Preparation of Solid Lipid Nanoparticle-Containing Ovalbumin Based Reverse Micelle-Double Emulsion Technique

Khadijah Zai(1*)

(1) Department of Pharmaceutics, Faculty of Pharmacy, Universitas Gadjah Mada, Yogyakarta, 55281, Indonesia.
(*) Corresponding Author


Encapsulation of protein in the lipid-based nanoparticle is quite challenging. A reverse micelle-double emulsion method could be used for answering this challenge because reverse micelles able to protect the polar core in a nonpolar solvent. Thus, the protein solution can be kept in the interior of the reverse micelle particle and hosted in a lipid matrix of nanoparticles. Herein, we describe the preparation of solid lipid nanoparticles (SLNp) for the encapsulation of ovalbumin (Ova) with reverse micelle-double emulsion. Using several combinations of solid lipids and phospholipids, we prepared SLNp containing Ova-reverse micelle. The optimized formulation enabled the incorporation of Ova in the solid lipid matrix of SLNp with nano size and narrow particle size distribution (PdI), and high entrapment efficiency for Ova. The particle size and zeta potential were measured by a dynamic light scattering spectrophotometer 214.8±15.94 nm and −3.44±0.21 mV, respectively. The entrapment efficiency (EE%) was determined by Spectrofluorometer by determining the quantitative of Ova-FITC labeling was 98.97±0.89%.


Solid lipid nanoparticle; Ovalbumin; Reverse micelle; Double emulsion

Full Text:



Müller, R.H., Maaßen, S., Weyhers, H., Specht, F., Lucks, J.S. 1996, Cytotoxicity of magnetite loaded polylactide, polylactide/glycolide particles and solid lipid nanoparticles (SLN), Int J Pharm, 138, 85–94.

Müller, D., Rühl, S., Runge, K., Schulze-Forster, M., Wolfgang. 1997, Cytotoxicity of solid lipid nanoparticles as a function of the lipid matrix and the surfactant, Pharm Res, 14, 458–462.

Almeida, A.J., Runge, S., Müller, R.H. 1997, Peptide-loaded solid lipid nanoparticles (SLN): influence of production parameters, Int J Pharm,149, 255–265.

Khadijah, Z., Kazuki, Y., Akihiro, K., Takeshi, M., Yoshiki, K. 2018, Preparation of complexes between ovalbumin nanoparticles and retinoic acid for efficient induction of tolerogenic dendritic cells, Anal Sci, 34, 1243-1248.

Vierros, S. and Sammalkorpi, M. 2015, Phosphatidylcholine reverse micelles on the wrong track in molecular dynamics simulations of phospholipids in an organic solvent, J Chem Phys, 142, 094902.

Shinji, S., Sosaku, I., Yoh, S., Mitsutoshi, N., Xin, Q.L., Minoru, S., and Shintaro, F. 2001, Formation and Characterization of Reversed Micelles Composed of Phospholipids and Fatty Acids, J Colloid Interface Sci, 240, 566–572.

Ma, F., Huang, H.Y., Zhou, L., Yang, C., Zhou, J.H., Liu, Z.M. 2012, Study on the conformation changes of Lysozyme induced by Hypocrellin A: the mechanism investigation. Spectrochim, Acta A Mol Biomol Spectrosc, 97, 1159–1165.

Krimm, S. and Bandekar, J. 1986, Vibrational spectroscopy and conformation of peptides, polypeptides, and proteins, Adv Protein Chem, 38, 181-364.


Article Metrics

Abstract views : 283 | views : 243


Majalah Farmaseutik Indexed by:

Creative Commons Licence
This work is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 4.0 International License.