Artikel penelitian
Vol 15 No 2 (2021): Volume 15, Number 2, 2021
The effect of nutrients mixture on The biomass and lipid production from microalgae Botryococcus braunii mutated by UV-C rays
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, 60111 Surabaya, Indonesia
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, 60111 Surabaya, Indonesia
Department of Chemical Engineering, Institut Teknologi Sepuluh Nopember, 60111 Surabaya, Indonesia
Abstrak
Nutrisi merupakan salah satu faktor terpenting dalam pertumbuhan mikroalga. Penelitian ini dilakukan untuk mempelajari pengaruh campuran nutrisi terhadap produksi biomassa dan lipid Botryococcus braunii. Mikroalga B. braunii dibudidayakan dalam media nutrisi komersial kombinasi pupuk pertanian amonium sulfat (ZA), urea, dan tripel superfosfat (TSP). Sebelum proses budidaya, B. braunii dipaparkan dengan sinar UV-C (254 nm) selama 3 menit. Konsentrasi dan jenis pupuk sebagai sumber nitrogen dibagi menjadi empat jenis campuran yaitu FM-1, FM-2, FM-3, dan FM-4 dibandingkan dengan unsur hara Walne untuk mempelajari pengaruhnya terhadap pertumbuhan mikroalga dan lipid. FM-1 yang terdiri dari 150 mg/L ZA, 7,5 mg/L urea, dan 25 mg/L TSP menghasilkan pertumbuhan terbaik untuk strain mikroalga asli dan mutasi dibandingkan dengan nutrisi Walne dan campuran nutrisi lainnya. Mikroalga yang bermutasi menunjukkan pertumbuhan yang lebih sedikit dibandingkan strain mikroalga asli. Namun proses mutasi secara signifikan meningkatkan kandungan lipid pada mikroalga. Pada strain mikroalga asli, FM-4 yang terdiri dari 136,3 mg/L urea dan 50 mg/L TSP menghasilkan lipid terendah yaitu 8,96%. Setelah dipaparkan sinar UV-C, lipid pada medium FM-4 meningkat menjadi 55,11%. Hasil penelitian menunjukkan bahwa penggunaan pupuk komersial dan paparan sinar UV-C pada mikroalga mempunyai potensi tinggi dalam penyiapan lipid sebagai bahan baku biodiesel yang dapat diterapkan secara efektif pada budidaya mikroalga skala besar.
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