Efektivitas Metode Ekstraksi DNA pada Daun Segar dan Kering dari Tanaman Obat

Enny Rimita Sembiring; Rerenstradika Tizar Terryana; Yuliana Galih Dyan Anggraheni; Amalia Prihaningsih; Irmanida Batubara; Waras Nurcholis; Taopik Ridwan; Rikno Harmoko

doi:10.22146/veg.78957

Efektivitas Metode Ekstraksi DNA pada Daun Segar dan Kering dari Tanaman Obat

https://doi.org/10.22146/veg.78957

Enny Rimita Sembiring^(1*), Rerenstradika Tizar Terryana⁽²⁾, Yuliana Galih Dyan Anggraheni⁽³⁾, Amalia Prihaningsih⁽⁴⁾, Irmanida Batubara⁽⁵⁾, Waras Nurcholis⁽⁶⁾, Taopik Ridwan⁽⁷⁾, Rikno Harmoko⁽⁸⁾

(1) Badan Riset dan Inovasi Nasional
(2) Badan Riset dan Inovasi Nasional
(3) Badan Riset dan Inovasi Nasional
(4) Badan Riset dan Inovasi Nasional
(5) Institut Pertanian Bogor
(6) Institut Pertanian Bogor
(7) Institut Pertanian Bogor
(8) Badan Riset dan Inovasi Nasional
(*) Corresponding Author

Abstract

Tanaman obat merupakan salah satu tanaman yang sering menjadi target studi molekuler. Pada studi molekuler, ekstraksi dan purifikasi DNA genom merupakan tahap awal yang akan menentukan analisis selanjutnya. Oleh sebab itu, dibutuhkan metode ekstraksi DNA yang mampu menghasilkan DNA dengan kuantitas dan kualitas yang baik. Tujuan penelitian ini adalah untuk mengetahui efektivitas metode ekstraksi DNA dari daun segar maupun daun yang dikeringkan dari tanaman obat. Sebanyak sembilan genotip tanaman obat yang digunakan pada penelitian ini yaitu tiga genotip Justicia gendarussa, dua genotip Orthosiphon aristatus, dan masing-masing satu genotip Adenostemma lavenia, Adenostemma platyphyllum, Adenostemma madurense, dan Curcuma xanthorrhiza. Metode ekstraksi DNA genom yang digunakan adalah metode CTAB dengan beberapa modifikasi. Hasil penelitian DNA genom dari daun segar dan kering menunjukkan kuantitas, kualitas, dan dapat teramplifikasi dengan baik menggunakan primer RAPD dan penanda DNA.

Keywords

ekstraksi DNA; daun segar; daun kering; modifikasi metode CTAB

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DOI: https://doi.org/10.22146/veg.78957