Identifikasi Kromatografi Lapis Tipis Sudamala (Artemisia vulgaris L.)

Ira Arundina; Theresia Indah Budhy S.; Muhammad Luthfi; Retno Indrawati

doi:10.22146/majkedgiind.9226

Identifikasi Kromatografi Lapis Tipis Sudamala (Artemisia vulgaris L.)

https://doi.org/10.22146/majkedgiind.9226

Ira Arundina^(1*), Theresia Indah Budhy S.⁽²⁾, Muhammad Luthfi⁽³⁾, Retno Indrawati⁽⁴⁾

(1) Departemen Biologi Oral, Fakultas Kedokteran Gigi Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
(2) Departemen Patologi Mulut dan Maksilofasial, Fakultas Kedokteran Gigi, Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
(3) Departemen Biologi Oral, Fakultas Kedokteran Gigi Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
(4) Departemen Biologi Oral, Fakultas Kedokteran Gigi Universitas Airlangga, Surabaya, Jawa Timur, Indonesia
(*) Corresponding Author

Abstract

Identification of Sudamala (Artemisia vulgaris L.) Thin Layer Chromatography. Oral squamous cell carcinoma is the type of cancer which is most frequently found in oral cavity. The primary risk factor of malignancy in oral cavity includes the habit of consuming tobacco and or alcohol. The plant sudamala (Artemisia vulgaris L.) is often used in the community as anti-tumor in digestive organ, including in oral cavity. However, there have been no studies on active ingredients playing the role as anti-cancer in oral cavity. The species are mostly from the genus Artemisia, while those generally growing in Indonesia are the species Artemisia vulgaris L. The objective of this study is to explain the identification by TLC of sudamala. The study was sudamala extraction, identification of sudamala extract, sudamala fractionation with Vacuum Column Chromatography and identification of sudamala fractionation with TLC (Thin Layer Chromatography). The result shows that the fractionation using hexane extract resulted in 11 fractions of n-hexane: ethyl acetate. The conclusion of this study is that the fraction of n-hexane: ethyl acetate (3:7, v/v) sudamala (Artemisia vulgaris L.) identified contains terpenoids compounds.

Keywords

Sudamala (Artemisia vulgaris L).; Identification; TLC; Oral Cancer

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References

Greenberg MS, Glick M. Burket’s oral medicine diagnosis and treatment. Hamilton: BC Decker Inc. 2003; 194-201.

Pindborg JJ. Kanker dan prakanker rongga mulut. Jakarta. EGC. 2000; 1-138.

Cotran RS, Kumar V, Collins T. Pathologic basic of disease. 7th ed. Philadelphia. WB Saunders Company. 2005; 260-325.

Padua LS, Bunyapraphatsara N, Lemmens RHMJ. Plant resources of south east asia. medicinal and poisonous plant jilid 1. Bogor Indonesia. 2000; 139-148.

Singh NP, Lai H. Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sciences.

; 70: 49-56.

Efferth T, Sauerbrey A, Olbrich A, Gebhart E. Molecular modes of action of artesunate in tumor cell lines. Molecular Pharmacology. 2003; 64(2): 382-394.

Dalimartha S. Atlas tumbuhan obat indonesia jilid 1. Trubus Agriwidya. Jakarta. 2005; 7-12.

Yan Z, Subbaramaiah K, Camilli T, Zhang F,Tanabe T, McCaffrey TA, Dannenberg AJ,Weksler BB. Benzo(a)pyrene induces the transcription of cyclooxygenase-2 in vascular smooth muscle cells. J Biological Chemistry.2000; 275(7): 4949-4955.

Elferaly, Farouk S, Elsohly, Hala N. Method for the isolation of artemisinin from artemisia annua. J Chrom. 1990; 355: 448-450.

Arundina I. Inhibitory effect of n-hexane: ethyl acetate fraction from artemisia vulgaris l. on cell culture of epithelial carcinoma of the mouth. Dental J. 2009; 42(1): 39-42.

Zhang S, Won YK, Ong CN, Shen HM. Anti cancer potential of sesquiterpene lactones bioactivity and molecular mechanisms. Curr Med Chem Anti Canc Agents. 2005; 5(3): 239-249.

Singh NP, Lai H. Selective toxicity of dihydroartemisinin and holotransferrin toward human breast cancer cells. Life Sciences.

; 70: 49-56.

Kim DH, Na HK, Oh TY, Kim WB, Surh YJ.Eupatilin a pharmacologically active flavone derived from artemisia plants induces cell cycle arrest in ras transformed human mammary epithelial cells. Biochemical Pharmacology.2004; 68: 1081-1087.

Jeong MA, Lee KW, Yoon DY, Lee HJ.Jaceosidin a pharmacologically active flavone derived from artemisia argyi inhibits phorbol ester induced upregulation of cox 2 and MMP 9 by blocking phosphorylation of ERK 1-2 in cultured human mammary epithelial cells.New York Academy of Sciences. 2007; 1095:458-466.

Kim MJ, Kim DH, Na HK, Oh TY, Shin CY,Surh YJ. Eupatilin a pharmacologically active flavone derived from artemisia plants induces apoptosis in human gastric cancer cells.Journal Environ Pathol Toxicol Oncology.2005; 24(4): 261-269.

Kim MJ, Kim DH, Lee KW, Yoon DY, Surh YJ. Jaceosidin induces apoptosis in ras transformed human breast epithelial cells through generation of reactive oxygen species.New York Academy of Sciences. 2007; 1095:483-495.

Martin KR, Trempus C, Saulnier M, Kari FW, Barrett JC, French JE. Dietary N acetyl l-cysteine modulates benzo(a)pyrene

induced skin tumors in cancer-prone p53 haploinsufficient Tg.Ac (v Ha-Ras) Mice. Carcinogenesis. 2001; 22(9): 1373-1378.

DOI: https://doi.org/10.22146/majkedgiind.9226