Antibacterial Analysis of the Ethanol Extract of Gonggong Snail (Strombus canarium) against Pathogenic Bacteria

https://doi.org/10.22146/jfs.10346

Dessy Yoswaty(1*), Zulkifli Zulkifli(2)

(1) 
(2) 
(*) Corresponding Author

Abstract


The study aims to analyze the use of antibacterial ethanol extract of gonggong snail (Strombus canarium) against pathogenic bacteria and analyzing bioactive compounds in gonggong snails for the development of marine aquaculture. The research method is an experimental method, performed in vitro, using a completely
randomized design (CRD) with three replications. The subjects of research that pathogenic bacteria (Vibrio sp., C. perfringens, and Aeromonas sp.). The object of research is D1 gonggong snail extract 12.5%; D2 gongong snail extract 25%; D3 gonggong snail extract 50%; C4 gonggong snail extract 100%, ethanol D5 and D6 amosiklav discs (antibiotic chloramphenicol). Phytochemical screening test that alkaloids, saponins, flavonoids, steroids, and triterpenoids) from the ethanol extract of the gonggong snail. Data were analyzed by ANOVA test to determine the significance of the difference in diameter between the various treatments inhibitory region. The results showed that as many as 20 of the gonggong snail with morphometric body length size range of between 43.9 to 56.0 mm, the average length of 52.74 mm and a total weight of 16.21 grams on average. Results of research ethanol extract of gonggong snail is treated discs amoxiclav have inhibition (clear zone) highs against the bacterium Vibrio sp. (diameter 1.80 to 12.40 mm), A. hydrophila (diameter 4.78 to 21.37 mm) and C. perfringens (diameter 1.50 to 6.80 mm). Class of bioactive compounds of alkaloids and saponins identified in the ethanol extract of gonggong snail and class of bioactive compounds of flavanoid, steroids, and triterpenoids is not identified in the ethanol extract of gonggong snails. The results of the measurement of environmental quality parameters in waters around the coast of Bintan Island Village Senggarang still in good condition and has not been contaminated. This enables to support life gonggong snail (S. canarium). The ethanol extract of gonggong snails has the antibacterial activity that can be used to overcome the attacks of pathogenic bacteria in the development of marine aquaculture.


Keywords


antibacterial, bioactive compounds, gonggong snail

Full Text:

PDF


References

Andiarto, H. 2011 Studi ekologi, morfometri tedong gonggong (Strombus canarium Linne 1758) dan asosiasinya dengan fauna moluska di perairan Pulau Bintan Riau. Karya Ilmiah Institut Pertanian Bogor. 139 hlm.

Austin, B. & D.A. Austin. 2007. Bacterial Fish Pathogens: Diseases of Farm and Wild Fish. Praxis Publishing Ltd, Chichester, UK. 552 p.

Buckle, K.A. 2009. Ilmu Pangan. Universitas Indonesia Press. Jakarta. 365 hlm.

Cappucino J. G & N. Sherman. 2011. Microbiology a laboratory manual. Benjamin Cummings. San Fransisco. P 263-268.

Cob, C.Z., A. Arshad., S. J. Bujang., & A. M. Ghaffar. 2009. Partial And Temporal Variations In Strombus canarium (Gastropoda: Strombidae) Abundance At Merambong Seagrass Bed Malaysia. Sains Malaysiana. 43(4):503-511.

Dody S. 2007. Habitat dan sebaran spasial siput gonggong (Strombus turturella) di Teluk Klabat, Bangka Belitung. Prosiding Seminar Nasional Moluska 42 dalam Penelitian, Konservasi dan Ekonomi. Pusat Penelitian Oseanografi LIPI. Jakarta. 100 hlm.

Fardiaz, S. 1996. Mikrobiologi pengolahan pangan lanjut. Bogor: Departemen Pendidikan dan Kebudayaan, Direktorat Jenderal Pendidikan Tinggi, Pusat Antar Universitas Pangan dan Gizi, Intitute Pertanian Bogor.199 hlm.

Girard, F., I. Batisson., G. Frankel., J. Harel & J.M. Fairbrother. 2005. Interaction of enteropathogenic and Shiga-toxin producing Escherichia coli with porcine intestinal mucosa: role of intimin and Tir in adherence. Infection and Immunity. 73:6005-6016.

Hadioetomo, R.S. 1993. Mikrobiologi dasar dalam praktek. PT. Gramedia. Jakarta. Hlm.: 74-76.

Hanafiah, K. A. 2004. Rancangan percobaan, Edisi 3. Raja Grafindo Persada, Jakarta. Hlm 19.

Harborne, J.B. 2006. Metode Fitokimia Penuntun cara menganalisis Tumbuhan, Bandung: Penerbit ITB.354 hlm.

Hussain, T., A. Roohi., S. Munir., I. Ahmed., J. Khan., V. E. Hermann., K. Y. Kim., M. Anees. 2013. Biochemical characterization and identification of bacterial trains isolated from drinking water sources of Kohat Pakistan. African Journal of Microbiology Research. 7(16): 1579-1590.

Jawetz, E., J. L. Melnick & E.A.T. Adelberg. 2005. Mikrobiologi kedokteran. Edisi 22. Salemba Medika, Jakarta. Hal 205-209.

Lay, B. 1994. Analisis mikroba di laboratorium. Raja Grafindo Persada. Jakarta. Hlm 19-24.

Mayer, G. 2007. Medical Microbiology and Immunology. 3ed. Univ. of South Carolina School of Medicine, Carolina. P 205-215.

Tamsar., Emiyarti., & W. Nurgaya. 2013, Studi laju pertumbuhan dan tingkat eksploitasi kerang kalandue (Polymesoda erosa) pada daerah hutan mangrove di Teluk Kendari. Jurnal Mina Laut Indonesia. 02(6):11-16.

Sangi, M., M. R. J. Runtuwene., H. E. I. Simbala., & V. M. A. Makang. 2008. Analisis Fitokimia Tumbuhan Obat di Kabupaten Minahasa Utara. Chemistry Progress. 1:47-53.

Sudheesh, P. S., A. Al-Ghabshi., N. Al-Mazrooei., & S. Al-habsi. 2012. Comparative pathogenomics of bacteria causing infectious diseases in fish. International Journal of Evolutionary Biology. Article ID 457264:16 p.

Sutiknowati, L. I. 2013. Mikroba parameter kualitas perairan P. Pari untuk upaya pembesaran biota laut. Jurnal Ilmu dan Teknologi Kelautan Tropis. 5 (1):204-2018.

Syahrurachman, A. 1994. Buku ajar mikrobiologi kedokteran. Ed. Revisi. Fakultas Kedoketeran Universitas Indonesia, Jakarta. 429 hlm.

Yulianto R., Y. T. Adiputra., Wardiyanto & A. Setyawan. 2013. Perubahan jaringan organ ikan komet (Carrasius auratus) yang diinfeksi dengan Aeromonas hydrophila. E-Jurnal Rekayasa dan Teknologi Budidaya Perairan. 2(1): 197-203.

Zaidi, C. C., A. Arshad., M. I. Hanafi., J.S. Bujang & M. A, Ghaffar. 2008. Sexual polymorphism in a population of Strombus canarium Linnaeus, 1785 (mollusca: Gastropoda) at Merabong Shoal, Malaysia. Journal Zoological Studies. 47 (3): 7-11.



DOI: https://doi.org/10.22146/jfs.10346

Article Metrics

Abstract views : 707 | views : 1809

Refbacks

  • There are currently no refbacks.




Copyright (c) 2016 Jurnal Perikanan Universitas Gadjah Mada

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Perikanan Universitas Gadjah Mada Indexed by:

 

 

View My Stats