* Corresponding Author
Viability of 7F2 pre-osteoblast after Sulawesi stingless bee (Tetragonula biroi) 1% propolis nanoemulsion extraction
https://doi.org/10.22146/majkedgiind.98860 Ratri Maya Sitalaksmi(1*), Nadya Rafika Amalia(2), Alexander Patera Nugraha(3), Theresia Indah Budhy(4), Nastiti Faradilla Ramadhani(5), Putri Cahaya Situmorang(6), Tengku Natasha Eleena binti Tengku Ahmad Noor(7), Khairul Anuar Shariff(8)
(1) Department of Prosthodontic, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
(2) Dental Health Science, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
(3) Department of Orthodontic Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
(4) Oral and Maxillofacial Pathology Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
(5) Dentomaxillofacial Radiology Department, Faculty of Dental Medicine, Universitas Airlangga, Surabaya, East Java, Indonesia
(6) Study Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Sumatera Utara, Medan, Indonesia
(7) Malaysian Armed Forces Dental Officer, 609 Armed Forces Dental Clinic, Kuching, Serawak, Malaysia
(8) School of Materials and Mineral Resources Engineering, Engineering Campus, Universiti Sains Malaysia Malaysia
(*) Corresponding Author
Abstract
Produced by a stingless bee (Tetragonula biroi) from Sulawesi Island, propolis is an active compound that requires further studies to determine its herbal-based medicinal applications. In addition, propolis is well known as a potential natural ingredient for herbal medicine with minimal side effects. The stingless honeybee, native to Sulawesi, produces propolis, a resin. Propolis from Trigona stingless bees in Sulawesi has the highest flavonoid content compared to Apis mellifera bees. Flavonoids are the most common compounds contained in propolis. Propolis from stingless bees may have the potential to stimulate osteoblast cell proliferation and be responsible for bone regeneration. The objective of this study is to investigate the viability of the 7F2 pre-osteoblast cell line after administration of the stingless bee’s 1% propolis nanoemulsion extract (PNE). A 1% PNE was formulated by maceration methods and diluted into several concentrations of 100%, 50%, 25%, 12.5%, 6.25%, 3.125%, 1.56%, and 0.78%. A particle size analysis was done to examine the particle size and zeta potential of the 1% PNE. A Tetrazolium Technique assay was carried out to examine 1% PNE application on a 7F2 pre-osteoblast cell line on days 1, 3, and 7. The highest viability of 7F2 pre-osteoblast cells was at 0.78% concentration on day 7, and the lowest viability was observed at 100% concentration on day 1, with significant differences among groups (p < 0.05). A 1% PNE with a particle size of 151.28 to 182.2 nm and a zeta potential of −32.76 mV was successfully formulated. The 1% PNE was non-toxic to 7F2 pre-osteoblast cells, with a cell toxicity limit at a concentration of 1.56%.
Keywords
Full Text:
3. Ratri Maya SitalaksmiReferences
1. Salikha K, Narmada IB, Alida , Nugraha AP, Sari AF, Riawan W, et al. Anti-inflammatory effect of caffeic acid phenethyl ester supplementation on TNF-α and NF-κB expressions throughout experimental tooth movement in vivo. Journal of Pharmacy and Pharmacognosy Research. 2022; 10(6): 1037-1045.
2. Nugraha AP, Koendhori EB, Fahmi A, Wahyunitiari MR. Ethanolic extract of propolis from Indonesian stingless bee (Tetragonula biroi) is ineffective against ESBL-producing Klebsiella pneumoniae: An in vitro study and a brief review of propolis antibacterial and anti-inflammatory activity. World Journal of Advanced Research and Reviews. 2023; 20(03): 051–059.
doi: 10.30574/wjarr.2023.20.3.2381
3. Narmada IB, Putri PD, Lucynda L, Triwardhani A, Ardani IGAW, Nugraha AP. Effect of caffeic
acid phenethyl ester provision on fibroblast growth factor-2, matrix metalloproteinase-9
expression, osteoclast and osteoblast numbers during experimental tooth movement
in wistar rats (Rattus norvegicus). Eur J Dent. 2021; 15(2): 295-301. doi: 10.1055/s-0040-1718640
4. Ramadhan R, Syafrizal, Kusuma IW, Paramita S, Amen Y, Kim YU, et al. Cytotoxicity
evaluation of phytochemicals from stingless bee (Tetragonula biroi) propolis. Toxicon.
2024; 237: 107352. doi: 10.1016/j.toxicon.2023.107352
5. Zullkiflee N, Taha H, Usman A. Propolis: its role and efficacy in human health and
diseases. Molecules. 2022; 27(18): 6120. doi: 10.3390/molecules27186120
6. Ariestania V, Hendrijantini N, Prahasanti C, Prasetyo E, Kuntjoro M, Sari RP, et al. Cytotoxicity of HA-TCP scaffold on human umbilical cord mesenchymal stem cells using MTT Assay. International Journal of Integrated Engineering. 2022; 14(2): 80-85.
7. Kuntjoro M, Prasetyo EP, Cahyani F, Kamadjaja MJK, Hendrijantini N, Laksono H, et al. Lipopolysaccharide’s cytotoxicity on human umbilical cord mesenchymal stem cells. Pesqui Bras Odontopediatria Clin Integr. 2020; 20: 1-7. doi: 10.1590/pboci.2020.153
8. Soesilawati P, Rizqiawan A, Roestamadji RI, Arrosyad AR, Firdauzy MAB, Kasim NHA. In vitro
cell proliferation assay of demineralized dentin material membrane in osteoblastic mc3t3-e1
cells. Clin Cosmet Investig Dent. 2021; 13: 443-449. doi: 10.2147/CCIDE.S313184
9. Hermawan JA, Uzmayana RAD, Rizqiawan A, Widyowati R, Tran TT. Viability of α-mangostin and Lipopolysaccharide (LPS) in 7F2 cells. Tropical Journal of Natural Product Research. 2023; 7(1): 2234-2236. doi: 10.26538/tjnpr/v7i1.22
10. Azhari AF, Kamadjaja DB, Rizqiawan A, Soesilowati P, Widyowati R, Tobiume K. The
potential of α-mangostin on TNF-α and OSX expression post inflammation induction
on osteoblast: an experimental in vitro study. Journal of International Dental and
Medical Research. 2022; 15(4): 1574-1579.
11. Nugraha AP, Triwardhani A, Sitalaksmi RM, Ramadhani NF, Luthfi M, Ulfa NM, et al. Phytochemical, antioxidant, and antibacterial activity of Moringa oleifera nanosuspension against peri-implantitis bacteria: an in vitro study. J Oral Biol Craniofac Res. 2023; 13(6): 720-726.
doi: 10.1016/j.jobcr.2023.09.004
12. Amin AA, Mahmoud KF, Salama MF, Longo V, Pozzo L, Seliem EI, Ibrahim MA. Characterization and stability evaluation of Egyptian propolis extract nano-capsules and
their application. Sci Rep. 2023; 13(1): 16065. doi: 10.1038/s41598-023-42025-0
13. Fitria A, Hanifah S, Chabib L, Uno AM, Munawwarah H, Atsil N, Pohara HA, Weuanggi
DA, Syukri Y. Design and characterization of propolis extract loaded self-nano emulsifying
drug delivery system as immunostimulant. Saudi Pharm J. 2021; 29(6): 625-634.
doi: 10.1016/j.jsps.2021.04.024
14. Ekeuku SO, Chin KY. Application of propolis in protecting skeletal and periodontal health-a
systematic review. Molecules. 2021; 26(11): 3156. doi: 10.3390/molecules26113156
15. Krismariono A, Wiyono N, Prahasanti C. Viability test of fish scales collagen from
oshphronemus gouramy on osteoblast cell culture. Journal of International Dental and
Medical Research. 2020; 13(2): 412-416.
16. Sularsih S, Soetjipto S, Rahayu RP. Cytotoxicity of combination chitosan with different molecular weight and ethanol extracted aloe vera using MTT Assay. IOP Conf Ser: Earth Environ Sci. 2019; 217(1): 012030. doi: 10.1088/1755-1315/217/1/012030
17. Soetojo A, Purnama D, Lunardhi CGJ, Widjiastuti I. Cytotoxicity test of 4-methacryloxyethyl
trimellitic anhydride-based dentine bonding material using acetone solution in dental pulp
fibroblast. Journal of International Oral Health. 2019; 11(4): 191-196.
doi: 10.4103/jioh.jioh_32_19
18. Jayakusuma A, Rizqiawan A, Pradana S, Soesilawati P, Tran T, Kamadjaja D. Alpha-mangostin post-induction of lipopolysaccharides on interleukin-6 and bone sialoprotein expression in osteoblast cells: An experimental in vitro study. Journal of International Oral Health. 2023; 15(3): 271-277. doi: 10.4103/jioh.jioh_194_22
19. Tantra I, Rizqiawan A, Sumarta N, Kamadjaja D, Soesilowati P, Rahman M, et al. Effect of α-mangostin on interleukin-11 and runtrelated transcription factor-2 gene expression on cell line osteoblast cultures induced with lipopolysaccharide. Contemp Clin Dent. 2023; 14(1): 68-71. doi: 10.4103/ccd.ccd_723_21
20. Dewa WJ, Handharyani E, Purwaningsih S, Mariya S. Expression of apoptosis marker
genes bax and bcl-2 in WiDr colon cancer cells treated with red eye sea snail (Cerithidea
obtusa) extract. Adv Anim Vet Sci. 2023; 12(2): 305-309.
doi: 10.17582/journal.aavs/2024/12.2.305.309
21. Kresnoadi U, Rahayu RP, Ariani MD, Soesanto S. The potential of natural propolis extract
combined with bovine bone graft in increasing heat shock protein 70 and osteocalcin on
socket preservation. Eur J Dent. 2020; 14(1): 31-37. doi: 10.1055/s-0040-1701921
22. Varton D, Widiyanti P, Wahyuningsih SPA, Aminatun. Biocompatibility testing of
hydroxyapatite-chitosan-chondroitin sulfate composite scaffold as bone graft. Journal of
International Dental and Medical Research. 2021; 14(1): 404-411.
23. Lesmaya YD, Kamadjaja DB, Wardana WM, Purwati. Cytotoxicity study of freezedried
bovine bone xenograft in human bone marrow mesenchymal stem cell. Asian Journal of Microbiology, Biotechnology and Environmental Sciences. 2018; 20: S62-S65.
DOI: https://doi.org/10.22146/majkedgiind.98860
Article Metrics
Abstract views : 2002
| 
views : 612
Refbacks
- There are currently no refbacks.
Copyright (c) 2024 Majalah Kedokteran Gigi Indonesia
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.