Aktivitas Antimikroba Ekstrak Etil Asetat Fungi Endofit Eutypa linearis

Baiq Maylinda Gemantari; Fitra Romadhonsyah; Arief Nurrochmad; Subagus Wahyuono; Puji Astuti

doi:10.22146/farmaseutik.v18i3.64133

Aktivitas Antimikroba Ekstrak Etil Asetat Fungi Endofit Eutypa linearis

https://doi.org/10.22146/farmaseutik.v18i3.64133

Baiq Maylinda Gemantari⁽¹⁾, Fitra Romadhonsyah⁽²⁾, Arief Nurrochmad⁽³⁾, Subagus Wahyuono⁽⁴⁾, Puji Astuti^(5*)

(1) Fakultas Farmasi, Universitas Gadjah Mada Departemen Farmasi, Fakultas Kesehatan, Universitas Hamzanwadi
(2) Faculty of Pharmacy, Universitas Gadjah Mada
(3) Pharmacology and Clinical Pharmacy Department, Faculty of Pharmacy, Universitas Gadjah Mada
(4) Pharmaceutical Biology Department, Faculty of Pharmacy, Universitas Gadjah Mada
(5) Pharmaceutical Biology Department, Faculty of Pharmacy, Universitas Gadjah Mada
(*) Corresponding Author

Abstract

Fungi endofit banyak dilaporkan mampu memproduksi senyawa metabolit yang memiliki beragam aktivitas biologis. Diisolasi dari salah satu tanaman berkhasiat Indonesia yang dikenal dengan nama tanaman jinten (Coleus amboinicus Lour.), pada penelitian ini dilakukan observasi aktivitas antimikroba fungi endofit Eutypa linearis. Ekstrak etil asetat dari media fermentasi E.linearis dalam beragam konsentrasi diujikan kepada beberapa mikroba bakteri dan fungi yakni Staphyloccocus aureus, Pseudomonas aeruginosa, Bacillus subtilis, dan Candida albicans. Aktivitas antimikroba dievaluasi menggunakan metode mikrodilusi pada media Mueller Hinton Broth untuk kultur bakteri dan Brain Heart Infussion Broth untuk kultur fungi. Nilai optical density pada panjang gelombang 600 nm yang diperoleh dari penggunaan microplate reader digunakan untuk menghitung nilai IC₅₀ ekstrak. Hasil penelitian menunjukkan nilai IC₅₀ ekstrak etil asetat media fermentasi E.linearis pada S.aureus adalah sebesar 260,69 µg/mL; P.aeruginosa 359,32 µg/mL; B.subtilis 163,74 µg/mL; dan C.albicans 92,75 µg/mL. Berdasarkan metode bioautografi kontak, diketahui senyawa aktif sebagai antimikroba tersebut merupakan kelompok senyawa yang polar.

Keywords

fungi endofit; antibakteri; antifungi; mikrodilusi; bioautografi

Full Text:

PDF

References

Allison, D., & Gilbert, P. (2004). Bacteria. In Hugo and Russell’s Pharmaceutical Microbiology (pp. 23–43). John Wiley & Sons, Ltd. https://doi.org/10.1002/9780470988329.ch3

Arumugam, G., Swamy, M. K., & Sinniah, U. R. (2016). Plectranthus amboinicus (Lour.) Spreng: Botanical, Phytochemical, Pharmacological and Nutritional Significance. Molecules (Basel, Switzerland), 21(4), 369. https://doi.org/10.3390/molecules21040369

Astuti, P., Rollando, R., Pratoko, D. K., Wahyuono, S., & Nurrochmad, A. (2021). Antimicrobial and Cytotoxic Activities of A compound Produced by An Endophytic Fungus Isolated from The Leaves of Coleus amboinicus Lour. International Journal of Pharmaceutical Research, 13(01), 2632–2644. https://doi.org/10.31838/ijpr/2021.13.01.394

Astuti, P., Rollando, R., Wahyuono, S., & Nurrochmad, A. (2020). Antimicrobial activities of isoprene compounds produced by an endophytic fungus isolated from the leaves of Coleus amboinicus Lour. Journal of Pharmacy & Pharmacognosy Research, 8(4), 280–289.

Balouiri, M., Sadiki, M., & Ibnsouda, S. K. (2016). Methods for in vitro evaluating antimicrobial activity: A review. Journal of Pharmaceutical Analysis, 6(2), 71–79. https://doi.org/10.1016/j.jpha.2015.11.005

Bhatt, P., & Negi, P. S. (2012). Antioxidant and Antibacterial Activities in the Leaf Extracts of Indian Borage (Plectranthus amboinicus). Food and Nutrition Sciences, 3(2), 146–152. https://doi.org/10.4236/fns.2012.32022

Bnyan, I. A., Abid, A. T., & Obied, H. N. (2014). Antibacterial Activity of Carvacrol against Different Types of Bacteria. Journal of Natural Sciences Research, 4(9), 13–16.

Chen, L., Zhang, Q.-Y., Jia, M., Ming, Q.-L., Yue, W., Rahman, K., Qin, L.-P., & Han, T. (2016). Endophytic fungi with antitumor activities: Their occurrence and anticancer compounds. Critical Reviews in Microbiology, 42(3), 454–473. https://doi.org/10.3109/1040841X.2014.959892

de Lira Mota, K., de Oliveira Pereira, F., de Oliveira, W., Lima, I., & de Oliveira Lima, E. (2012). Antifungal Activity of Thymus vulgaris L. Essential Oil and Its Constituent Phytochemicals against Rhizopus oryzae: Interaction with Ergosterol. Molecules, 17(12), 14418–14433. https://doi.org/10.3390/molecules171214418

DeBaggio, T., & Tucker, A. O. (2009). The Encyclopedia of Herbs: A Comprehensive Reference to Herbs of Flavor and Fragrance. Timber Press.

Erny Sabrina, M. N., Razali, M., Mirfat, A. H. S., & Mohd Shukri, M. A. (2014). Antimicrobial activity and bioactive evaluation of Plectranthus amboinicus essential oil. American Journal of Research Communication, 2(12), 121–127.

Farkas, A., Pap, B., Kondorosi, É., & Maróti, G. (2018). Antimicrobial Activity of NCR Plant Peptides Strongly Depends on the Test Assays. Frontiers in Microbiology, 9. https://doi.org/10.3389/fmicb.2018.02600

Gemantari, B. M. (2021). Karakterisasi dan Skrining Potensi Akktivitas Fungi Endofit Eutypa linearis dari Daun Tanaman Jinten (Coleus amboinicus Lour.) [Tesis]. Universitas Gadjah Mada.

Gemantari, B. M., Romadhonsyah, F., Nurrochmad, A., Wahyuono, S., & Astuti, P. (2021). Bioactivity Screening of Endophytic Fungus Eutypa linearis isolated from Coleus amboinicus (Lour.). Indonesian Journal of Pharmacy, 32, 86–95. https://doi.org/10.22146/ijp.1077

Hasibuan, P. A. Z. H., Sitorus, P., Satria, D., & Sibuea, R. D. (2019). Antioxidant Properties and Cytotoxic Activity of Ethyl Acetate Fraction of Plectranthus amboinicus (Lour.) Spreng. Leaves on HeLa and T47D Cell Lines. Indonesian Journal of Cancer Chemoprevention, 10(1), 37–45.

Jork, H. (Ed.). (1990). Thin-layer chromatography: Reagents and detection methods. VCH.

Kumar, P., Singh, B., Thakur, V., Thakur, A., Thakur, N., Pandey, D., & Chand, D. (2019). Hyper-production of taxol from Aspergillus fumigatus, an endophytic fungus isolated from Taxus sp. Of the Northern Himalayan region. Biotechnology Reports, 24. https://doi.org/10.1016/j.btre.2019.e00395

Laila, F., Fardiaz, D., Yuliana, N. D., Damanik, M. R. M., & Nur Annisa Dewi, F. (2020). Methanol Extract of Coleus amboinicus (Lour) Exhibited Antiproliferative Activity and Induced Programmed Cell Death in Colon Cancer Cell WiDr. International Journal of Food Science, 2020, e9068326. https://doi.org/10.1155/2020/9068326

Marino, M., Bersani, C., & Comi, G. (1999). Antimicrobial activity of the essential oils of Thymus vulgaris L. measured using a bioimpedometric method. Journal of Food Protection, 62(9), 1017–1023. https://doi.org/10.4315/0362-028x-62.9.1017

Marston, A. (2011). Thin-layer chromatography with biological detection in phytochemistry. Journal of Chromatography A, 1218(19), 2676–2683. https://doi.org/10.1016/j.chroma.2010.12.068

Pina-Vaz, C., Gonçalves Rodrigues, A., Pinto, E., Costa-de-Oliveira, S., Tavares, C., Salgueiro, L., Cavaleiro, C., Gonçalves, M. J., & Martinez-de-Oliveira, J. (2004). Antifungal activity of Thymus oils and their major compounds. Journal of the European Academy of Dermatology and Venereology: JEADV, 18(1), 73–78. https://doi.org/10.1111/j.1468-3083.2004.00886.x

Porras-Alfaro, A., & Bayman, P. (2011). Hidden Fungi, Emergent Properties: Endophytes and Microbiomes. Annual Review of Phytopathology, 49(1), 291–315. https://doi.org/10.1146/annurev-phyto-080508-081831

Rodrigues, F. F. G., Costa, J. G. M., Rodrigues, F. F. G., & Campos, A. R. (2013). Study of the Interference between Plectranthus Species Essential Oils from Brazil and Aminoglycosides. Evidence-Based Complementary and Alternative Medicine, 2013, e724161. https://doi.org/10.1155/2013/724161

Sakunpak, A., & Sueree, L. (2018). Thin-layer chromatography–contact bioautography as a tool for bioassay-guided isolation of anti- Streptococcus mutans compounds from Pinus merkusii heartwood. Journal of Planar Chromatography, 31(5), 355–359. https://doi.org/10.1556/1006.2018.31.5.2

Santovito, E., das Neves, J., Greco, D., D’Ascanio, V., Sarmento, B., Logrieco, A. F., & Avantaggiato, G. (2018). Antimicrobial properties of rosin acids-loaded nanoparticles against antibiotic-sensitive and antibiotic-resistant foodborne pathogens. Artificial Cells, Nanomedicine, and Biotechnology, 46(sup3), S414–S422. https://doi.org/10.1080/21691401.2018.1496924

Sivaranjani, D., Saranraj, P., Manigandan, M., & Amala, K. (2019). Antimicrobial activity of Plectranthus amboinicus solvent extracts against Human Pathogenic Bacteria and Fungi. Journal of Drug Delivery and Therapeutics, 9(3), 36–39. https://doi.org/10.22270/jddt.v9i3.2604

Strobel, G. A. (2003). Endophytes as sources of bioactive products. Microbes and Infection, 5(6), 535–544.

Swamy, M. K., Arumugam, G., Kaur, R., Ghasemzadeh, A., Yusoff, M. Mohd., & Sinniah, U. R. (2017). GC-MS Based Metabolite Profiling, Antioxidant and Antimicrobial Properties of Different Solvent Extracts of Malaysian Plectranthus amboinicus Leaves. Evidence-Based Complementary and Alternative Medicine : ECAM, 2017. https://doi.org/10.1155/2017/1517683

DOI: https://doi.org/10.22146/farmaseutik.v18i3.64133