SITOTOXICITY OF COMPOUND ISOLATED FROM THE LEAVES OF TITHONIA DIVERSIFOLIA (HEMSLEY) A.GRAY) AGAINST T47D, MCF-7 AND EVSA-T CELLS

https://doi.org/10.22146/farmaseutik.v12i2.26455

Arfian Bela Mahardika(1*), Subagus Wahyuono(2), Mae Sri Hartanti Wahyuningsih(3)

(1) Fakultas Farmasi, Universitas Gadjah Mada
(2) Fakultas Farmasi, Universitas Gadjah Mada
(3) Fakultas Farmasi, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Tithonia diversifolia (Hemsley) A. Gray) is one of the plants that are traditionally used to treat various diseases. The previous studies using Bioassay Guided Isolation methods has obtained the active compound (MTT, HeLa cells; IC50 9.776 g / mL). The cytotoxic effects of active compounds against breast cancer cells is unknown. This study aims to determine the cytotoxicity of theactive compound isolated from the leaves of T. diversifolia on breast cancer cells (T47D; MCF-7 and EVSA-T). The isolated compounds from T. diversifolia tested their cytotoxicity against breast cancer cells (T47D, MCF-7 and EVSA-T), using MTT method with a series of concentration of 0.39 to 50 ug/mL. Each concentration of the group performed triplicate and calculated the percentage of cell growth inhibition with probit analysis to calculate the IC50 value. The test results showed that the active compound have a cytotoxic effect on T47D, MCF-7 and EVSA-T cells with IC50 values are respectively (2.44; 4.697; 3.522) ug/mL, so this compound potential to be developed as an anticancer agent, especially breastcancer


Keywords


Tithonia diversifolia, cytotoxicity, breast cancer cells, MTT

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References

Amundson, S.A., Myers, T.G., Scudiero, D., Kitada, S., Reed, J.C., and Fornace, A.J., 2000, An Informatics Approach Identifying Markers of Chemosensitivity in Human Cancer Cell Lines, Cancer Res, 60:6010-6110 Anonim, 2007, ATCC Cell Biology, available from http://www.atcc.org/common/catalog/numSearch/numResults.cfm?atcc Num=HTB-22, cited in 25 June 2007. Anonim,2012,from http://winners4lifeindonesia. com/transfer-factor-untuk-penyakit- kanker - payudara. pdf. Akses Juni 2016. Anonim 2016, from (https://www.dsmz.de/catalogues/details/culture/ACC-433.html). Akses Juli 2016 Aouali, N., Morjani, H., Trussardi, A., Soma, E., Giroux, B., and Manfait, M., 2003, Enhanced Cytotoxicity and Nuclear Accumulation of Doxorubicin-loaded Nanospheres in Human Breast Cancer MCF-7 Cells Expressing MRP1, International Journal of Oncology, 23:1195-1201. Benzivin, C. Devehat. Tomasi, S. Boustie, J., 2003. Cytotoxic Activities of some Lischen Extracts on Murine and Humasn Cancer Cell Lines. Phytomedicine 10:499-503. Burger, A., 1970, Medicinal Chemistry, 3th ed., p. 681 - 694, Wiley Interscience Publication, New York. Butt, A.J., Firth, S.M., King, M.A., and Baxter, R.C., 2000, Insulin-Like Growth Factor-Binding Protein-3 Modulates Expression of Bax and Bcl-2 and Potentiates P53-Independent Radiation-Induced Apoptosis In Human Breast Cancer Cells, J. Biol Chem, 275 (50) : 39174 – 39181. Freshney, I.R. 2000. Culture of Animal Cells Manual of Basic Technique. New York : A John Wiley and Sons Ltd. Goffin, E., Ziemons, E., De Mol, P., de Madureira Mdo, C., Martins, AP., da Cunha, AP., Philippe, G., Tits, M., Angenot, L., and Frederich, M., 2002, In vitro antiplasmodial activity of Tithonia diversifolia anf identification of its main active constituent:Tagitinin C, Planta Medica, 68(6);543-545. Gu, J. Q., Gills, JJ., Park, EJ., Mata-Greenwood, E., Hawthorne, ME., Axelrod, F., Chavez, PI., Fong, HH., Mehta, RG., Pezzuto, JM. and Kinghorn, AD., 2002, Sesquiterpenoids from Tithonia diversifolia with potencial care chemopreventive activity, J Nat Prod, 65(4),532-536. Mahardika, A.B., 2010, Efek Sitotoksik Senyawa Hasil Isolasi Daun Kembang Bulan (Tithonia diversifolia (Hemsley) A. Gray) terhadap Sel Kanker Payudara T47D [Skripsi], Fakultas Farmasi, UGM Mardihusodo, HR., Wahyuningsih, MSH., Astuti I., 2013, The effect of active compound isolated from the leaves of Kembang bulan (T. diversifolia (Hemsley) A. Gray) on cell cycle and angiogenesis of WiDR cell line, Journal of the Medical Sciences, 45 (3), 101-111. Medina, D., Kittrell, F. S., 2003. p35 function is required for hormone – mediated protein of mouse mamary tumorigenesis. Canc Res. 63: 6140-6143. Menchetner, E., Kyshtoobayeva, A., Zonis, S., Kim, H., Stroup, R., Garcia, R., Parker, R.J., and Fruehauf, J.P., 1998, Levels of Multidrug Resistance (MDR1) P-Glycoprotein Expression by Human Breast Cancer Correlate with in Vitro Resistance to Taxol and Doxorubicin, Clinical Cancer Research, 4:389-398. Mosman,T., 1983. Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assay. J Immunol Methods 65,55-63. Nabin, C., Baruah., Ram P, Sharma., Madhusudanan, K.P., and Go palakrishna, T., 1979, Sesquiterpene lactone of Tithonia diversifolia. Stereochemistry of the Tagitinins and related compounds, J. Org.Chem, 44(11);1831. Nafrialdi dan Gan, S., 1995, Dalam farmakologi dan Terapi, Edisi IV, Ganiswara, S.G., Bagian Farmakologi Fakultas Kedokteran UI, jakarta, 48, 702 Nooter K, 1997, the Role Of P53 In Resistance To Cytotoxic Therapy Of Human Cancer. South West Cancer News. 4, 12-13 Nooter K, Burger H, Schenk P, Stoter G, 1999, Molecular mechanisms of drug resistance and sensitivity. Oncological Research at the Erasmus University Rotterdam-University Hospital Rotterdam, 39-40 Onuki, R., Kawasaki, H., Baba, T., dan Taira, K., 2003, Analysis of A Mitochondrial Apoptotic Pathway Using Bid-Targeted Ribozymes in Human MCF7 Cells in the Absence of A Caspase-3-Dependent Pathway, Antisense and Nucleic Acid Drug Development, 13 (2): 75-82. Prunet, C., Lemaire-Ewing, S., Ménétrier, F., Néel, D., dan Lizard, G., 2005, Activation of Caspase-3-Dependent and -Independent Pathways During 7-Ketocholesterol- and 7β-Hydroxycholesterol-Induced Cell Death: A Morphological and Biochemical Study, Journal of Biochemical and Molecular Toxicology, 19 (5): 311-326. Sieuwerts, A. M., Klijn, J. G. M., Peters, H. A., and Foekens, J. A., 1995, The MTT Tetrazolium salt assay scrutinized: How to use this assay reliably to measure metabolic activity of cell cultures in vitro for the assessment of growth characteristics, IC50-values and cell survival, Eur. J. Clin. Chem. Clin. Biochem., 33, 813-823. Suffness, M., dan Pezzuto, J.M., 1990, Assay Related to Cancer Drug Discovery. In: K. Hostettmann (Ed.): Methods in Plant Biochemistry. Vol.6. Assays for Bioactivity. Academic Press. London. pp: 71-133. Wahyuningsih MSH., Syarif RA., Rakhmawati R., 2008. Isolasi Senyawa Berpotensi Antikanker dari Fraksi Aktif Tithonia diversifolia (Hemsley) A. Gray dan Penelusuran Mekanisme Apoptosisnya [Laporan Penelitian Dana Masyarakat], Fakultas Kedokteran UGM Wahyuningsih MSH., Syarif RA., Suharmi S., Murini T., Saputra F., Adiguno Suryo W., 2013, Selectivity of Purified Extract from the leaves of Tithonia diversifolia (Hemsley) A.Gray) against Hela Cells, Trad. Med. J., 18(1), 22-28 Wahyuningsih, MSH., Wijayanti MA., Budiyanto A., Muhammad Hanafi, 2015, Isolation and Identification of Potential Cytotoxic Compound from Kembang bulan [Tithonia diversifolia (Hemsley) A. Gray] Leaves, Int. J. Pharm. Pharm. Sci. 7 (6), 298-301.



DOI: https://doi.org/10.22146/farmaseutik.v12i2.26455

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