Hasil Perikanan dan Kelautan) and ISMFR (International Symposium on Marine and Fisheries Research).


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