Effect of 4% and 15% moringa leaf extract gel on gingival wound healing in rats

https://doi.org/10.22146/majkedgiind.76416

Eka Pramudita Ramadhany(1*), I Gusti Agung Dyah Ambarawati(2), Muhammad Rafif Musyaffa(3)

(1) Department of Periodontics, School of Dentistry, Faculty of Medicine, Universitas Udayana, Bali
(2) Department of Oral Medicine, School of Dentistry, Faculty of Medicine, Universitas Udayana, Bali
(3) Dentistry Program and Professional Education, Faculty of Medicine, Universitas Udayana, Bali
(*) Corresponding Author

Abstract


Traumatic dental procedures such as incisions and gingival flaps are the leading causes of injury to the gingival structure. One of natural ingredients that can potentially accelerate the wound healing process is Moringa leaves Moringa oleifera). Moringa leaf has several active compounds, one of which is flavonoids, which can be an antiinflammatory and antibacterial agent, and increase collagen synthesis. A randomized posttest-only control-group design was used in this study. 48 wistar rats were categorized into four groups: CMC-Na gel, hyaluronic acid, 4% Moringa leaf extract gel, and 15% Moringa leaf extract gel. The samples were then euthanized on days 1, 3, 5, and 7. The two-way ANOVA test described significant differences (p < 0.05) for all the components of the observation (neutrophils, fibroblasts, angiogenesis, and epithelial thickness). The lowest mean number of neutrophils, the highest mean number of fibroblasts, and the highest mean of angiogenesis were found in the 15% Moringa extract group on the 7th day. The highest mean epithelial thickness was found in the use of 4% Moringa extract on the 5thday. The microscopic images showed that the treatment group gained more effective wound healing processes than the control group. The microscopic image showed that, in terms of neutrophils, fibroblasts, and angiogenesis, 15% Moringa extract was more effective for wound healing compared to 4% Moringa extract.

Keywords


gingival wound; Moringa oleifera; topical gel; wound healing

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References

1. Michael G. N, Takei H, R. Klokkevold P, Carranza F. Newman and carranza’s clinical
periodontology. Elsevier Health Sciences; 2018. 182-219.

2. Nussbaum SR, Carter MJ, Fife CE, DaVanzo J, Haught R, Nusgart M, Cartwright D. An
economic evaluation of the impact, cost, and medicare policy implications of chronic
nonhealing wounds. Value Health. 2018; 21(1): 27-32. doi: 10.1016/j.jval.2017.07.007

3. Singh S, Young A, McNaught CE. The physiology of wound healing. Surgery (Oxford). 2017; 35(9): 473-477. doi: 10.1016/j.mpsur.2017.06.004

4. DesJardins-Park HE, Mascharak S, Chinta MS, Wan DC, Longaker MT. The spectrum
of scarring in craniofacial wound repair. Frontiers in physiology. 2019; 10(322): 1-14.
doi:10.3389/fphys.2019.00322

5. Habiboallah G, Mahdi Z, Majid Z, Nasroallah S, Taghavi AM, Forouzanfar A, Arjmand
N. Enhancement of gingival wound healing by local application of silver nanoparticles
periodontal dressing following surgery: a histological assessment in animal model.
Modern Research in Inflammation. 2014; 3(3): 129. doi:10.4236/mri.2014.33016

6. Durga KN, Bhuvaneswari P, Hemalatha B, Padmalatha K. A review on transdermal drug
delivery system. Asian Journal of Pharmacy and Technology. 2022; 12(2): 159-166.
doi: 10.52711/2231-5713.2022.00027

7. Sailaja AK. An Overall review on topical preparation gel. J Curr Pharma Res. 2014;
4(2): 1138–1143. doi: 10.24018/10.24018/iijmps.2018.v1i1.22

8. Amaliya A, Muhaimina RK, Susanto A, Sutjiatmo AB. Histological assessment of palatal donor
site wound healing after application of moringa oleifera lamarck leaf extract in rats. European
journal of dentistry. 2019; 13(2): 248-254. doi: 10.1055/s-0039-1695065

9. Azevedo ÍM, Araújo-Filho I, Teixeira MMA, Moreira MDF de C, Medeiros AC. Wound healing of diabetic rats treated with moringaoleifera extract. Acta cirúrgica brasileira. 2018; 33(9): 799-805. doi:10.1590/s0102-865020180090000008

10. Poernomo H, Setiawan. The effect of moringa leaf (moringa oleifera) gel on the bleeding
time and collagen density of gingival incision wound healingin marmot (cavia porcellus).
2019; 15(1): 34-39. doi: 10.46862/interdental.v15i1.342

11. Carvalho MTB, Araújo-Filho HG, Barreto AS, Quintans-Júnior LJ, Quintans JSS, Barreto RSS. Wound healing properties of flavonoids: A systematic review highlighting
the mechanisms of action. Phytomedicine. 2021; 90: 153636.
doi: 10.1016/j.phymed.2021.153636

12. Górniak I, Bartoszewski R, Króliczewski J. Comprehensive review of antimicrobial
activities of plant flavonoids. Phytochemistry Reviews. 2018; 18: 241–272.
doi: 10.1007/s11101-018-9591-z

13. Gopalakrishnan A, Ram M, Kumawat S, Tandan SK, Kumar D. Quercetin accelerated
cutaneous wound healing in rats by increasing levels of VEGF and TGF-β1. Growth Factors.
2016; 38(2):187-195. doi: 10.1080/08977194.2020.1822830

14. Pang Y, Zhang Y, Huang L, Xu L, Wang K, Wang D, Guan L, Zhang Y, Yu F, Chen Z, Xie X. Effects and mechanisms of total flavonoids from Blumea balsamifera (L.) DC. on skin wound in rats. Int J Mol Sci. 2017; 18(12): 1–12. doi: 10.3390/ijms18122766

15. Bai R, Yao C, Zhong Z, Ge J, Bai Z, Ye X, Xie T, Xie Y. Discovery of natural antiinflammatory alkaloids: Potential leads for the drug discovery for the treatment of inflammation. Eur J Med Chem. 2021; 213: 113165. doi: 10.1016/j.ejmech.2021.113165

16. Kim YA, Tarahovsky YS, Gaidin SG, Yagolnik EA, Muzafarov EN. Flavonoids determine the
rate of fibrillogenesis and structure of collagen type I fibrils in vitro. Int J Biol Macromol. 2017;
104: 631–637. doi: 10.1016/j.ijbiomac.2017.06.070

17. Lakra R, Kiran MS, Korrapati PS. Collagen scaffold reinforced with furfural for wound healing application. Materials Letters. 2022; 315: 131956. doi: 10.1016/j.matlet.2022.131956

18. Smith PC, Martínez C, Martínez J, McCulloch CA. Role of fibroblast populations in periodontal wound healing and tissue remodeling. Frontiers in physiology. 2019; 10: 270. doi: 10.3389/fphys.2019.00270

19. Suckow MA, Hankenson FC, Wilson RP, Foley PL. The laboratory rat. Academic Press. 2019. 429.



DOI: https://doi.org/10.22146/majkedgiind.76416

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