Effect of aloe vera on the proliferation phase of oral mucosal wound healing in rats

https://doi.org/10.22146/majkedgiind.77682

Ameta Primasari(1*), Yendriwati Yendriwati(2), Minasari Minasari(3), Erlin Kristiani Buaya(4), Sri Ayu Winda Madani(5), Kartika Nabila Sembiring(6), Rizka Hidayati(7)

(1) Department of Oral Biology, Faculty of Dentistry, University of Sumatera Utara, North Sumatra
(2) Department of Oral Biology, Faculty of Dentistry, University of Sumatera Utara, North Sumatra
(3) Department of Oral Biology, Faculty of Dentistry, University of Sumatera Utara, North Sumatra
(4) Department of Oral Biology, Faculty of Dentistry, University of Sumatera Utara, North Sumatra
(5) Department of Oral Biology, Faculty of Dentistry, University of Sumatera Utara, North Sumatra
(6) Department of Oral Biology, Faculty of Dentistry, University of Sumatera Utara, North Sumatra
(7) Department of Oral Biology, Faculty of Dentistry, University of Sumatera Utara, North Sumatra
(*) Corresponding Author

Abstract


Aloe vera is known as a medicinal plant containing an active substance acemannan thought to play a role in accelerating wound healing. This study aims to determine the effect of aloe vera extract on the proliferation phase of oral mucosa wound healing in rats by looking at epithelial thickening, epithelial gaps closure, growth of new blood vessels, and connective tissue growth. This research is a pure experimental study with a post-test only control group design. On the left buccal mucosa of the mouth of 24 white Wistar rats (n = 24), an incision was made by scalpel with 10 mm length and 2.5 mm depth. The rats were divided into 4 groups: groups 1 and 2 as controls, while groups 3 and 4 were applied with aloe vera extract made by maceration technique. Wound tissue was observed histologically with Hematoxylin-Eosin staining under a microscope with an image analyzer for measuring the thickness of epithelium. The analysis of epithelial gap width used morphometry, and the number of capillaries was counted manually. Connective tissue thickness was measured with Image-J software. These were carried out on days 7 and 14 after treatment and the data were analyzed with the independent t-test and Mann-Whitney test. The results showed that aloe vera extract had no significant effect on epithelial thickening on day 7 (p = 0.701) nor on day 14 (p = 0.639), but a significant effect was shown on epithelial gaps closing (p = 0.049), connective tissue thickening (p = 0.004), and capillary increase on day 7 (p = 0.049). It could be concluded that aloe vera extract could potentially accelerate the epithelial gaps closure, connective tissue thickening, and capillary increase during the proliferation phase of the oral mucosa wound healing.

Keywords


aloe vera extract; epithelialization; wound healing; mucosal tissue; angiogenesis

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References

1. Nanci A. Ten Cate’s Oral Histology: Development. J Oral Maxillofac Surg. 2018;
48: 98–99.

2. Primasari A, Sinulingga ER. Differences of wound healing in the buccal part and
oral mucosa labial after giving lemongrass extracts (Cymbopogon ciratus). Int J
Homeopath Nat Med. 2019; 5(1): 36-41. doi: 10.11648/j.ijhnm.20190501.16

3. Dawson DV, Drake DR, Hill JR, Brogden KA, Fischer CL, Wertz PW. Organization, barrier
function and antimicrobial lipids of the oral mucosa. Int J Cosmet Sci. 2013; 35(3): 220–
223. doi: 10.1111/ics.12038

4. Anura A. Traumatic oral mucosal lesions: a mini review and clinical update. Oral Health
Dent Manag. 2014; 13(2): 254-259.

5. Harper D, Young A, McNaught CE. The physiology of wound healing. United
Kingdom: Elsevier Ltd; 2014. 445–450.

6. Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound healing. J Chin Med Assoc.
2018; 81(2): 94–101. doi: 10.1016/j.jcma.2017.11.002

7. Brandão LM, Reis PRM, Araujo ACV, Santos MHAS, Miguel MP. Evaluation of
wound healing treated with latex derived from rubber trees and Aloe Vera extract in
rats 1. Acta Cirúrgica Bras. 2016; 31(9): 571. doi: 10.1590/S0102-865020160090000001

8. Kumar S, Yadav JP. Ethnobotanical and pharmacological properties of Aloe vera:
A review. J Med Plants Res. 2014; 8(48): 1387–1398. doi: 10.5897/JMPR2014.5336x

9. Sujatha G, Kumar GS, Muruganandan J, Prasad TS. Aloe vera in dentistry. J Clin
Diagnostic Res. 2014; 8(10): 1. doi: 10.7860/JCDR/2014/8382.4983

10. Budiyanto MAK. Efek ekstrak lidah buaya (Aloe vera) terhadap diameter penyembuhan
luka decubbitus pada tikus putih. Malang: Universitas Muhammadiyah Malang Press;
2017. 1.

11. Listari KM, Hasanah A. Comparison of Jatropha multifida L gel and Aloe Vera
gel to the number of blood vessels post gingivectomy. Malaysian Journal of Medicine
and Health Science. 2020; 16(12): 9346.

12. Ningsih JR, Haniastuti T, Handajani J. Reepitelisasi luka soket pasca pencabutan gigi
setelah pemberian gel getah pisang raja. Jurnal Ilmu Kedokteran Gigi. 2019; 168:
198–221.

13. Hajhashemi V, Ghannadi A, Heidari AH. Antiinflammatory and wound healing activities of
Aloe littoralis in rats. Res Pharm Sci. 2012; 7(2): 73-8.

14. Mustaqim A, Asri A, Almurdi. Pengaruh pemberian gel lidah buaya (Aloe vera)
terhadap gambaran histopatologi gaster tikus wistar yang diinduksi indometasin.
Jurnal Kesehatan Andalas. 2017; 6(3): 641-646. doi: 10.25077/jka.v6i3.751

15. Nazir F, Zahari A, Anas E. Pengaruh pemberian gel lidah buaya (Aloe vera)
terhadap jarak pinggir luka pada tikus Wistar. Jurnal Kesehatan Andalas. 2015;
4(3): 827–34. 10.25077/jka.v4i3.371

16. Hobson R. Vitamin E and wound healing: An evidence-based review. Int Wound J. 2016;
13: 331–5. doi: 10.1111/iwj.12295.

17. Zinder R, Cooley R, Vlad LG, Molnar JA. Vitamin A and wound healing. Nutr Clin
Pract. 2019; 34: 839–49. doi: 10.1002/ncp.10420.

18. Teplicki E, Ma Q, Castillo DE, Zarei M, Hustad AP, Chen J, Li J. The effects of aloe
vera on wound healing in cell proliferation, migration, and viability. Wounds. 2018;
30(9): 263–268.

19. Kalangi SJR. Khasiat Aloe vera dan madu topikal pada re-epitelisasi dan pembentukan
jaringan granulasi luka eksisi kulit telinga kelinci. J Biomedik. 2014; 5(3): 171–80.
doi: 10.35790/jbm.5.3.2013.4338

20. DiPietro LA. Angiogenesis and wound repair: when enough is enough. J Leukoc Biol. 2016;
100(5): 979–984. doi: 10.1189/jlb.4MR0316-102R.

21. Ruauw EF, Wantania FE, Leman MA. Pengaruh lidah buaya (Aloe Vera) terhadap waktu
penutupan luka sayat pada mukosa rongga mulut tikus Wistar. Pharmacon. 2016; 5(2): 22–28.



DOI: https://doi.org/10.22146/majkedgiind.77682

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