In Vitro Culture of Phalaenopsis amabilis (L.) Blume Orchid for Seedling Production with Banana Extract Supplementation and Light Treatment for Ex Situ Conservation

https://doi.org/10.22146/jtbb.70868

Dyah Ayu Puspita Arum(1), Endang Semiarti(2*)

(1) Departement of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55282, Special Region of Yogyakarta, Indonesia
(2) Departement of Tropical Biology, Faculty of Biology, Universitas Gadjah Mada, Jl. Teknika Selatan, Sekip Utara, Sleman, 55282, Special Region of Yogyakarta, Indonesia
(*) Corresponding Author

Abstract


In vitro culture is one of the effective cultivation methods for seedling production of orchids that can be used as a powerful tool in the conversation of orchids. The aims of the present study were to (i) investigate the effects of the addition of banana extract (0 g/l, 100 g/l, and 150 g/l) on media (NP media), in two different light regimes (light and dark conditions) on the growth of plantlets of an epiphytic orchid Phalaenopsis amabilis in in vitro culture. Methods used included (i) subculturing orchid seedlings in treatments media, (ii) measuring leaves and roots chlorophyll content and growth parameters, (iii) anatomical preparation of leaves and roots of the seedlings. The results showed that the best condition for getting greater seedlings of P. amabilis plantlets is in media with an addition of 100 g/L banana extract in light condition. The highest amount of chlorophyll in the P. amabilis leaves was found in medium with the addition of 100 g/L banana extract medium in light conditions. The thickness of mesophyll and the largest root diameter of P. amabilis seedlings were also found in media with the addition of 100 g/L banana extract medium in light condition. In conclusion, the addition of 100gr/L banana extract into basic culture medium will be beneficial for seedlings production of P. amabilis with great appearance, for ex situ orchid conservation programs.


Keywords


anatomy, banana extract, Phalaenopsis amabilis, orchid, in vitro.

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References

Arditti, J., 2008. Micropopagation of Orchid Second Edition, Oxford: Blackwell Publishing.

Arimarsetiowati, R., & Ardiyani, F., 2012. The effects of shooting and rooting of arabica coffee propagation through Embryogenesis Somatic auxin uses). Pelita Perkebunan (a Coffee and Cocoa Research Journal), 28(2), pp.82-90.

Armarego-Marriott, T., Sandoval-Ibañez, O. & Kowalewska, Ł., 2020. Beyond the darkness: recent lessons from etiolation and de-etiolation studies. Journal of experimental botany, 71(4), pp.1215-1225. doi: 10.1093/jxb/erz496

Beck, C.B., 2010. An Introduction to Plant Structure and Development. New York: Cambridge University Press

Bercu, R., Bavaru, A. & Broască, L., 2011. Anatomical Aspects of P. amabilis (L.) Blume, Annals of RSCB, pp. 103-109.

Berlyn, G.P. & Miksche, J.P., 1976. Botanical microtechnique and cytochemistry, Ames: Lowa State University Press.

Cortleven, A. & Schmülling, T., 2015. Regulation of chloroplast development and function by cytokinin. Journal of experimental botany, 66(16), pp.4999-5013. doi: 10.1093/jxb/erv132

Djajanegara, I., 2010. Pemanfaatan limbah buah pisang dan air kelapa sebagai bahan media kultur jaringan anggrek bulan (Phalaenopsis amabilis) tipe 229. Jurnal Teknologi Lingkungan, 11(3), pp.373-380. doi: 10.29122/jtl.v11i3.1182

Filho, A.C.A.C. et al., 2017. Influence of Potassium Levels on Root Growth and Nutrient Uptake of Upland Rice Cultivars. Revista Caatinga, 30, pp.32-44. doi: 10.1590/1983-21252017v30n104rc

Hapsari, L. & Lestari, D. A., 2016. Fruit characteristic and nutrient values of four Indonesian banana cultivars (Musa spp.) at different genomic groups. AGRIVITA: Journal of Agricultural Science, 38(3), pp.303-311. doi: 10.17503/agrivita.v38i3.696

Harborne, J.B., 1998. Phytochemical Methods, a Guide to Modern Techniques of Plant Analysis. London: Chapman and Hall.

Haris, A. & Mercuriani, I.S., 2018. Pertumbuhan Anggrek Rhynchostylis retusa pada Medium Kultur In Vitro dengan Penambahan Jus Buah Pisang. Biologi-S1, 7(6), pp.367-381.

Hasanah, U. & Suwarsi, E., 2014. Pemanfaatan Pupuk Daun, Air Kelapa dan Bubur Pisang sebagai Komponen Medium Pertumbuhan Plantlet Anggrek Dendrobium Kelemense. Biosaintifika: Journal of Biology & Biology Education, 6(2), pp.137-144. doi: 10.15294/biosaintifika.v6i2.3784

Heydari, M.M., Brook, R.M. & Jones, D. L., 2019. The role of phosphorus sources on root diameter, root length and root dry matter of barley (Hordeum vulgare L.). Journal of plant nutrition, 42(1), pp.1-15. doi: 10.1080/01904167.2018.1509996

Joca, T.A.C. et al., 2017. The velamen of epiphytic orchids: variation in structure and correlations with nutrient absorption. Flora, 230, pp.66-74. doi: 10.1016/j.flora.2017.03.009.

Manzur, J.P., Calvache-Asensio, M.D.L.N. & Rodriguez-Burruezo, A., 2014. Growth regulators and darkness increase efficiency in in vitro culture of immature embryos from peppers. Scientia Agricola, 71, pp.488-493. doi: 10.1590/0103-9016-2013-0230

McCauley, A., Jones, C. & Jacobsen, J., 2009. Plant Nutrition Function and Deficiency and toxicity Symptoms. Bozeman: Montana State University

Metusala, D. et al., 2017. Comparative leaf and root anatomy of two Dendrobium species (Orchidaceae) from different habitat in relation to their potential adaptation to drought. AIP Conference Proceedings, 1862. doi: 10.1063/1.4991222

Monteuuis, O. & Bon, M. C., 2000. Influence of auxins and darkness on in vitro rooting of micropropagated shoots from mature and juvenile Acacia mangium. Plant cell, tissue and organ culture, 63(3), pp.173-177. doi: 10.1023/A:1010611126950

Moore, T.C., 1989. Biochemistry and Physiology of Plant Hormone Ed-2. New York: Springer-Verhag.

Moreira, F.P.A.S. et al., 2013. Structural adaptations of two sympatric epiphytic orchids (Orchidaceae) to a cloudy forest environment in rocky outcrops of Southeast Brazil. Revista de Biología Tropical, 61(3), pp. 1053-1065.

Muthukumar, T., & Shenbagam, M., 2018. Vegetative anatomy of the orchid Bulbophyllum sterile (Orchidaceae: Epidendroideae). Lankesteriana, 18(1), pp.13-22. doi: 10.15517/lank.v18i1.32701

Nurfadilah, S., Yulia, N.D., & Ariyanti, E.E., 2016. Morphology, anatomy, and mycorrhizal fungi colonization in roots of epiphytic orchids of Sempu Island, East Java, Indonesia. Biodiversitas Journal of Biological Diversity, 17(2). doi: 10.13057/biodiv/d170229

Paiva, É.A.S., et al. 2003. The influence of light intensity on anatomical structure and pigment contents of Tradescantia pallida (Rose) Hunt. cv. purpurea Boom (Commelinaceae) leaves. Brazilian Archives of Biology and Technology, 46, pp.617-624. doi:10.1590/S1516-89132003000400017

Pazil, S.N., 2009. Perbandingan Aktivitas Antioksidan Ekstrak Daging Pisang Raja (Musa AAB ‘Pisang Raja’) Dengan Vitamin A, Vitamin C, dan Katekin Melalui Penghitungan Bilangan Peroksida, Srkipsi, Fakultas Kedokteran, Universitas Indonesia

Pradhan, B.M. & Bajracharya, D.M., 2020. Anatomical Study of Dendrobium (Orchidaceae) of Nepal. Annals of Plant Sciences, 9(7), pp.3916-3948. doi:10.21746/aps.2020.9.7.1

Qomariyah, F.L., & Dewanti, P., 2019. Pertumbuhan Planlet Anggrek Dendrobium sp. pada Media Tahap III secara In-Vitro. Jurnal Ilmiah Inovasi, 19(1), pp.13-16. doi: 10.25047/jii.v19i1.853

Rindyastuti, R. et al., 2018. Leaf anatomical characters of four epiphytic orchids of Sempu Island, East Java, Indonesia: The importance in identification and ecological adaptation. Biodiversitas Journal of Biological Diversity, 19(5), pp.1906-1918. doi: 10.13057/biodiv/d190543

Rukmana, R., 2000. Anggrek Bulan, Yogyakarta: Penerbit Kanisius.

Sallolo, S.T., Sadimantara, I.G.R. & Wijayanto, T., 2012. Pertumbuhan anggrek Dendrobium Candy Stripe Lasianthera pada media sapih Vacin dan Went secara in vitro dengan penambahan ekstrak pisang raja dan fish emulsion. J. Penelitian Agronomi, 1(1), pp.57-62.

Semiarti, E. et al., 2007. Agrobacterium-mediated transformation of the wild orchid species Phalaenopsis amabilis. Plant Biotechnology, 24(3), pp.265-272. doi: 10.5511/plantbiotechnology.24.265

Semiarti, E. et al., 2010. High-frequency genetic transformation of Phalaenopsis amabilis orchid using tomato extract-enriched medium for the pre-culture of protocorms. The Journal of Horticultural Science and Biotechnology, 85(30), pp.205-210. doi: 10.1080/14620316.2010.11512655

Setiari, N. et al., 2018. Micropropagation of Dendrobium phalaenopsis orchid through overexpression of embryo gene AtRKD4. AGRIVITA, Journal of Agricultural Science, 40(2), pp.284-294. doi: 10.17503/agrivita.v40i2.1690

Silva Júnior, J.M.D. et al., 2013. Changes in anatomy and chlorophyll synthesis in orchids propagated in vitro in the presence of urea. Acta Scientiarum: Agronomy, 35(1), pp.65-72. doi:10.4025/actasciagron.v35i1.15356

Simpsons, M.G., 2019. Plant Anatomy and Physiology. In: M.G. Simpson, (eds) Plant Systematic Third Edition. New York : Academic Press.

Souza, G.R.B. et al., 2013. Pulp fruit added to culture medium for in vitro orchid development. Semina: Ciências Agrárias, 34(3), pp.1141-1146.

Theng, P.A. & Korpenwar, A.N., 2014. Phytochemical, Phermacognostic and Physiocochemical evaluation of endangered terrestrial orchid Geodorum densiflorum (Lam.) Schltr. IJSR, 3(9), pp.1250-1253.

Tränkner, M., Tavakol, E. & Jákli, B., 2018. Functioning of potassium and magnesium in photosynthesis, photosynthate translocation and photoprotection. Physiologia plantarum, 163(3), pp. 414-431. doi: 10.1111/ppl.12747

Utami, E.S.W., Harianto, S. & Manuhara, Y.S.W., 2016. Pengaruh Pemberian Ekstrak Pisang pada Media VW terhadap Induksi Akar dan Pertumbuhan Tunas Dendrobium lasianthera JJ Sm. Jurnal Agrotop, 6(1), 35-42.

van Gelderen, K., Kang, C. & Pierik, R., 2018. Light signaling, root development, and plasticity. Plant physiology, 176(2), pp. 1049-1060. doi: 10.1104/pp.17.01079

Xu, X. et al., 2020. Effects of potassium levels on plant growth, accumulation and distribution of carbon, and nitrate metabolism in apple dwarf rootstock seedlings. Frontiers in Plant Science, 11, pp. 904. doi: 10.3389/fpls.2020.00904

Xu, J. et al., 2021. Light regulation of horticultural crop nutrient and utilization. Horticultural Plant Journal, 7(5), pp.367-379. doi: 10.1016/j.hpj.2021.01.005

Yulianti, Y., Aisyah, S. I. & Sukma, D., 2016. Pengaruh Bahan Organik Nabati dan Hewani terhadap pertumbuhan protocorm like bodies Phalaenopsis amabilis (L.) blume. Jurnal Hortikultura Indonesia, 7(3), pp. 176-186. doi: 10.29244/jhi.7.3.176-186

Yuniardi, F., 2019. Aplikasi Dimmer Switch pada Rak Kultur Sebagai Pengatur Kebutuhan Intesitas Cahaya Optimum Bagi Tanaman In Vitro. Indonesian Journal of Laboratory, 1(4), pp. 8-13.

Zheng, L. & Van Labeke, M. C., 2017. Long-term effects of red-and blue-light emitting diodes on leaf anatomy and photosynthetic efficiency of three ornamental pot plants. Frontiers in plant science, 8, pp. 917. doi: 10.3389/fpls.2017.00917



DOI: https://doi.org/10.22146/jtbb.70868

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