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Biodiesel consists of various fatty acid esters which come from vegetable oil. More than 30 types of plants in Indonesia are potential to produce vegetable oils. One of the vegetable oils came from rubber seed. Therefore, utilization of rubber seed (Hevea Brasiliensis), as raw material for biodiesel was the precise breakthrough to add value to rubber plantation. This research aimed to determine the pattern of collection of oil of rubber seed maximally and to obtain the condition of production process of biodiesel fulfilling standard of SNI and ASTM. Biodiesel production was done in the prototype with a capacity of 20 liter/hour. The esterification process was conducted at 105°C using 10% methanol and acid catalyst for 90 minutes. Trans-esterification process was performed in an oscillating flow reactor with a catalyst dose of 1% oil weight and methanol as much as 15% oil weight. The effect of temperature and reaction time on product yield and quality were investigated. Purification of biodiesel was done in a vacuum system. Results from the present study showed that the yield of kernel through the process was up to 53% of the rubber seed weight. Meanwhile, the amount of oil could be extracted from the kernel was up to 56% of the kernel weight. The characteristic of biodiesel resulted from the process was in accord with that of the standard oil; density of 0.8565 g/ml, acid value 0.49, iodine value 62.88, ester fraction 97.2%, flash point 178C, heat of combustion 16,183 J/g.