* Corresponding Author
How to link: Plasmid Curing and Lead Tolerance Ability of Pediococcus pentosaceus
https://doi.org/10.22146/jtbb.68615 Fadhil Neamah Al-Kanany(1*), Raghad Shubbar Jaafar(2)
(1) Biological Development Department, Marine Science Center, University of Basrah, Basrah, Iraq, 61001
(2) Biological Development Department, Marine Science Center, University of Basrah, Basrah, Iraq, 61001
(*) Corresponding Author
Abstract
Pediococcus pentosaceus has a high level of resistance to heavy metals, making it one of the biological alternatives for dealing with heavy metal contamination in the environment. The current study sought to identify the genetic factors responsible for this ability by curing the plasmid of these bacteria using various curing agents (Acridine orange and Sodium Dodecyl Sulfate). The findings demonstrate that both curing agents had perfect curing ability. The bacteria were able to tolerate a wide range of Lead concentrations (50-2000 ppm). This capacity was reduced when the plasmid was removed, but it did not disappear, implying additional resistance genes on the chromosomes. The antibiotic susceptibility observations supported the significance of plasmid genes in lead resistance ability, the findings revealed differences in the pattern of antibiotic resistance between wild and cure plasmid bacteria, the wild one had different antibiotic MIC values for Nitrofurantoin and Trimethoprim/Sulfamethoxazole (<=16 and <=10 µg/ml) respectively), on the other hand for the same antibiotics, the MIC results for plasmid-cured bacteria were 64 and 80 g/ml. Based on the findings, we can conclude that plasmid genes play a significant role in Pediococcus pentosaceus to resist lead, and there is a strong correlation between antibiotic resistance and lead resistance.
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Ainsworth, S. et al., 2014. The Lactococcus lactis plasmidome: much learnt, yet still lots to discover. FEMS Microbiology Reviews, 38(5), pp.1066–1088. doi: 10.1111/1574-6976.12074. Alegre, M.T., Rodríguez, M.C. & Mesas, J.M., 2005. Nucleotide sequence, structural organization and host range of pRS4, a small cryptic Pediococcus pentosaceus plasmid that contains two cassettes commonly found in other lactic acid bacteria. FEMS Microbiology Letters, 250(1), pp.151–156. doi: 10.1016/j.femsle.2005.07.003. Axelsson, L., 2004. Lactic acid bacteria: Classification and physiology. In Lactic Acid Bacteria Microbiological and Functional Aspects, Third Edition: Revised and Expanded. pp. 1–66. Bergey, D.H., 1994. Bergey’s manual of determinative bacteriology, Lippincott Williams & Wilkins. Carattoli, A., 2003. Plasmid-Mediated Antimicrobial Resistance in Salmonella enterica. Current Issues in Molecular Biology, 5(4), pp.113–122. doi: 10.21775/cimb.005.113. Cui, Y. et al., 2012. Class IIa Bacteriocins: Diversity and New Developments. International Journal of Molecular Sciences, 13(12), pp.16668–16707. doi: 10.3390/ijms131216668. Cui, Y. et al., 2015. Plasmids from food lactic acid bacteria: Diversity, similarity, and new developments. International Journal of Molecular Sciences, 16(6), pp.13172–13202. doi: 10.3390/ijms160613172. GG, I. & OH, S., 2016. Role of Plasmid-Borne Genes in the Biodegradation of Polycyclic Aromatic Hydrocarbons (PAHs) by Consortium of Aerobic Heterotrophic Bacteria. Journal of Petroleum & Environmental Biotechnology, 07(01), p.264. doi: 10.4172/2157-7463.1000264. Gonzalez, C.F. & Kunka, B.S., 1986. Evidence for Plasmid Linkage of Raffinose Utilization and Associated α-Galactosidase and Sucrose Hydrolase Activity in Pediococcus pentosaceus. Applied and Environmental Microbiology, 51(1), pp.105–109. doi: 10.1128/aem.51.1.105-109.1986. Haque, S.A.M., 2017. Plasmid profile analysis and curing of plasmids in Enterobacteriaceae isolated from patients of Urinary Tract infection. Jaafar, R.S., 2020. Bioremediation of lead and cadmium and the strive role of pediococcus pentosaceus probiotic. Iraqi Journal of Veterinary Sciences, 34(1), pp.51–57. doi: 10.33899/ijvs.2019.125581.1092. Marzan, L.W. et al., 2017. Isolation and biochemical characterization of heavy-metal resistant bacteria from tannery effluent in Chittagong city, Bangladesh: Bioremediation viewpoint. The Egyptian Journal of Aquatic Research, 43(1), pp.65–74. doi: https://doi.org/10.1016/j.ejar.2016.11.002. Mills, S. et al., 2006. Plasmids of lactococci – genetic accessories or genetic necessities? FEMS Microbiology Reviews, 30(2), pp.243–273. doi: 10.1111/j.1574-6976.2005.00011.x. Miyoshi, T., Iwatsuki, T. & Naganuma, T., 2005. Phylogenetic Characterization of 16S rRNA Gene Clones from Deep-Groundwater Microorganisms That Pass through 0.2-Micrometer-Pore-Size Filters. Applied and Environmental Microbiology, 71(2), pp.1084–1088. doi: 10.1128/AEM.71.2.1084-1088.2005. Qin, X. et al., 2012. Complete genome sequence of Enterococcus faecium strain TX16 and comparative genomic analysis of Enterococcus faecium genomes. BMC Microbiology, 12(1), p.135. doi: 10.1186/1471-2180-12-135. Raghad, J., Amin, A.S. & Asaad, A.T., 2016. Bioaccumulation of cadmium and lead by Shewanella oneidensis isolated from soil in Basra governorate, Iraq. African Journal of Microbiology Research, 10(12), pp.370–375. doi: 10.5897/ajmr2016.7912. Rozwandowicz, M. et al., 2018. Plasmids carrying antimicrobial resistance genes in Enterobacteriaceae. Journal of Antimicrobial Chemotherapy, 73(5), pp.1121–1137. doi: 10.1093/jac/dkx488. Samanta, A. et al., 2012. An investigation on heavy metal tolerance and antibiotic resistance properties of bacterial strain Bacillus sp . isolated from municipal waste. Journal of Microbiology and Biotechnology Research, 2(1), pp.178–189. Schroeter, J. & Klaenhammer, T., 2009. Genomics of lactic acid bacteria. FEMS Microbiology Letters, 292(1), pp.1–6. doi: 10.1111/j.1574-6968.2008.01442.x. Sobolev, D. & Begonia, M.F.T., 2008. Effects of heavy metal contamination upon soil microbes: lead-induced changes in general and denitrifying microbial communities as evidenced by molecular markers. International journal of environmental research and public health, 5(5), pp.450–456. doi: 10.3390/ijerph5050450. Sulochana M.B., Arunasri R, P.A.. and M.R.K., 2014. Strain Improvement and Characterization of Lipase Produced by Pseudomonas Sp . Research Article. In Cell science & Molecular Biology. pp. 6–10. Tankovic, J., Leclercq, R. & Duval, J., 1993. Antimicrobial susceptibility of Pediococcus spp. and genetic basis of macrolide resistance in Pediococcus acidilactici HM3020. Antimicrobial Agents and Chemotherapy, 37(4), pp.789–792. doi: 10.1128/AAC.37.4.789. Teresa Alegre, M., Carmen Rodríguez, M. & Mesas, J.M., 2009. Characterization of pRS5: A theta-type plasmid found in a strain of Pediococcus pentosaceus isolated from wine that can be used to generate cloning vectors for lactic acid bacteria. Plasmid, 61(2), pp.130–134. doi: https://doi.org/10.1016/j.plasmid.2008.10.002. Wegrzyn, G. & Wegrzyn, A., 2002. Stress responses and replication of plasmids in bacterial cells. Microbial Cell Factories, 1(1), p.2. doi: 10.1186/1475-2859-1-2.
DOI: https://doi.org/10.22146/jtbb.68615
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