Histopathological characteristics of dental socket healing on collagen density following use of pangas catfish (Pangasius djambal) gelatin

Fredy Mardiyantoro; Nenny Prasetyaningrum; Hani Tri Rahmastuti

doi:10.22146/majkedgiind.39830

Histopathological characteristics of dental socket healing on collagen density following use of pangas catfish (Pangasius djambal) gelatin

https://doi.org/10.22146/majkedgiind.39830

Fredy Mardiyantoro^(1*), Nenny Prasetyaningrum⁽²⁾, Hani Tri Rahmastuti⁽³⁾

(1) Department of Oral & Maxillofacial Surgery, Faculty of Dentistry, University of Brawijaya, East Java
(2) Department of Oral Biology, Faculty of Dentistry, University of Brawijaya, East Java
(3) Department of Oral and Maxillofacial Surgery, Faculty of Dentistry, Universitas Brawijaya, East Java
(*) Corresponding Author

Abstract

Tooth extraction is a common treatment in dental practice. However, complications such as bleeding or dry socket may occur during healing process. Pangas catfish (Pangasius djambal) gelatin contains glutamine which could increase the proliferation of fibroblast, accordingly collagen synthesis increases. The study aimed to demonstrate collagen density after the application of pangas catfish gelatin on post-tooth extraction wound of albino rats (Rattus norvegicus). This was
an experimental study with randomized posttest-only control group design which involved male Wistar strain albino rats. The rats were selected using a random sampling method and divided into four groups, namely day-3 control group (n=7),
day-7 control group (n=7), day-3 treatment group (n=7) and day-7 treatment group (n=7). Pangas catfish gelatin was administered into the socket once after extraction. The variable measured in the study was collagen density, determined
by collagen scoring of hematoxylin eosin–stained histopathological preparations. Collagen formation were determined according to the assessment criteria of collagen distribution and density. Data analysis showed there was a significant
difference of collagen synthesis between the control and treatment groups according to Kruskal-Wallis test of which the result of control group day-3 was 1.13 ± 0.34, control group day-7 was 1.76 ± 0.67, treatment group day-3 was 1.66 ±
0.47 and treatment group day-7 was 2.93 ± 0.25. In addition, the day-7 treatment group showed the highest collagen scoring values as compared to the control groups according to the Mann-Whitney test (p<0.05). In conclusion, pangas
catfish gelatin increased collagen density during dental socket healing process after tooth extraction in albino rats.

Keywords

collagen; Pangasius djambal; pangas catfish gelatin; tooth extraction

Full Text:

PDF

References

1. Wang PH, Huang BS, Horng HC, Yeh CC, Chen YJ. Wound healing. J Chinese Med Assoc. 2018; 81(2): 94–101. doi: 10.1016/j.jcma.2017.11.002

2. Böhm S, Strauß C, Stoiber S, Kasper C, Charwat V. Impact of source and manufacturing of collagen matrices on fibroblast cell growth and platelet aggregation. Materials (Basel). 2017; 10(9): 1–14. doi: 10.3390/ma10091086

3. Vieira AE, Repeke CE, De Barros Ferreira S, Colavite PM, Biguetti CC, Oliveira RC, Assis GF, Taga R, Trombone AP, Garlet GP. Intramembranous bone healing process subsequent to tooth extraction in mice: Microcomputed tomography, histomorphometric and molecular characterization. PLoS One. 2015; 10(5): 1–22. doi: 10.1371/journal.pone.0128021

4. Zhou S, Salisbury J, Preedy VR, Emery PW. Increased collagen synthesis rate during wound healing in muscle. PLoS One. 2013; 8(3): 8–11. doi: 10.1371/journal.pone.0058324

5. Nissinen LM, Kähäri VM. Collagen turnover in wound repair - a macrophage connection. J Invest Dermatol. 2015; 135(10): 2350–2352. doi: 10.1038/jid.2015.246

6. Sultana J, Molla MR, Kamal M, Shahidullah M, Begum F, Bashar MA. Histological differences in wound healing in Maxillofacial region in patients with or without risk factors. Bangladesh J Pathol. 2009; 24(1): 3–8. doi: 10.3329/bjpath.v24i1.2874

7. Sun BJ, Chen HJ, Chen Y, An XD, Zhou B Sen. The risk factors of acquiring severe hand, foot, and mouth disease: A meta-analysis. Can J Infect Dis Med Microbiol. 2018; 2018(24): 1-12. doi: 10.1155/2018/2751457

8. Arzt J, Belsham GJ, Lohse L, Bøtner A, Stenfeldt C. Transmission of foot-andmouth disease from persistently Infected Carrier Cattle to Naive Cattle via Transfer of Oropharyngeal Fluid. mSphere. 2018; 3(5): 1–12. doi: 10.1128/msphere.00365-18

9. Nuge T, Hoque ME, Yeow TK, Nordin N, Chowdhury M. Electrospun gelatin composite nanofibres: A review on structural and mechanical characterizations. Regen Res. 2013; 2(2): 39–42.

10. Abdullah MSP, Noordin MI, Mohd Ismail SI, Mustapha NM, Jasamai M, Danik MF, Ahmad WAW, Shamsuddin AF. Recent advances in the use of animal-sourced gelatine as natural polymers for food, cosmetics and pharmaceutical applications. Sains Malaysiana. 2018; 47(2): 323–336. doi: 10.17576/jsm-2018-4702-15

11. Hoque M, Nuge T, Yeow T, Nordin N, Prasad R. Gelatin based scaffolds for tissue engineering-a review. Polym Res J. 2015; 9(1): 15.

12. Ratnasari I, Yuwono SS, Nusyam H, Widjanarko SB. Extraction and characterization of gelatin from different fresh water fishes as alternative sources of gelatin. Int Food Res J. 2013; 20(6): 3085–3091.

13. Li J, Wang Q, Gu Y, Zhu Y, Chen L, Chen Y. Production of composite scaffold containing silk fibroin, chitosan, and gelatin for 3d cell culture and bone tissue regeneration. Med Sci Monit. 2017; 23: 5311–5320. doi: 10.12659/MSM.905085

14. Sunil S, Harsha M. A Modified method for bone regeneration using gelatin sponge with bone graft in treating an osseous defect after root resection. J Heal Sci Res. 2017; 8(2): 80–83. doi: 10.5005/jp-journals-10042-1055

15. Gal P, Kilik R, Mokry M, Vidinsky B, Vasilenko T, Mozes S, Bobrov N, Tomori Z, Bober J, Lenhardt L. Simple method of open skin wound healing model in corticosteroid-treated and diabetic rats: Standardization of semiquantitative and quantitative histological assessments. Vet Med (Praha). 2008; 53(12): 652–659. doi: 10.17221/1973-VETMED

16. Anderson K, Hamm RL. Factors that impair wound healing. J Am Coll Clin Wound Spec. 2012; 4(4): 84–91. doi: 10.1016/j.jccw.2014.03.001

17. Sipahi S, Gungor O, Gunduz M, Cilci M, Demirci MC, Tamer A. The effect of oral supplementation with a combination of betahydroxy-beta-methylbutyrate, arginine and glutamine on wound healing: A retrospective analysis of diabetic haemodialysis patients. BMC Nephrol. 2013; 14(1): 2–7.

18. Chen J, Gao K, Liu S, Wang S, Elango J, Bao B, Dong J, Liu N, Wu W. Fish collagen surgical compress repairing characteristics on wound healing process in vivo. Mar Drugs. 2019; 17(1): 1–12. doi: 10.3390/md17010033

19. Felician FF, Yu RH, Li MZ, Li CJ, Chen HQ, Jiang Y, Tang T, Qi WY, Xu HM. The wound healing potential of collagen peptides derived from the jellyfish Rhopilema esculentum. Chinese J Traumatol - English Ed. 2019; 22(1): 12–20. doi: 10.1016/j.cjtee.2018.10.004

20. Davison-Kotler E, Marshall WS, García-Gareta E. Sources of collagen for biomaterials in skin wound healing. Bioengineering. 2019; 6(3): 56. doi: 10.3390/bioengineering6030056

21. Mardiyantoro F, Munika K, Sutanti V, Cahyati M, Pratiwi AR. Penyembuhan luka rongga mulut. Malang: UB Press; 2018. 1–13.

22. Böhm S, Strauß C, Stoiber S, Kasper C, Charwat V. Impact of source and manufacturing of collagen matrices on fibroblast cell growth and platelet aggregation. Materials (Basel). 2017;10(9). doi: 10.3390/ma10091086

DOI: https://doi.org/10.22146/majkedgiind.39830