β-Carotene patch application effects on gingival crevicular fluid volume after panoramic radiography exposure

Anggun Dwi Andini; Rini Widyaningrum; Rurie Ratna Shantiningsih

doi:10.22146/majkedgiind.42486

β-Carotene patch application effects on gingival crevicular fluid volume after panoramic radiography exposure

https://doi.org/10.22146/majkedgiind.42486

Anggun Dwi Andini⁽¹⁾, Rini Widyaningrum⁽²⁾, Rurie Ratna Shantiningsih^(3*)

(1) Dentistry Study Program, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(2) Department of Dentomaxilofacial Radiology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(3) Department of Dentomaxilofacial Radiology, Faculty of Dentistry, Universitas Gadjah Mada, Yogyakarta
(*) Corresponding Author

Abstract

Panoramic radiography is a radiography technique which is commonly used in dentistry. X-ray radiation from panoramic radiography can cause biological damage, such as the increase of gingival crevicular fluid (GCF) volume. One of materials which was known having the ability to decrease the effect of panoramic radiography is β-carotene in mucoadhesive gingival patch form. The application of β-carotene mucoadhesive gingival patch reduce micronucleus increasing in previous study. The aim of this study is to determine the effect of β-carotene mucoadhesive gingival patch application on palatal gingiva of maxillary incisor teeth to the GCF volume of panoramic radiography exposed patients. This study involved 10 subjects (5 females and 5 males). The GCF sample was taken from the labial sulcus of 11, 12 teeth (treatment group) and 21, 22 teeth (control group) of each subject before and after panoramic radiography exposure. β-carotene mucoadhesive gingival patch was applicated on palatal gingiva before panoramic radiography examination. Sample was collected using an absorbent paper which is inserted to the labial gingival sulcus. The GCF on the absorbent papers were marked by adding ninhydrin 2%, then the GCF volume was measured using a sliding caliper. Paired t-test result shows a significant difference between GCF volume in each group before and after panoramic radiography exposure (p<0.05). Independent t-test shows no significant difference between the GCF volume difference in the control and treatment group (p>0.05). The result of this study shows that the application of β-carotene mucoadhesive gingival patch on palatal gingiva of maxillary incisor teeth shows insignificant effect in decreasing the increase of GCF volume in panoramic radiography exposed patients.

Keywords

gingival crevicular fluid; panoramic radiography; β-carotene mucoadhesive gingival patch

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References

1. Whaites E, Drage N. Essentials of dental radiography and radiology, 5th Edition. China: Churchill Livingstone; 2013. 3. 65. 171-175.

2. White SC, Pharoah MJ. Oral radiology principles and interpretation, 7th Edition.
Canada: Mosby Elsevier; 2014. 17; 32-33: 166-167.

3. Arora P, Devi P, Wazir SG. Evaluation of genotoxicity in patients subjected to panoramic radiography by micronucleus assay on epithelial cells of the oral mucosa. J Dent (Tehran). 2014; 11(1): 47-55.

4. Shantiningsih RR, Diba SF. Biological changes after dental panoramic exposure: conventional versus digital. Dent J (Majalah Kedokteran Gigi). 2018; 51(1): 25-28. doi: 10.20473/j.djmkg.v51.i1.p25-28

5. Newman MG, Takei H, Klokkevold PR, Carranza FA. Carranza’s Clinical Periodontology, 12th Edition. Canada: Elsevier Saunders; 2015. 216.

6. Vindani D. Cairan sulkus gingiva dan peranannya dalam bidang kedokteran gigi. Makara Kesehatan. 2007; 1(2): 56-59.

7. Mueller L, Boehm V. Antioxidant activity of β-carotene compounds in different in vitro assays. Molecules. 2011; 16: 1055-1069. doi: 10.3390/molecules16021055

8. Bai SK, Lee SJ, Na HJ, Ha KS, Han JA, Lee H, Kwon YG, Chung CK, Kim YM. Beta-Carotene inhibits inflammatory gene expression in lipopolysaccharide-stimulated macrophages by suppressing redox-based nfkappab activation. Exp Mol Med. 2005; 37(4): 323-334. doi: 10.1038/emm.2005.42

9. Katsuura S, Imamura T, Bando N, Yamanishi R. Beta-Carotene and beta-cryptoxanthin but not lutein evoke redox and immune changes in RAW264 murine macrophages. Mol Nutr Food Res. 2009; 53(11): 1396–1405. doi: 10.1002/mnfr.200800566

10. Shantiningsih RR. Patch gingiva mukoadesif β-carotene sebagai radioprotektor paparan radiografi panoramik (kajian in vivo pada kelinci Galur New Zealand). Fakultas Kedokteran Gigi, Universitas Gadjah Mada, Yogyakarta: Disertasi; 2014. 151-168.

11. Jois HS, Kale AD, Kumar M. Micronucleus as potential biomarker of oral carcinogenesis. IJDA. 2010; 2(2): 197-202.

12. Shantiningsih RR, Diba SF. Efek aplikasi patch gingiva mukoadesif β-carotene akibat paparan radiografi panoramik. Majalah Kedokteran Gigi Indonesia. 2015; 1(2): 186-192. doi: 10.22146/majkedgiind.9121

13. Gilhotra RM, Ikram M, Srivastava S, Gilhotra, N. A Clinical perspective on mucoadhesive buccal drug delivery systems. J Biomed Res. 2014; 28(2): 81-97.doi: 10.7555/JBR.27.20120136

14. Kurniawati L. Kalibrasi spasial citra radiografi dan kalibrasi dosis mesin sinar-x panoramik gigi. Fakultas Matematika dan Ilmu Pengetahuan Alam Universitas Gadjah Mada, Yogyakarta: Tesis; 2013; 12-14.

15. Lee KF, Weng JTYW, Hsu PWC, Chi YH, Chen CK, Liu IY, Chen YC, Wu LS. Gene expression profiling of biological pathway alterations by radiation exposure. Biomed Res Int. 2014; 1-8. doi: 10.1155/2014/834087

16. Wolf HF, Hassell TM. Color atlas of dental hygiene-periodontology. New York: Thieme Stuttgart; 2006. 10-12.

17. Reddy S. Essentials of clinical periodontology and periodontics. New Delhi: Jaypee Brothers Medical Publishers; 2008. 133-134.

18. Nelson SJ. Wheeler’s dental anatomy, Physiology, & Occlusion, 10th Edition. China: Elsevier; 2015. 245 - 249.

19. Barrera G. Oxidative stress and lipid peroxidation products in cancer progression and therapy. ISRN Oncology. 2012; 2012: 1-22. doi: 10.5402/2012/137289

DOI: https://doi.org/10.22146/majkedgiind.42486