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Combination effectiveness of glucomannan and moringa oleifera leaf extract on lipid profil of hypercholesterolemia rats
https://doi.org/10.22146/teknosains.82693 Dini Prastyo Wati(1*), Endang Setyaningsih(2)
(1) Biology Education Departement, Faculty of Teacher Training and Education, Muhammadiyah University of Surakarta
(2) Biology Education Departement, Faculty of Teacher Training and Education, Muhammadiyah University of Surakarta
(*) Corresponding Author
Abstract
cholesterol levels in the blood. This study aims to determine the effectiveness of the combination of glucomannan (Amorphophallus muelleri Blume) and Moringa oleifera leaf extract on the lipid profile of hypercholesterolemic Wistar rats. This research design used True experimental with a pretest-posttest control group design. The animal used in this study was 24 male Wistar rats (Rattus norvegicus) aged 8 weeks divided into 2 control groups and 6 treatment groups of glucomannan and Moringa oleifera leaf extract. The results of this study showed that there were significant changes in TC, TG, LDL-C, and HDL-C levels (p<0.05). It can be concluded that the combination of glucomannan (Amorphophallus muelleri Blume) and Moringa oleifera leaf extract can change the lipid profile effectively in the P5 (80mg/kgBW GAmB: 120mg/kgBW MoEL) and P4 (120mg/kgBW GAmB:80mg/kgBW MoEL ) as the most influential group in reducing TG, TC, LDL-C levels and increasing HDL-C levels.
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[1] Soran, H., Schofield, J., & Adam, S. 2018. Guidelines Hypercholesterolaemia – practical information for non-specialists. Archieve of Medical Science, vol. 14, no. 1, pp. 1–21.
[2] A. S. Harti, Biokimia Kesehatan. Yogyakarta: Nuha Medika, 2018.
[3] J. Ardian, M. T. Jauhari, and B. F. Rahmiati, “Pengaruh Pemberian Jus Jambu Biji Merah terhadap Penurunan Kadar Ldl (Low Density Lipoprotein) dan Kolesterol Total,” Nutrition of Journal Pangan, Gizi, Kesehatan, vol. 1, no. 1, pp. 26–34, 2020, doi: 10.30812/nutriology.v1i1.733.
[4] I. G. A. A. P. Swastini, “Gambaran kolesterol total pada lansia di Puskesmas I Denpasar Selatan,” Meditory: The Journal of Medical Laboratory., vol. 9, no. 2, pp. 68–77, 2021, doi: 10.33992/m.v9i2.1526.
[5] Y. Sunardi, Disiplidemia : Nutrisi untuk Mengatasi Gangguan Kolesterol dan Komplikasinya. Yogyakarta: Rapha Publishing, 2019.
[6] Riskesdas, Laporan Nasional Badan Penelitian Kesehatan Kementrian RI tahun 2018, vol. 44, no. 8. 2018. [Online]. Available: http://www.yankes.kemkes.go.id/assets/downloads/PMK No. 57 Tahun 2013 tentang PTRM.pdf
[7] E. Lestari and Lagiono, “Pemanfaatan Tanaman Sebagai Obat Oleh Masyarakat Desa Karang Dukuh Kecamatan Belawang Kabupaten Barito Kuala,” Jurnal Hayati, vol. 4, no. 3, pp. 114–119, 2018.
[8] V. N. A. Kasim and Z. K. Yusuf, Tumbuhan Obat Berbasis Penyakit. Gorontalo: CV Athra Samudra, 2020.
[9] E. S. Astuti, S. Suryati, S. Bahri, M. Masrullita, and M. Meriatna, “Pengaruh Waktu dan Suhu Perebusan pada Umbi Porang (Amorphophallus Muelleri Blume) Menggunakan Larutan NaHCO3 terhadap Penurunan Kadar Kalsium Oksalat,” J. Teknol. Kim. Unimal, vol. 11, no. 1, p. 1, 2022, doi: 10.29103/jtku.v11i1.7243.
[10] K. Al-Ajlouni, F. Paul D, and P. Alexandra, “Glucomannan for food packaging biofilms- faceting of the polymer film,” in iarigai Athens Greece 2021 47th annual conference Printing in the Digital Era CONFERENCE PROCEEDINGS, 2021, pp. 123–136.
[11] M. Alamsyah, “Pengaruh glukomanan terhadap penurunan risiko penyakit stroke iskemik,” Jurnal Ilmu Kesehatan Sandi Husada, vol. 10, no. 2, pp. 292–298, 2019.
[12] T. I. Urli, T. Hariyanto, and N. Dewi, “Pengaruh pemberian tepung porang (Amorphophallus muelleri Blume) terhadap kadar HDL pada tikus (Rattus novergicus) Strain Wistar DM Tipe 2,” Journal of Nursing News., vol. 2, no. 2, pp. 31–37, 2017.
[13] B. Nugraheni, I. M. Cahyani, and K. Herlyanti, “Efek Pemberian Glukomanan Umbi Porang (Amorphophallus oncophyllus Prain Ex Hook. F.) terhadap Kadar Kolesterol Total Darah Tikus yang diberi Diet Tinggi Lemak,” Jurnal Ilmu Farmasi dan Farmasi Klinis, vol. 11, no. 2, pp. 32–36, 2014.
[14] F. G. Winarno, Tanaman Kelor (Moringa oleifera ) Nilai Gizi, Manfaat dan Potensi Usaha. Jakarta: PT Gramedia Pustaka Utama, 2018.
[15] L. Bao, L. Hu, Y. Zhang, and Y. Wang, “Hypolipidemic effects of flavonoids extracted from Lomatogonium rotatum,” Evidence-based Complementary & Alternative Medicine, vol. 11, no. 4, pp. 1417–1424, 2016, doi: 10.3892/etm.2016.3038.
[16] S. Salvamani, B. Gunasekaran, M. Y. Shukor, N. A. Shaharuddin, M. K. Sabullah, and S. A. Ahmad, “Anti-HMG-CoA reductase, antioxidant, and anti-inflammatory activities of amaranthus viridis leaf extract as a potential treatment for hypercholesterolemia,” Evidence-based Complementary & Alternative Medicine, vol. 2016, 2016, doi: 10.1155/2016/8090841.
[17] C. A. Aprilia, “Efektivitas Hipolipidemia dan Antioksidan Ekstrak Daun Binahong pada Tikus Putih yang Diinduksi Pakan Hiperkolesterol,” Yars Medical Journal, vol. 25, no. 3, p. 150, 2018, doi: 10.33476/jky.v25i3.362.
[18] M. Vergara-Jimenez, M. M. Almatrafi, and M. L. Fernandez, “Bioactive components in Moringa oleifera leaves protect against chronic disease,” Antioxidants, vol. 6, no. 4, pp. 1–13, 2017, doi: 10.3390/antiox6040091.
[19] A. A. Khamis, A. F. Salama, M. E. Kenawy, and T. M. Mohamed, “Regulation of hepatic hydroxy methyl glutarate – CoA reductase for controlling hypercholesterolemia in rats,” Biomedicine and Pharmacotheraphy, vol. 95, no. July, pp. 1242–1250, 2017, doi: 10.1016/j.biopha.2017.09.071.
[20] M. G. Rajanandh, M. N. Satishkumar, K. Elango, and B. Suresh, “Moringa oleifera Lam. A herbal medicine for hyperlipidemia: A pre-clinical report,” Asian Pacific Jornal of Tropical Disease. Vol, vol. 2, no. SUPPL2, pp. S790–S795, 2012, doi: 10.1016/S2222-1808(12)60266-7.
[21] V. C. G. G. J. Seenis, S. Bloembergen, and P. . Eyma, Flora, Terjemahan. Jakarta, 1992.
[22] C. A. Backer and V. D. B. R.C.B., Flora of Java (Spermatophytes only). Netherlands: N.V.P. Noordhoff-Groningen, 1963.
[23] G. Pasaribu, N. Hastuti, L. Efiyanti, T. K. Waluyo, and G. Pari, “Optimasi Teknik Pemurnian Glukomanan pada Tepung Porang (Amorphophallus muelleri Blume),” J. Penelit. Has. Hutan, vol. 37, no. 3, pp. 201–208, 2019.
[24] Y. Anas, A. Imron, and S. I. Ningtyas, “Ekstrak Daun Kelor ( Moringa Oleifera Lam.) Sebagai Peluruh Kalsium Batu Ginjal Secara In Vitro,” Jurnal Ilmu Farmasi dan Farmasi Klinis., vol. 13, no. 2, pp. 7–15, 2016.
[25] N. A. Nurulita, E. Sundhani, I. Amalia, F. Rahmawati, N. Nurhayati, and D. Utami, “Uji Aktivitas Antioksidan dan Anti-aging Body Butter dengan Bahan Aktif Ekstrak Daun Kelor ( Antioxidant and Anti-aging activity of Moringa Leaves Extract Body Butter ),” Jurnal Ilmu Kefarmasian Indonesia, vol. 17, no. 1, pp. 1–8, 2019.
[26] Susanty, S. A. Yudistirani, and M. Islam Bahrul, “Metode Ekstraksi Untuk Perolehan Kandungan Flavonoid Tertinggi dari Ekstrak Daun Kelor ( Moringa oleifera Lam.),” Konversi, vol. 8, no. 2, pp. 31–36, 2019.
[27] E. Suryanto and F. Wehantouw, “Aktivitas Penangkap Radikal Bebas dari Ekstrak Fenolik Daun Sukun (Artocarpus altilis F.),” Chemistry Progress, vol. 2, no. 1, pp. 1–7, 2009.
[28] AOAC, Official Methods of Analysis of Association of Official Analytical Chemist. Virginia USA, 1995.
[29] C. Nissa and I. J. Madjid, “Potensi glukomanan pada tepung porang sebagai agen anti-obesitas pada tikus dengan induksi diet tinggi lemak,” J. Gizi Klin. Indones., vol. 13, no. 1, p. 1, 2016, doi: 10.22146/ijcn.22751.
[30] S. Nahar, F. M. Faisal, and J. Iqbal, “Antiobesity activity of Moringa oleifera leaves against high fat diet-induced obesity in rats,” Int. J. Basic Clin. Pharmacol., vol. 5, no. 4, pp. 1263–1268, 2016, doi: 10.18203/2319-2003.ijbcp20162427.
[31] S. Aminah and M. Yusuf, “Efesiensi Pakan, Berat Badan dan Panjang Tulang Tikus yang Mengkonsumsi Kejale Selama 6 Minggu,” in The 2nd University Research Coloquium, 2015, pp. 451–459.
[32] D. Sari, R. Widyasari, and A. Taslima, “Penentuan Kadar Flavonoid Total Ekstrak Etanol Jamur Susu Harimau (Lignosus rhincerus),” Jurnal Farmasi Udayana., vol. 10, no. 1, pp. 23–30, 2021.
[33] W. Werdiningsih, N. T. Pratiwi, and N. Yuliati, “Determination of 70% Ethanol Extract Flavonoid Total Lavels Binahong (Anredera Cordifolia [Ten] Steenis) Leaves In Palem Village, Tanjunganom, Kab. Nganjuk,” Sintetis, vol. 3, no. 2, pp. 54–61, 2022.
[34] E. Apriyati, A. Murdiati, and P. Triwitono, “Effect of Meceration Time on Flavonoid Content and Antioxidant Activity of Moringa Leaf Extract,” Jurnal Teknologi Pangan., vol. 16, no. 2, pp. 116–123, 2022.
[35] B. Nugraheni and E. Sulistyowati, “Analisi kimia, makronutrien dan kadar glukomanan pada tepung umbi porang (Amorphalus konjac K. Koch.) setelah dihilangkan kalsium okslatnya menggunakan NaCl 10%,” in Repository STIFAR, 2018, pp. 92–101.
[36] N. Saleh, S. A. Rahayuningsih, B. S. Radjit, E. Ginting, D. Harnowo, and I. M. J. Mejaya, Tanaman Porang. 2015. [Online]. Available: http://balitkabi.litbang.pertanian.go.id/publikasi/petunjuk-teknis/booklet/2217-tanamanporang.html
[37] Sumarwoto, “Review : Constituent of Mannan of Iles-iles (Amorphophallus muelleri Blume.),” Bioteknologi, vol. 4, no. 1, pp. 28–32, 2007.
[38] J. K. da Silva, C. B. B. Cazarin, Â. G. Batista, and M. Maróstica, “Effects of passion fruit (Passiflora edulis) byproduct intake in antioxidant status of Wistar rats tissues,” Lwt-Food Science and Technology, vol. 59, no. 2P2, pp. 1213–1219, 2014, doi: 10.1016/j.lwt.2014.06.060.
[39] H. Rusmini, D. F. Putri, H. Hidayat, and D. Risandy, “Pengaruh Madu Ceiba Pentandra Terhadap Kadar LDL Tikus Rattus Norvegicus Yang Diberi Diet Tinggi Lemak,” Jurnal Ilmu Kesehatan Sandi Husada, vol. 11, no. 1, pp. 479–489, 2020, doi: 10.35816/jiskh.v11i1.331.
[40] J. Bresnahan, Biological and Physiological Data on Laboratory Animal. USA: Kansas State University, 2004.
[41] N. Susanti, “Suplementasi Tepung Porang (Amorphophallus Muelleri Blume) Sebagai Nutraceutical Dalam Manajemen Diabetes Mellitus Tipe 2,” El-Hayah, vol. 5, no. 1, pp. 9–16, 2014.
[42] A. Fairudz and K. Nisa, “Pengaruh Serat Pangan terhadap Kadar Kolesterol Penderita Overweight,” Majority, vol. 4, no. 8, pp. 121–126, 2015.
[43] O. Okwari, K. Daofunjo, A. Asuk, E. Alagwu, and M. C, “Anti-Hypercholesterolemic and Hepatoprotective effect of Aqueous Leaf Extract of Moringa oleifera in Rats Fed With Thermoxidized Palm Oil Diet,” IOSR Journal of Pharmacy and Biological Science, vol. 8, no. 2, pp. 57–62, 2013.
DOI: https://doi.org/10.22146/teknosains.82693
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