Herniyati Herniyati(1*)

(1) Fakultas Kedokteran Gigi Universitas Jember
(*) Corresponding Author


The objective of  research is  to determine the expression of osteocalcin on osteoblasts during orthodontic tooth movement after administration robusta coffee extract. Materials and Methods: 16 rats  were divided into 2 groups: group K: rats applied with orthodontic mechanical force (OMF) and  group P: OMF +  coffee robusta extract of 20mg /100 g of BW. OMF was conducted by applying  ligature wire on permanent maxillary right first molar and both permanent maxillary incisivus. Subsequently, the permanent maxillary right first molar  moved to mesial with Ni-Ti orthodontic closed coil spring. Observations were made on day 15 by immunohistochemical examination to determine the expression of osteocalcin. Results: Robusta coffee extract  improved the expression of osteocalcin in the compression and tension areas (p <0.05). Expression of osteocalcin in  tension area  larger than that in compression area (p <0.05). Conclusion: The administration of Robusta coffee extract  increases the expression of osteocalcin, which can increase alveolar bone remodeling.


Alveolar bone remodeling; Orthodontic tooth movement; Osteocalcin; Robusta coffee

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Baek,  K.H., Oh, K.W., Lee, W.Y., Lee ,S.S., Kim, M.K.  and Kwon, H.S. 2010. Association of oxidative  stress with postmenopausal osteoporosis and effects of hydrogen peroxide on osteoclast formation in human bone marrow cell cultures. Calcif Tissue Internat.  87 (3): 226-35.

 Banfi, G., Iorio, E. L. and  Corsi, M.M. 2008. Oxidative stress, free radicals and bone remodeling. Clinical Chemistry and Laboratory Medicine: CCLM/FESCC 46(11): 1550-1555.

Bin, H.S., Jeong, J. H. and  Choi, U. K. 2013. Chlorogenic acid promotes osteoblastogenesis in human adipose tissue-derived mesenchymal stem cells. Food Science, and Biotechnology 22: 107-112.

 Brooks, P.J., Nilforoushan, D., Manolson, M. F., Simmons, C, A. and  Gong, S.G.  2009. Molecular markers of early orthodontic tooth movement.  Angle Orthodontis  79( ): 1108–1113.

 D’Apuzzo , F., Cappablanca , S., Clavarella, D., Monsurro, A., Biavati, A.S. and Perillo, L.  2013.. Biomarkers of Periodontal Tissue Remodelling during Orthodontic Tooth Movement in Mice and Men, Overview and Clinical Relevance, Scien.World J.

 Farah, A. and Donangelo, C.M.  2006. Phenolic compounds in coffee. Braz. J. Plant Physiol. 18:  23–36.

 Jem, S. S., Lung, C. K., Hui, S. S., Tsung,  Y.Y., Wen, S.H. and Ming, C. K. 2013. Caffeine regulates osteogenic differentiation and mineralization of primary adipose-derived stem cells and bone marrow stromal cell line.  International Journal of Food Sciences and nutritionl 64(4): 429-436.

 Jianru,  Y., Zhang,  L.,, Yan, B., Yang, L., Li, Y. and Zhao, Z. 2012. Drinking coffee may help accelerate orthodontic tooth movement.  Department of Dental Implants, State Key Laboratory of Oral Diseases, West China Stomatology Hospital, Sichuan University, Chengdu, Sichuan, China, 3(2): 72-75.

 Garlet , T.P., Coelho, U., Silva, J.S. and Garlet, G.P. 2007. Cytokine expression pattern in compression and tension sides of the periodontal ligament during orthodontic tooth movement in humansEurop J of oral Sciences 115( 5): 355–362.

 Hadjidakis, D.J. and  Androulakis, I.I.  2006.  Bone remodeling.  Annals of the New York Academy of Sciences 1092: 385–396.

 Hannon, R,A. and Eastell, R. 2006. Bone markers and current laboratory assays. Cancer Treatment Review 32(1): 7–14.

 Kawakami , M. and  Yamamoto,  T.T.  2004. Local injection of 1,25-dihydroxyvitamin D3 enhanced bone  formation for tooth stabilization after experimental tooth movement in rats. J Bone Miner Metab  22: 541–546.

  Krisnan, V. and  Davidovitch,  Z.  2009. The role of tumor necrosis factor type 1 in orthodontic tooth movement.  J Dent Res 88(7):  597–608.

 Meikle, M.C. 2006. The tissue, cellular, and molecular regulation of orthodontic tooth movement: 100 years after Carl Sandstedt.  Eur J Ortho ;28(3): 221–240.

 Narmada, I.B.  2010.  Efek Biostimulasi  Laser Intensitas Rendah Terhadap Kecepatan Pergerakan Gigi Otodonti. Disertasi. Prodram Pasca Sarjana Universitas Airlangga. Surabaya.

Reis, A.S., Riberio,  L.G.R., Ocarino, N. M., Goes, A.M. and Serakides, R. 2015. Osteogenic potential of osteoblasts from neonatal rats born to mothers treated with caffeine throughout pregnancy. BMD Musculoskeletal Disorders 16: 10.

 Riancho, J.A. and Delgado-Calle, J. 2011. Osteoblast-osteoclast interaction mechanisms. Reumatologı´a Clı´nica   2:  S1–S4.

 Sprogar, S., Vaupotic, T., Cor,  A. and  Drevensek G.  2008. The endothelin system mediates bone modeling in the late stage of orthodontic tooth movement in rats.  Bone  43(4): .740-747.

 Sukendro ,S. 2013. Keajaiban Dalam Secangkir Kopi. Penerbit Media Pressindo. Yogyakarta.

 Tanaka, Y., Nakayamada, S. and  Okada, Y.  2005. Osteoblasts and osteoclasts in bone remodeling and inflammation. Current Drug Targets Inflammation and  Allergy  4: 325–328.

 Yashin,  A., Yashin, Y.,  Wang , J. Y. and Nemzer, B.  2013. Antioxidant and Antiradical Activity of Coffee. Antioxidants  2:  230-245.


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