Acute Oral Toxicity Test of Antihypertensive Polyherbal Preparations Containing Allium sativum Curcuma aeruginosa & Amomi fructus

https://doi.org/10.22146/farmaseutik.v18i4.79299

Herzan Marjawan(1), Woro Rukmi Pratiwi(2*), Dwi Aris Agung Nugrahaningsih(3), Eti Nurwening Sholikhah(4), Pamungkas Bagus Satriyo(5)

(1) Master Student of Magister of Biomedical Sciences, Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada
(2) departement Pharmacology and Therapy, Faculty of Medicine, Public Health, and Nursing, Universitas Gadjah Mada
(3) Department of Pharmacology, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University
(4) Department of Pharmacology, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University
(5) Department of Pharmacology, Faculty of Medicine, Public Health and Nursing, Gadjah Mada University
(*) Corresponding Author

Abstract


Hypertension is a major factor causing atherosclerotic cardiovascular disease, heart failure, stroke, and kidney failure. Polyherbal preparations containing garlic (Aliium sativum), temu ireng (Curcumae aeruginosae) and cardamom (Amomi fructus) have been widely used to treat hypertension. Despite widely used in community, its safety has not been evaluated. This study aimed to evaluate the single dose oral safety of the polyherbal. The acute oral toxicity test was done using fixed dose methods. Single dose of the polyherbal was administered to female Wistar rats. The clinical examination was done after administration of the polyherbal and continued until the 14th day to check for symptoms of toxicity, changes in body weight. On day 15, the animal was sacrificed and histopathological examination was conducted. The body weight did not differ between animal that received polyherbal and not received polyherbal. However, there was an increase in body weight in a group that received polyherbal at a dose of 2000 mg/kg. The absolute and relative organs weight was also similar among groups. There were no macroscopic and histopathological changes in kidney, liver, heart, spleen, aorta, and lungs. The polyherbal preparations containing Aliium sativum, Curcumae aeruginosae and Amomi fructus is safe with LD50 >2000-5000 mg/kg in Wistar rats.


Keywords


acute oral toxicity test; garlic (Aliium sativum); temu ireng (Curcumae aeruginosae) and cardamom (Amomi fructus); toxicity

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References

Adianti, M., Pramesti, RE, & Puruhito, EF, 2020. Combination Therapy Of Massage And Temu Ireng Herbal ( Curcuma aeoruginosa Roxb .) To Increase Child Appetites And Food Intake. Journal of Vocational Health Studies . 4(1):1-4. URLs https://e-journal.unair.ac.id/JVHS/article/view/21086/11647

Azima, F., 2018. Acute Toxicity Test of Tablets Made from Temulawak in Wistar Male Rats [skirpsi]. Yogyakarta, Gadjah Mada University.

Drug and Food Control Agency of the Republic of Indonesia (BPOM RI) ., 2022, Guidelines for In Vivo Nonclinical Toxicity Testing. Regulation of the Head of the Indonesian Food and Drug Supervisory Agency. Number 7 of 2022. BPOM RI Jakarta.

Baradaran, A, Nasri H ,, & Kopaei MR., 2014, 'Oxidative stress and hypertension: Possibility of hypertension therapy with antioxidants', J res med sci. 19(4):358-367

Choudhury, D., Ghosal, M., Das, AP, & Mandal, P., 2013. Development of single node cutting propagation techniques and evaluation of antioxidant activity of Curcuma aeruginosa Roxburgh rhizome. int. J. Pharm. Pharm. science. 5(2):227-234.

Faria, J., Ahmed, S., Gerritsen, K., Mihaila, SM, & Masereeuw, R., 2019. Kidney-based in-vitro models for drug-induced toxicity testing. Archives of toxicology. 93(12):3397–3418.

Guzik, TJ, & Touyz, RM, 2017. Oxidative Stress, Inflammation and Vascular Aging in Hypertension. Hypertension. 1-9.

Hosseini, A., & Hosseinzadeh, H., 2015. A review on the effects of Allium sativum (Garlic) in metabolic syndrome. Journal of Endocrinological Investigations. 38(11):1147–1157

Khan, RA, Aslam, M., & Ahmed, S., 2016. Evaluation of toxicological profile of a polyherbal formulation. Pharmacology & Pharmacy. 7(1):56-63

Lawal, B., Shittu, OK, Oibiokpa, FI, Mohammed, H., Umar, SI, & Haruna, GM, 2016. Antimicrobial evaluation, acute and sub-acute toxicity studies of Allium sativum. Journal of Acute Diseases. 5(4):296-301.

Nurcholis, W., Khumaida, N., Syukur, M., Bintang, M. & Ardyani, IDAAC, 2015. Phytochemical screening, antioxidant and cytotoxic activities in extracts of different rhizome parts from Curcuma aeruginosa Roxb. int. J. Res. Ayurveda Pharm, 6(5):634-637.

OECD, Test no. 420: acute oral toxicity-fixed dose procedure, OECD Guidelines for the Testing of Chemicals, OECD, Paris, 2001.

Purgiyanti, P., 2015. Effect of Maceration Extract of Temu Hitam (Curcuma aeruginosa Roxb.) on Weight Gain in Male Mice (Mus Musculus). Scientific Journal of Pharmacy, 1(2).

Reanmongkol, W., Subhadhirasakul, S., Khaisombat, N., Fuengnawakit, P., Jantasila, S., & Khamjun, A., 2006. Investigation the antinociceptive, antipyretic and anti-inflammatory activities of Curcuma aeruginosa Roxb. extracts in experimental animals. Songklanakarin J Sci Technol, 28(5):999-1008

Radi, ZA, 2019. Kidney Pathophysiology, Toxicology, and Drug-Induced Injury in Drug Development. International Journal of Toxicology . 38(3):215–227.

Ried, K., 2016. Garlic lowers blood pressure in hypertensive individuals, regulates serum cholesterol, and stimulates immunity: an updated meta-analysis and review. The Journal of nutrition. 146(2):389-396.

Riskesdas., 2018, National Report 2018, Agency for Health Research and Development. Department of Health.

Shittu, OK, Abubakar, AN, & Busari, B., 2015. Toxicological Implications of Methanol Extract from Nigerian Bee Propolis on Some Selected Rat Tissues. J Pharm Biomed Sci. (07):524-531.

Sholikhah, EN, Mustofa, M., Nugrahaningsih, DAA, Yuliani, FS, Purwono, S., Sugiyono, S., Widyarini, S., Ngatidjan, N., Jumina, J., Santosa, D. and Koketsu, M ., 2020. Acute and subchronic oral toxicity study of polyherbal formulation containing Allium sativum L., terminalia bellirica (Gaertn.) roxb., curcuma aeruginosa roxb., and amomum compactum sol. ex. maton in rats. BioMed Research International.

Shouk, R., Abdou, A., Shetty, K., Sarkar, D. & Eid, AH, 2014. Mechanisms underlying the antihypertensive effects of garlic bioactives. Nutrition research. 34(2):106-115.

Smeltzer, SC, & Bare, BG, 2013. Medical surgical nursing. 12th edition. Jakarta :EGC

Wang, HP, Yang, J., Qin, LQ, & Yang, XJ, 2015. Effect of garlic on blood pressure: A meta-analysis. The Journal of Clinical Hypertension. 17(3): 223-231.

WHO, 2014. A Global Brief of Hypertension. Silent killer, global public health crisis.

Wiratama, GA, 2015. Effects of Administration of Chloroform Extract and Essential Oil of Temu Ireng (Curcuma Aeruginosa Roxb.) Rhizome Essential Oil on Histopathological Appearance of Mice Kidney [dissertation]. Surabaya , Airlangga University .

Yudhani, RD, Pesik, RN, Azzahro, S., Anisa, AF, & Hendriyani, R., 2019. Renal Function Parameter on Acute Toxicity Test of Cardamom ( Amomum cardamom ) Seed Extract in Rat. In IOP Conference Series: Materials Science and Engineering (Vol. 578, No. 1, p. 012053).



DOI: https://doi.org/10.22146/farmaseutik.v18i4.79299

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