First Report of Cladosporium dominicanum Zalar, de Hoog & Gunde-Cim. Infecting Whitefly on Ornamental Plants in Bali, Indonesia

https://doi.org/10.22146/jtbb.86865

I Putu Sudiarta(1*), Dwi Sugiarta(2), Dewa Gede Wiryangga Selangga(3), Gusti Ngurah Alit Susanta Wirya(4), I Wayan Diksa Gargita(5), Ketut Ayu Yuliadhi(6), Katrina Klett(7)

(1) 
(2) Master of Agricultural Biotechnology, Faculty of Agriculture, Udayana University. Postgraduate Building, Panglima Besar Sudirman Street, Denpasar, 80234, Bali, Indonesia
(3) Bachelor of Agroecotechnology, Faculty of Agriculture, Udayana University. Agroecotechnology Building, Panglima Besar Sudirman Street Denpasar, 80234, Bali, Indonesia
(4) Master of Agricultural Biotechnology, Faculty of Agriculture, Udayana University. Postgraduate Building, Panglima Besar Sudirman Street, Denpasar, 80234, Bali, Indonesia; Bachelor of Agroecotechnology, Faculty of Agriculture, Udayana University. Agroecotechnology Building, Panglima Besar Sudirman Street Denpasar, 80234, Bali, Indonesia
(5) Bachelor of Agroecotechnology, Faculty of Agriculture, Udayana University. Agroecotechnology Building, Panglima Besar Sudirman Street Denpasar, 80234, Bali, Indonesia
(6) Bachelor of Agroecotechnology, Faculty of Agriculture, Udayana University. Agroecotechnology Building, Panglima Besar Sudirman Street Denpasar, 80234, Bali, Indonesia
(7) Department of Natural Resources Scientific Management Program, University of Minnesota, USA. 115 Green Hall 1530 Cleveland Ave. N. St. Paul, MN 55108
(*) Corresponding Author

Abstract


As natural enemies, entomopathogenic fungi are essential for controlling certain plant pests, such as whitefly. Many types of entomopathogenic fungi can infect whiteflies, including those from the genera Aschersonia, Metarhizium, Beauveria, and Cladosporium. Currently, there is great interest in using entomopathogenic fungi as an environmentally friendly pest control in organic and sustainable agricultural systems. This study aimed to identify entomopathogenic fungus associated with whiteflies. Identification of entomopathogenic fungus was performed morphologically and molecularly using DNA barcoding with ITS-1 and ITS-4 as primers, in addition to sequencing and phylogenetic tree analysis. Whiteflies infected with entomopathogenic fungus were discovered on ornamental plants (Premna serratifolia, Ficus religiosa, and Ficus rumphii) in Denpasar, Bali, Indonesia from January to March 2023. We found that samples from the field had mycelium that was unbranched or sparingly branched with solitary conidiophores arising terminally from ascending hyphae or laterally from plagiotropous hyphae. Samples from PDA media had ramoconidia with darkened septa, straight and conidia in long branched chains, branching in all directions, conidia were obovoid, ovoid to limoniform. The fungal colonies had the characteristics of being grey-olivaceous, olivaceous-black, and the margins were grey-livaceous to white, gray olivaceous in colony center due to abundant sporulation. In addition, based on the BLAST nucleotide sequence from NCBI GenBank, the insect pathogen phylogenetic tree found in Denpasar, Bali, is in the same clade as Cladosporium dominicanum strain SCAU014, accession number KY827344.1. Based on morphological and molecular analysis the fungus that infects whiteflies on ornamental plants is C. dominicanum.


Keywords


Biological control; Entomopathogenic; Molecular; Morphology; Natural enemies

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References

Abdel-Baky, N.F. & Abdel-Salam, A.H., 2003. Natural incidence of Cladosporium spp. as a bio-control agent against whiteflies and aphids in Egypt. Journal of Applied Entomology, 127(4), pp.228–235. doi: 10.1046/j.1439-0418.2003.00662.x.

Alfiky, A., 2022. Screening and Identification of Indigenous Entomopathogenic Fungal Isolates from Agricultural Farmland Soils in Nile Delta, Egypt. Journal of Fungi, 8(1), 54. doi: 10.3390/jof8010054.

Ayu, D.K. et al., 2021. Resilience response to yellow leaf curl disease and identification of resistance gene analogs (RGA) in pepper (capsicum annuum). Biodiversitas, 22(11), pp.4731–4739. doi: 10.13057/biodiv/d221104.

Bajwa, U. & Sandhu, K.S., 2014. Effect of handling and processing on pesticide residues in food - A review. Journal of Food Science and Technology, 51(2), pp.201–220. doi: 10.1007/s13197-011-0499-5.

Bensch, K. et al., 2018. Cladosporium species in indoor environments. Studies in Mycology, 89, pp.177–301. doi: 10.1016/j.simyco.2018.03.002.

Bhosale, D.N. et al., 2020. Efficacy of aschersonia aleyrodis against citrus blackfly and whitefly. Indian Journal of Entomology, 82(3), pp.464–466. doi: 10.5958/0974-8172.2020.00120.0.

Ghanim, M., 2014. A review of the mechanisms and components that determine the transmission efficiency of Tomato yellow leaf curl virus (Geminiviridae; Begomovirus) by its whitefly vector. Virus Research, 186, pp.47–54. doi: 10.1016/j.virusres.2014.01.022.

Ghiaie, A.I. et al., 2017. Molecular characterization of environmental Cladosporium species isolated from Iran. Current Medical Mycology, 3(1), pp.1–5. doi: 10.18869/acadpub.cmm.3.1.1.

Hidayat, P. et al., 2018. Species, Host Range, and Identification Key of Whiteflies of Bogor and Surrounding Area. Journal of Tropical Plant Pests and Diseases, 18(2), pp.127–150. doi: 10.23960/j.hptt.218127-150.

Islam, M.T., 2022. Current Status and Future Prospects of Cladosporium sp., a Biocontrol Agent for Sustainable Plant Protection. Biocontrol Science, 27(4), pp.185–191. doi: 10.4265/bio.27.185.

Islam, T. et al., 2019. Identification and application of a fungal biocontrol agent Cladosporium cladosporioides against Bemisia tabaci. Biotechnology and Biotechnological Equipment, 33(1), pp.1698–1705. doi: 10.1080/13102818.2019.1695541.

Kumar, K.K. et al., 2019. Microbial biopesticides for insect pest management in India: Current status and future prospects. Journal of Invertebrate Pathology, 165, pp.74–81. doi: 10.1016/j.jip.2018.10.008.

Lee, M.-H. et al., 2014. Effect of Cyantraniliprole against of Bemisia tabaci and Prevention of Tomato Yellow Leaf Curl Virus (TYLCV). The Korean Journal of Pesticide Science, 18(1), pp.33–40. doi: 10.7585/kjps.2014.18.1.33.

Lovett, B. & St. Leger, R.J., 2017. The Insect Pathogens. Microbiology Spectrum, 5(2). doi: 10.1128/microbiolspec.funk-0001-2016.

McGuire, A.V. & Northfield, T.D., 2020. Tropical Occurrence and Agricultural Importance of Beauveria bassiana and Metarhizium anisopliae. Frontiers in Sustainable Food Systems, 4, 6. doi: 10.3389/fsufs.2020.00006.

Mrosso, S.E., Ndakidemi, P.A. & Mbega, E.R., 2023. Farmers’ Knowledge on Whitefly Populousness among Tomato Insect Pests and Their Management Options in Tomato in Tanzania. Horticulturae, 9(2), 253. doi: 10.3390/horticulturae9020253.

Nurbaya, S. et al., 2022. Morphological and molecular identification of mealybugs on mangosteen fruits in registered packaging houses on the Bali Island. International Journal of Agricultural Technology, 18(6), pp.2497–2508.

Paradza, V.M. et al., 2021. Virulence and horizontal transmission of Metarhizium anisopliae by the adults of the greenhouse whitefly Trialeurodes vaporariorum (Hemiptera: Aleyrodidae) and the efficacy of oil formulations against its nymphs. Heliyon, 7(11), e08277. doi: 10.1016/j.heliyon.2021.e08277.

Paradza, V.M. et al., 2022. Efficacy of Metarhizium anisopliae and (E)–2–hexenal combination using autodissemination technology for the management of the adult greenhouse whitefly, Trialeurodes vaporariorum Westwood (Hemiptera: Aleyrodidae). Frontiers in Insect Science, 2, 991336. doi: 10.3389/finsc.2022.991336.

Prayogo, Y. & Bayu, M.S.Y.I., 2020. Biological control of Bemisia tabaci gennadius by using entomopathogenic fungi Aschersonia aleyrodis. IOP Conference Series: Earth and Environmental Science, 456, 012041. doi: 10.1088/1755-1315/456/1/012041.

Selangga, D.G.W. et al., 2022. Determinants of symptom variation of Pepper yellow leaf curl Indonesia virus in bell pepper and its spread by Bemisia tabaci. Biodiversitas, 24(2), pp.869–877. doi: 10.13057/biodiv/d240224.

Sudiarta, I.P., Suputra, I.P.W. & Wirya, G.N.A.S., 2019. New Report of Insect Pathogenic Fungi (Aschersonia sp.) of Citrus Whitefly (Dialeurodes sp.) in Bali Indonesia. Research in: Agricultural & Veterinary Sciences, 3(1), pp.22–27.

Suputra, I.P.W. et al., 2019. New Report of Distribution of Entomopathogenic Fungi Aschersonia sp. Infected Whitefly in Bali. AGROTROP, 9(2), pp.188–196. doi: 10.24843/AJoAS.2019.v09.i02.p10.

Syafitri, D.D., Fauzana, H. & Salbiah, D., 2017. Abundance of Aphids and Mealybugs The Citrus Plant of Siam (Citrus nobilis Lour.) in The Village of Kuok, Kuok District, Kampar Regency, Riau Province. JOM FAPERTA UNRI, 4(1), pp.1–11.

Tamura, K. et al., 2013. MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. Molecular Biology and Evolution, 30(12), pp.2725–2729. doi: 10.1093/molbev/mst197.

Temaja, I.G.R M. et al., 2022. Relationship between viruliferous Bemisia tabaci population and disease incidence of Pepper yellow leaf curl Indonesia virus in chili pepper. BIODIVERSITAS, 23(10), pp.5360–5366. doi: 10.13057/biodiv/d231046.

Thompson, J.D. et al., 1997. The CLUSTAL X windows interface: Flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Research, 25(24), pp.4876–4882. doi: 10.1093/nar/25.24.4876.

Tripathi, Y.N. et al., 2019. Biopesticides: Current status and future prospects in India. In Bioeconomy for Sustainable Development. Springer Singapore, pp.79–109. doi: 10.1007/978-981-13-9431-7_6.

Wang, C. & Feng, M.-G., 2014. Advances in fundamental and applied studies in China of fungal biocontrol agents for use against arthropod pests. Biological Control, 68(1), pp.129–135. doi: 10.1016/j.biocontrol.2013.06.017.

Wang, C. & Wang, S. 2017.Insect Pathogenic Fungi: Genomics, Molecular Interactions, and Genetic Improvements. Annual Review of Entomology, 62, pp.73–90. doi: 10.1146/annurev-ento-031616-035509.

Zafar, J. et al., 2016. Effectiveness of Beauveria bassiana Against Cotton Whitefly, Bemisia tabaci (Gennadius) (Aleyrodidae: Homoptera) on Different Host Plants. Pakistan J. Zool, 48(1), pp.91–99.

Zalar, P. et al., 2007. Phylogeny and ecology of the ubiquitous saprobe Cladosporium sphaerospermum, with descriptions of seven new species from hypersaline environments. Studies in Mycology, 58, pp.157–183. doi: 10.3114/sim.2007.58.06.



DOI: https://doi.org/10.22146/jtbb.86865

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