Using Feathers for Molecular Sexing of Straw-headed Bulbul (Pycnonotus zeylanicus) Offsprings

Pramana Yuda(1*), Worawidh Wajjwalku(2)

(1) Faculty of Tehcnobiology, Universitas Atma Jaya Yogyakarta
(2) Akkharatchakumari Veterinary College, Walailak University, Thasala, Nakhonsithammarat
(*) Corresponding Author


Sex determination of of straw-headed bulbul offspring was carried out from 27 offspring’s plucked feather samples in a captive breeding program. Using direct PCR, this study provided more evidences that feather samples are reliable as a source of DNA for non-invasive and effective molecular sexing. The study also revealed that the offspring sex ratio of straw-headed bulbul was slightly inclined towards males, but there was no significant difference from the value of 0.5.  



dPCR; feather; molecular sexing; non-invasive sampling; straw-headed Bulbul

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