SITOTOKSISITAS SENYAWA HASIL ISOLASI DAUN TITHONIA DIVERSIFOLIA (HEMSLEY) A. GRAY) TERHADAP SEL T47D, MCF7 DAN EVSA-T



Arfian Bela Mahardika(1*), Subagus Wahyuono(2), Mae Sri Hartanti Wahyuningsih(3),

(1) Fakultas Farmasi, Universitas Gadjah Mada
(2) Fakultas Farmasi, Universitas Gadjah Mada
(3) Fakultas Farmasi, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Tanaman kembang bulan (Tithonia diversifolia (Hemsley) A. Gray) merupakan salah satu tanaman yang secara tradisional digunakan masyarakat untuk mengobati berbagai penyakit. Penelitian awal menggunakan metode Bioassay Guided Isolation telah diperoleh senyawa aktif (MTT sel HeLa; IC50 9,776 g/mL). Efek sitotoksik senyawa aktif terhadap sel kanker payudara belum diketahui. Penelitian ini bertujuan untuk mengetahui sitotoksisitas senyawa aktif hasil isolasi daun T. diversifolia terhadap sel kanker payudara T47D, MCF-7 dan EVSA-T dengan menghitung nilai IC50-nya. Senyawa hasil isolasi daun T. diversifolia diuji aktivitas sitotoksiknya terhadap sel kanker payudara T47D, MCF-7 dan EVSA-T menggunakan metode MTT dengan seri kadar 0,39-50 µg/mL. Setiap kelompok kadar dilakukan triplikat dan dihitung persentase penghambatan pertumbuhan sel T47D, MCF-7 dan EVSA-T dengan analisis probit untuk menghitung IC50-nya. Hasil uji menunjukkan bahwa isolat aktif memiliki efek sitotoksik terhadap sel T47D, MCF-7 dan EVSA-T dengan nilai IC50 berturut-turut adalah (2,44; 4,697; 3,522) μg/mL, sehingga potensial dikembangkan sebagai agen antikanker khususnya kanker payudara.

Keywords


Tithonia diversifolia; sitotoksisitas; sel kanker payudara, MTT;

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