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The presence of H2S in natural gas is very detrimental to ammonia industry because it can poison and deactivate steam reforming catalysts. In the ammonia plant Pusri-IB PT. Pusri Palembang, H2S was separated in the Desulfurizer Unit (201-D) by adsorption using ZnO adsorbent at low temperature (28 ° C). Unfortunately, in this process the ZnO adsorbent cannot be regenerated so that within one year the ZnO adsorbent will be saturated with sulfur. The alternative process of H2S separation is to dissociate H2S into its constituent elements (hydrogen and sulfur) with catalytic process. The magnesium oxide catalyst was chosen because magnesium oxide is a metal oxide compound widely known in the catalysis process and has two active sites. The highest H2S conversion that can be achieved by MgO catalyst is 92.29%. Unlike ZnO, MgO does not absorb H2S, but catalyzes the dissociation of H2S into hydrogen and solid sulfur without being changed consumed by the reaction itself so that the MgO catalyst has a longer life time than the ZnO adsorbent.