Detection of Knockdown-Resistance Mutations (V1016G and F1534C) in Dengue Vector from Urban Park, Surabaya, Indonesia
Shifa Fauziyah(1), Sri Subekti(2*), Budi Utomo(3), Teguh Hari Sucipto(4), Hebert Adrianto(5), Aryati Aryati(6), Puspa Wardhani(7), Soegeng Soegijanto(8)
(1) Institute of Tropical Disease, Universitas Airlangga, Mulyorejo Street, Mulyorejo 60115, Surabaya, East Java, Indonesia
(2) Faculty of Fisheries and Marine Science, Universitas Airlangga, Mulyorejo, 60115, Surabaya, East Java, Indonesia Entomology Study Group, Institute of Tropical Disease, Universitas Airlangga, Mulyorejo, 60115, Surabaya, East Java, Indonesia
(3) Department of Public Health and Preventive Medicine, Faculty of Medicine, Universitas Airlangga, Prof. Dr. Moestopo Street, Tambaksari 60132, Surabaya, East Java, Indonesia
(4) Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Mulyorejo Street, Mulyorejo 60115, Surabaya, East Java, Indonesia
(5) School of Medicine, Universitas Ciputra, CitraLand CBD Boulevard, Sambikerep 60219, Surabaya, East Java, Indonesia
(6) Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Mulyorejo Street, Mulyorejo 60115, Surabaya, East Java, Indonesia Clinical Pathology Department, Faculty of Medicine, Universitas Airlangga, Prof.Dr.Moestopo Street, Tambaksari 60132, Surabaya, East Java, Indonesia
(7) Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Mulyorejo Street, Mulyorejo 60115, Surabaya, East Java, Indonesia Clinical Pathology Department, Faculty of Medicine, Universitas Airlangga, Prof.Dr.Moestopo Street, Tambaksari 60132, Surabaya, East Java, Indonesia
(8) Dengue Study Group, Institute of Tropical Disease, Universitas Airlangga, Mulyorejo Street, Mulyorejo 60115, Surabaya, East Java, Indonesia
(*) Corresponding Author
Abstract
An urban park is potentially a source of vector-borne disease transmission due to it being a natural and artificial mosquito breeding habitats combined with people's continuous presence. Thus, this study aims to screen the occurrence of knockdown-resistance (kdr) mutant alleles (V1016G and F1534C) in mosquito populations collected from urban parks in Surabaya, Indonesia. Cross sectional study was conducted in July 2019. A total of 28 ovitraps were installed in seven urban parks, having four ovitraps installed in each park. In total, 1,662 eggs were collected, and only 187 emerged into adult mosquitoes, consisting of 97 Aedes (Stegomyia) aegypti and 90 Aedes (Stegomyia) albopictus. All-female adult mosquitoes (n=55) were tested using allele-specific polymerase chain reaction assay (AS-PCR) to detect voltage gated sodium channel (VGSC) gene mutations. This study found no mutations in Valine to Glysine mutation in point 1016 (V1016G) and Phenylalanine to Cysteine in point 1534 (F1534C) alleles in both two species. All of mosquito samples have wild type genotype of kdr alleles (V1016V and F1534F). Data were analysed using R Studio 1.4 Version by Genetics package. Results showed that the frequency of resistant alleles (G1016 and C1534) was zero, and the frequency of susceptible allele was 1 (V1016 and F1534). Insecticide bioassay could not be established due to the limited number of adult mosquitoes, so insecticide resistance status could not be determined. However, this study can be used as preliminary monitoring for the vector control program.
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Amelia-Yap, Z.H. et al., 2018. Pyrethroid resistance in the dengue vector Aedes aegypti in Southeast Asia: Present situation and prospects for management. Parasites and Vectors, 11(1), p.17.
Amelia-Yap, Z.H. et al., 2019. V1016G Point Mutation: The Key Mutation in the Voltage-Gated Sodium Channel (Vgsc) Gene of Pyrethroid-Resistant Aedes aegypti (Diptera: Culicidae) in Indonesia. Journal of Medical Entomology, 56(4), pp.953–958.
Bowman, L.R., Donegan, S. & McCall, P.J., 2016. Is Dengue Vector Control Deficient in Effectiveness or Evidence ?: Systematic Review and Meta-analysis. PLoS Neglected Tropical Diseases, 10(03), e004551.
Brengues, C. et al., 2003. Pyrethroid and DDT cross-resistance in Aedes aegypti is correlated with novel mutations in the voltage-gated sodium channel gene. Medical and Veterinary Entomology, 17(1), pp.87–94.
Chang, C. et al., 2009. A novel amino acid substitution in a voltage-gated sodium channel is associated with knockdown resistance to permethrin in Aedes aegypti. Insect Biochemistry and Molecular Biology, 39(4), pp.272–278.
Corbel, V. & Guessan, R.N., 2013. Distribution , Mechanisms , Impact and Management of Insecticide Resistance in Malaria Vectors : A Pragmatic Review. Springer.
David, J., Gallet, C. & Despre, L., 2007. The evolutionary ecology of insect resistance to plant chemicals. TRENDS in Ecology and Evolution, 22(6), pp.298–307.
Djouaka, R. F. et al., 2008. Expression of the cytochrome P450s, CYP6P3 and CYP6M2 are significantly elevated in multiple pyrethroid resistant populations of Anopheles gambiae s.s. from Southern Benin and Nigeria. BMC Genomics, 13(9), 538.
Fournier, D., 2005. Mutations of acetylcholinesterase which confer insecticide resistance in insect populations. Chemico-Biological Interactions, 158, pp.257–261.
Granada, Y. et al., 2018. A point mutation V419l in the sodium channel gene from natural populations of Aedes aegypti is involved in resistance to λ-cyhalothrin in Colombia. Insects, 9(1), 23.
Hardjanti, A., Donanti, E. & Jakarta, E., 2015. Detection of Insecticide Resistance in Aedes Aegypti to Organophosphate in Pulogadung , East Jakarta Detection of Insecticide Resistance in Aedes aegypti to Organophosphate in. Makara Journal of Health Research, 19(3), pp.117–120.
Harris, A.F., Rajatileka, S. & Ranson, H., 2010. Pyrethroid resistance in Aedes aegypti from Grand Cayman. American Journal of Tropical Medicine and Hygiene, 83(2), pp.277–284.
Haryanto, B. 2018, 'Indonesia Dengue Fever: Status, Vulnerability, and Challenges', in A. J. Rodriguez-Moralez (eds.), Current Topics in Tropical Emerging Diseases and Travel Medicine, IntechOpen.
Hemingway, J. et al., 2004. The molecular basis of insecticide resistance in mosquitoes. Insect Biochemistry and Molecular Biology, 34, pp.653–665.
Imam, H. et al., 2014. The basic rules and methods of mosquito rearing (Aedes aegypti). Tropical Parasitology, 4(1), p.53.
Indonesian Ministry of Health, 1989. Kunci identifikasi Aedes di Jawa. Direktorat Jendral Pemberantasan Penyakit Menular dan Penyehatan Lingkungan.
Indonesian Ministry of Health. 2017. InfoDatin-Situasi-Demam-Berdarah-Dengue. Pusat Data dan Informasi Kementerian Kesehatan RI.
Kawada, H. et al., 2014. Co-occurrence of Point Mutations in the Voltage-Gated Sodium Channel of Pyrethroid-Resistant Aedes aegypti Populations in Myanmar. PLoS Neglected Tropical Diseases, 8(7), e3032.
Kushwah, R.B.S. et al., 2015. Pyrethroid-Resistance and Presence of Two Knockdown Resistance (kdr) Mutations, F1534C and a Novel Mutation T1520I, in Indian Aedes aegypti. PLoS Neglected Tropical Diseases, 9(1), e3332.
Kushwah, R.B.S. et al., 2020. A new knockdown resistance (kdr) mutation, F1534L, in the voltage-gated sodium channel of Aedes aegypti, co-occurring with F1534C, S989P and V1016G. Parasites and Vectors, 13(1), 327.
Lee, H.B. et al., 2016. Allele-specific quantitative PCR for accurate, rapid, and cost-effective genotyping. Human Gene Therapy, 27(6), pp.425–435.
Nathin, M.A., Harun, S.R. & Sumarmo, 1988. Dengue haemorrhagic fever and Japanese B encephalitis in Indonesia. Southeast Asian J Trop Med Public Health, 19(3), pp.475–481.
Rajatileka, S. et al., 2008. Development and application of a simple colorimetric assay reveals widespread distribution of sodium channel mutations in Thai populations of Aedes aegypti. Acta Tropica, 108(1), pp.54–57.
Ranson, H. et al., 2011. Pyrethroid resistance in African anopheline mosquitoes : what are the implications for malaria control? Trends in Parasitology, 27(2), pp.91–98.
Reinert, J.F., Harbach, R.E. & Kitching, I.A.N.J., 2009. Phylogeny and classification of tribe Aedini (Diptera : Culicidae). Zoological Journal of the Linnean Society, 157, pp.700–794.
Russell, T.L. et al., 2011. Increased proportions of outdoor feeding among residual malaria vector populations following increased use of insecticide-treated nets in rural Tanzania. Malaria Journal, 10(1), 80.
Saavedra-Rodriguez, K. et al., 2018. Parallel evolution of vgsc mutations at domains IS6, IIS6 and IIIS6 in pyrethroid resistant Aedes aegypti from Mexico. Scientific Reports, 8(1), 6747.
Setiati, T.E. et al., 2006. Changing epidemiology of dengue haemorrhagic fever in Indonesia. Dengue Bulletin, 30, pp.1–14.
Simmons, C.P. et al., 2012. Dengue. The New England Journal of Medicine, 366, pp.1423–1432.
Srisawat, R. et al., 2010. Point mutations in domain II of the voltage-gated sodium channel gene in deltamethrin-resistant aedes aegypti (diptera: culicidae). Applied Entomology and Zoology, 45(2), pp.275–282.
Stenhouse, S.A. et al., 2013. Detection of the V1016G mutation in the voltage-gated sodium channel gene of Aedes aegypti (Diptera: Culicidae) by allele-specific PCR assay, and its distribution and effect on deltamethrin resistance in Thailand. Parasites and Vectors, 6(1), 253.
World Health Organization, 2006. Dengue. World Health Organization. http://www.searo.who.int/
World Health Organization, 2012. GLOBAL PLAN FOR INSECTICIDE RESISTANCE MANAGEMENT IN MALARIA VECTORS. WHO Library Cataloguing in Publication Data.
World Health Organization, 2020a. Dengue and Severe Dengue. World Health Organization. https://www.who.int/news-room/fact-sheets/detail/dengue-and-severe-dengue
World Health Organization, 2020b. Mosquito borne diseases. World Health Organization. https://www.who.int/neglected_diseases/vector_ecology/mosquito-borne-diseases/en/
World Health Organization, 2020c. Questions and Answers on Dengue Vaccines. World Health Organization. https://www.who.int/immunization/research/development/dengue_q_and_a/en/.
Wuliandari, J.R. et al., 2015. Association between Three Mutations, F1565C, V1023G and S996P, in the Voltage-Sensitive Sodium Channel Gene and Knockdown Resistance in Aedes aegypti from. Insects, 6, pp. 658–685.
Wuliandari, J.R. et al., 2020. Frequency of kdr mutations in the voltage-sensitive sodium channel (V SSC) gene in Aedes aegypti from Yogyakarta and implications for Wolbachia-infected mosquito trials. Parasites and Vectors, 13(1), 429.
Yanola, J. et al., 2011. High-throughput assays for detection of the F1534C mutation in the voltage-gated sodium channel gene in permethrin-resistant Aedes aegypti and the distribution of this mutation throughout Thailand. Tropical Medicine and International Health, 16(4), pp.501–509.
DOI: https://doi.org/10.22146/jtbb.65357
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