Sibling Indices as Comparisons in Personal Identification Process through Short Tandem Repeats [STR] Loci CSF1PO, THOI, TPOX, vWA of Maduranese Ethnic in Surabaya

https://doi.org/10.22146/jtbb.60318

Ahmad Yudianto(1*), Fery Setiawan(2), Simon Martin Manyanza Nzilibili(3)

(1) Department of Forensic Medicine and Medicolegal, Faculty of Medicine, Universitas Airlangga
(2) Forensic Science Master Program, Postgraduate School, Universitas Airlangga
(3) Ministry of Health, Community Development, Gender, Elderly and Children – Tanzania
(*) Corresponding Author

Abstract


Sibling indices can be used as a comparison through alleles Short Tandem Repeats [STR] loci. This is an observational study among Maduranese with 4 STR loci (CSF1PO, THOI, TPOX, vWA) obtained from their blood samples. The percentage of alleles shared: 82.5% [33 times] with 2 allele sharing, 12.5% [5 times] with 1 allele sharing, and 5 % [2 times] with 0 sharing alleles. Sibling indices (SI) calculation results: 65% of sibling indices pairs have SI greater than 100 and 15% of them were between 10-100 (strong and very strong). Sibling indices interpretation is supported; therefore, the claimed sibling indices relationships were indeed true among Maduranese ethnic group in Surabaya.


Keywords


allele sharing; Maduranese; sibling indices; STR DNA

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References

Abbas, S. et al., 2018. Evaluation of sibling indices-ship analysis in secluded Lebanese vilages with increased mating patterns. Journal of Forensic Investigation, 6(1), pp.1-4.

Atmaja, S., A., 2005. Peranan sidik jari DNA pada bidang kedokteran forensic. Materi Workshop DNA fingerprinting.

Butler, J.M., 2006. Genetics and genomics of core STR loci used in human identitytesting. Journal of Forensic Science, 51(2), pp.1–48.

Butler, J.M. & Hill, C.R., 2012. Biology and genetics of new autosomal STR loci useful for forensic DNA Analysis. Forensic Science Review, 24(1), pp.15-26.

Butler J.M., 2015. The future of forensic DNA analysis. Phil Trans R Soc B, 370(1674), pp. 1-10.

Chomczynski, P. et al., 1997. DNAzol: a reagent for therapid isolation of genomic DNA. BioTechniques, 22(3), pp.550–553.

Consentino, E. et al., 2015. Sibling indices incest. Forensic Science International: Genetics, 5, pp.e472-e473.

Fung, W.K. et al., 2004. EasyDNA: user-friendly paternity and kinship testing programs. International Congress Series, 1261, pp.628-630.

Hares, D.R., 2015. Selection and implementation of expanded CODIS core loci inthe United States. Forensic Science International: Genetics, 17, pp.33–34.

Karbeyaz, K. et al., 2016. Case of sibling indices incest resulting in pregnancy. Egyptian Journal of Forensic Sciences, 6(4), pp.550-552.

Karni, M. et al., 2013. Thermal degradation of DNA. DNA and Cell Biology, 32(6), pp.298-301.

Kido, A. et al., 2003. Allele frequency distributions of four STR loci VWA, TH01, TPOX and F13A01 in three Asian populations (Japanese, Bangladeshis and Indonesians). International Congress Series, 1239, pp.105–108.

Maeda, K. et al., 2015. The case of 2 sibling indices that identified not only by DNA profiling. Forensic Science International: Genetics Supplement Series, 5, pp.e555–e556.

Marano, L.A., & Fridman, C., 2019.  DNA phenotyping: current application in forensic sciences. Research and Reports in Forensic Medical Science, 2019(9), pp.1-8, https://www.dovepress.com/by 36.85.41.80 on 11 Sep 2019.

O’Connor, K. L., 2011, Interpretation of DNA Typing Results for Kinship Analysis, USCIS Working Group on DNA Policy, National Institute of Standards and Technology, Washington, DC on 25 January 2011.

Omran, G.A. et al., 2009. Genetic variation of 15 autosomal STR
loci in upper (Southern) Egyptians. Forensic Science International: Genetics,
3(2), pp.e39-e44.

Prastowo, W. et al., 2018. Allele frequencies of STR CODIS 13 of Madura Ethnic from Bangkalan and Probolinggo. Res J Life Sci, 5(2), pp.116–20.

Reid, T.M. et al., 2008. Use of sibling indices pairs todetermine the familial searching efficiency of forensic databases. Forensic Science International: Genetics, 2(4), pp.340–342.

Slooten, K., 2011. Validation of DNA-based identification software by computation of edigree likelihood ratios. Forensic Science International: Genetics, 5(4), pp.308-15.

Sosiawan, A. et al., 2019. Full-Sibling indices Allelic frequency and Sharing among Maduranese: STR Technique by 12 locus and the sex-typing amelogenin gene. Egyptian Journal of Forensic Sciences, 9(38).

Untoro, E. et al., 2009. Allele Frequency of CODIS 13 in Indonesian Population. Legal Medicine, 11(1), pp.S203–S205.

Wenk, R.E. et al., 1996. Determination of sibship in any two persons. Transfusion, 36(3), pp.259–262.

Yamamoto, T. et al., 2003. Allele distributions and genetic relationship with 13 CODIS core STR loci in various Asian populations in or near Japan. International Congress Series, 1239, pp.117-120.

Yudianto, A. & Setiawan, F., 2020. The Effectiveness of Mini Primer STR CODIS in DNA Degradation as the Effect of High-Temperature Exposure. Analytical Cellular Pathology, 2020.



DOI: https://doi.org/10.22146/jtbb.60318

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