Genetic Diversity of Dicranopteris and Sticherus from Rokan Hulu, Riau Based on ISSR Marker

Afni Atika Marpaung(1*), Ratna Susandarini(2)

(1) Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(2) Faculty of Biology, Universitas Gadjah Mada. Jl. Teknika Selatan, Sekip Utara, Yogyakarta 55281, Indonesia
(*) Corresponding Author


Genetic diversity of eleven taxa consisted of Dicranoperis speciosa, D. curanii, and D. linearis with seven varieties and Sticherus truncatus with two varieties from Rokan Hulu, Riau was analyzed using ISSR markers generated from 10 primers. Nine out of ten ISSR primers produced a high level of polymorphism, with six of them showed 100% polymorphism. The genetic similarity was calculated using Jaccard's similarity coefficient, and cluster analysis using the Unweighted Pair-Group Method with Arithmetic Mean. The result showed that the genetic similarity of the eleven taxa under study ranged from 0.377 to 0.627 indicated a moderate level of genetic diversity and the clusters did not separate Dicranopteris from Sticherus.



Dicranopteris; Pteridophyte; Sticherus.; molecular marker; taxonomy

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Adjie, B. & Lestari, WS., 2011. Flora Indonesiana: Ferns of Bali. Bali Botanic Garden, Indonesian Institute of Sciences, Baturiti, Tabanan, Bali, Indonesia.

Andayani N. et al., 2016. Keragaman genetik inbrida jagung QPM dan Provit-A berdasarkan marka SSRs (Simple Sequence Repeats). Penelitian Pertanian Tanaman Pangan, 35(2), pp.133-140. DOI:

Am, A.D. et al., 2014. Study of genetic diversity among Philodendron varieties by Randomly Amplified Polymorphic DNA Markers. Journal of Ornamental Plants, 4(1) pp.19-26.

Animasaun, D.A. et al., 2018. Polymorphism and genetic diversity assessment of some ornamental ferns by microsatellite (ISSR) markers. Journal of Applied Horticulture, 20 (3) pp. 1-5. DOI: 10.37855/jah.2018.v20i03.41

Arini, DID. & Kinho, J., 2012. Keragaman jenis tumbuhan paku (Pteridophyta) di Cagar Alam Gunung Ambang Sulawesi Utara. Info BPK Manado, 2 (1) p. 18.

Arnaud-Haond S. et al., 2005. Assessing genetic diversity in clonal organisms: low diversity or low resolution? Combining power and cost efficiency in selecting markers. Journal of Heredity, 96 (4) pp. 434–440.

Cichorz, S. et al., 2014. Miscanthus: Genetic diversity and genotype identification using ISSR and RAPD Markers. Mol Biotechnol, 56 (10) pp. 911–924. DOI: 10.1007/s12033-014-9770-0

Ellstrand, NC. & Elam, DR., 1993. Population genetic consequences of small population size: Implications for plant conservation. Annual Review Ecology and Systematics, 24 (1) pp. 217–242.

Fernando, DD. et al., 2015. Genetic analysis of the threatened American hart's-tongue fern (Asplenium scolopendrium var. americanum [Fernald] Kartesz and Gandhi): Insights into its mating system and implications for conservation. Biochemical Systematics and Ecology, 62 pp. 25-35.

Go, R. et al., 2012. The Distribution of the ferns Gleicheniaceae in Peninsular Malaysia. Acta Biologica Malaysiana, 1 (1) pp. 18-25. DOI:10.7593/abm/1.1.18

Guowen, XYZ. & Fufang Li, 2011. Clonal diversity and genetic differentiation of endangered liana species Monimopetalum chinense (Celastraceae) in Poyang Lake Basin. Procedia Environmental Sciences, 10, pp. 2453–2459. DOI: 10.1016/j.proenv.2011.09.382

Holttum, RE., 1957. Florae Malesianae Praecursores XVI. on The Taxonomic Subdivision of the Gleicheniaceae, with Description of New Malaysian Species and Varieties. Reinwardtia, 4 pp. 257–280.

João, C. Teixeira & Christian D. Huber, 2021. The inflated significance of neutral genetic diversity in conservation genetics. Proceedings of the National Academy of Sciences, 118 (10), pp. e2015096118

Konan, NO, et al., 2020. Genetic diversity of exotic and local eggplants (Solanum spp.) cultivated in Côte d’Ivoire based on ISSR markers. Biodiversitas, 21 (8). pp. 3651-3658.

Korpelainen, H. et al., 2005. Four tropical, closely related fern species belonging to the genus Adiantum L. are genetically distinct as revealed by ISSR fingerprinting. Genetica, 125( 2-3), pp. 283–291, DOI: 10.1007/s10709-005-0747-6

Kovach, WL. 2007. Mutivariate Statistical Package (MVSP) Plus Version 3.22 User’s Manual. Publish by Kovach Computing Services.

Lambertini, C. et al., 2010. Research article Genetic diversity in three invasive clonal aquatic species in New Zealand. BMC Genetics, 11 (52). pp. 1-18. doi: 10.1186/1471-2156-11-52

Mekbib, Y. et al., 2020. The level of genetic diversity and differentiation of tropical lotus, Nelumbo nucifera Gaertn. (Nelumbonaceae) from Australia, India, and Thailand. Bot Stud, 61 (15). pp. 1-11, doi: 10.1186/s40529-020-00293-3

Rahayu, Sri E. et al., 2007. Genetic diversity of Pandanus and freycinetia from Java Based on ISSR Marker. Floribunda, 3 (4), pp. 95-103. DOI:

Santos, MP. et al., 2020. The genetic diversity andpopulation structure of two endemic Amazonian quillwort (IsoetesL.) species. PeerJ8:e10274, doi: 10.7717/peerj.10274

Smith, A.R. et al., 2006. A classification for extant ferns. Taxon, 55 (3). pp. 705-731,

Tryon, RM. & Tryon, AF. 1982. Ferns and Allied Plants: With Special Reference to Tropical America. Springer-Verlag. New York. Sofiyanti, N. et al., 2020. Jenis-Jenis tumbuhan paku di Pulau Rangsang, Kepulauan Meranti, Riau dan karakteristik morfologi-palinologi. Jurnal Biologi Tropis, 20(1). pp. 102-110, DOI:

Russell, AE. et al., 1999. Pattern of clonal diversity in Dicranopteris linearis on Mauna Loa, Hawaii. Biotrpioca, 31(3). pp. 449–459,

Van Steenis, CGGJ. 1959. Flora Malesiana: Series II Volume 1 Pteridophyta. Martinus Nijhoff. Dr W. Junk Publishers The Hague. Boston. London

Vidyashree, SY. et al., 2019. Analysis of Genetic Diversity of Ferns of Western Ghats in Mudigere region of Karnataka based on ISSR Markers. Int.J.Curr.Microbiol.App.Sci, 8(2). pp. 1372-1378, DOI:10.20546/IJCMAS.2019.802.161

Wang, T. et al., 2012. Population genetic variation in the tree fern Alsophila spinulosa (Cyatheaceae): Effects of reproductive strategy. PLOS ONE, 7 (7). pp. e41780,

Yang, L. et al., 2021. Rethinking the ecosystem functions of Dicranopteris, a widespread genus of ferns. Frontiers in Plant Science, 11 pp. 581513, DOI: 10.3389/fpls.2020.581513

Yatskievych, G., 2018. Gleicheniaceae. Encyclopædia Britannica, inc. Accessed on April 28, 2020.

Zietkiewicz E. et al., 1994. Genome fingerprinting by simple sequence repeat (SSR)-anchored polymerase chain reaction amplification. Genomics, 20 (12). pp.176–183, doi: 10.1006/geno.1994.1151.


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