Topical gel preparation from Staphylococcus epidermidis isolate extract accelerated healing on incision wound model in rats

Chaerul Fadly Mochtar; Muh Irham Bakhtiar; Hasyrul Hamzah; Aldo Pratama; Jamiatur Rasyidah; Juniar Rakhmatia Indriyani; Muhammad Rizky Alfiannur; Nadhifah Suci Amalia; Nur Octaviany Putri; Nur Lutfiah Damis

doi:10.22146/ijpther.11510

Chaerul Fadly Mochtar Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan/ Indonesian Biofilm Research Collaboration Center (IBRCC), Sleman, Yogyakarta/ Faculty of Sports and Health Sciences, Universitas Negeri Makassar, Makassar, South Sulawesi, Indonesia
Muh Irham Bakhtiar Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan/ Indonesian Biofilm Research Collaboration Center (IBRCC), Sleman, Yogyakarta
Hasyrul Hamzah Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan/ Indonesian Biofilm Research Collaboration Center (IBRCC), Sleman, Yogyakarta https://orcid.org/0000-0001-5806-1760
Aldo Pratama Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan
Jamiatur Rasyidah Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan
Juniar Rakhmatia Indriyani Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan
Muhammad Rizky Alfiannur Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan
Nadhifah Suci Amalia Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan
Nur Octaviany Putri Faculty of Pharmacy, Universitas Muhammadiyah Kalimantan Timur, Samarinda, East Kalimantan
Nur Lutfiah Damis Faculty of Pharmacy, Universitas Muslim Indonesia, Makassar, South Sulawesi

DOI: https://doi.org/10.22146/ijpther.11510

Keywords: gel preparation, Staphylococcus epidermidis, wound healing, physical stability, topical

Abstract

A wound is a disorder of skin integrity caused by physical trauma or disease. Wound healing is a natural restorative response to tissue injury. It is a complex dynamic physiological process involving several overlapping phases. Although wound management has been applied, however, some time it is associated with
major drawbak including invasiveness, antibiotic resistance, and high costs. Natural medicines are being developed for wound management to resolve the drawback. Commensal Staphylococcus epidermidis was proven to play a role in natural wound healing process. This study aimed to investigate the wound healing
activity of a topical gel preparation containing S. epidermidis isolate extract on an incision wound model in rats. The methanol extract of S. epidermidis isolate was prepared by maceration and used as an active ingredient of the topical gel using NaCMC as a gelling agent. A physical stability test was carried out by assessing
organoleptic properties, pH value, homogeneity, dispersion, and viscosity of the gel preparation. The gel preparation was then topically applied once-daily for 11 d on incision wound model of male white rats (Rattus norvegicus). The wound healing was observed daily and calculated using Morton’s formula. No
significant change in the physical stability parameters of the gel preparation was observed during observation on day 1, 7, and 14 (p>0.05). A significant increase in wound healing after the application of the gel preparation compared to negative control was observed (p<0.05). In contrast, no significant difference in
wound healing between the gel preparation and the positive control group was observed (p>0.05). In conclusion, the topical gel preparation from S. epidermidis isolate extract accelerates healing on incision wound models in rats.

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