Antifungal Activity of Bacterial Isolates from Straw Mushroom Cultivation Medium against Phytopathogenic Fungi

https://doi.org/10.22146/jtbb.59235

Masrukhin Masrukhin(1*), Ade Lia Putri(2), Tri Ratna Sulistiyani(3), Muhammad Ilyas(4), Ismu Purnaningsih(5), Iwan Saskiawan(6), Muhammad Yusrun Niam(7)

(1) Research Center for Biology, Indonesian Institute of Sciences
(2) Research Center for Biology, Indonesian Institute of Sciences
(3) Research Center for Biology, Indonesian Institute of Sciences
(4) Research Center for Biology, Indonesian Institute of Sciences
(5) Research Center for Biology, Indonesian Institute of Sciences
(6) Research Center for Biology, Indonesian Institute of Sciences
(7) Faculty of Science and Technology, UIN Walisongo
(*) Corresponding Author

Abstract


Several bacteria were isolated from straw mushroom (Volvariella volvacea) cultivation medium. There are three potential isolates previously characterized and has growth inhibition effect against V. volvacea. This screening result lead to the further study about the inhibition activity against phytopathogenic fungi. The aim of this research is to investigate the antifungal activity of three bacterial isolates against three phytopathogenic fungi and identification of the bacteria. The method used in this study are antifungal assay using co-culture method and disk difussion assay using the filtrate of each bacteria. The profile of antifungal compound was identified using ethyl acetate extract followed by evaporation and gas chromatography (GC-MS) analysis. Identification of each isolates was performed using 16S rDNA amplification and sequencing. Three phytopathogenic fungi i.e Cercospora lactucae (InaCC F168), Colletotrichum gloeosporides (InaCC F304) and Fusarium oxysporum f.sp. cubense (F817) were co-cultured with bacterial isolates C2.2, C3.8, and D3.3. The C3.8 isolate has highest average inhibition activity either using isolate and filtrate. The result relatively consistent against three phytopathogenic fungi. The metabolite profile of C3.8 isolate showed the Bis(2-ethylhexyl) phthalate as the main compound with 97% similarity. Bis(2-ethylhexyl) phthalate has potential effect as antibacterial and antifungal compound. According to EzBioCloud and GeneBank databases, the C2.2 isolate identified as Bacillus tequilensis, C3.8 as Bacillus siamensis and D3.3 as Bacillus subtilis subsp. Subtilis. This study also shows the potential of Bacillus siamensis C3.8 as biocontrol against phytopathogenic fungi.


Keywords


Antifungal; biocontrol; plant pathogen; bioactive compound; identification

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References

Abdel-Wahab, M.A. et al., 2017. Natural products of Nothophoma multilocularis sp. nov. an endophyte of the medicinal plant Rhazya stricta. Mycosphere, 8(8), pp.1185–1199.

Altschul, S.F. et al., 1997. Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. Nucleic Acid Research, 25(17), pp.3389–3402.

Audinah, L. & Ilmi, M., 2019. Actinomycetes from the soil of chilli plantation in Yogyakarta showing an antagonism to Fusarium oxysporum FU3. Jurnal Biodjati, 4(2), pp.214–224.

Carrasco, J. & Preston, G.M., 2020. Growing edible mushrooms: a conversation between bacteria and fungi. Environmental Microbiology, 22(3), pp.858–872.

Chitarra, G.S. et al., 2003. An antifungal compound produced by Bacillus subtilis YM 10-20 inhibits germination of Penicillium roqueforti conidiospores. Journal of Applied Microbiology, 94(2), pp.159–166.

Chowdhary, K. & Kaushik, N., 2019. Diversity and antifungal activity of fungal endophytes of Asparagus racemosus Willd. Agricultural Research, 8(1), pp.27–35.

Christensen, H. & Olsen, J.E., 2018. Sequence-based classification and identification of prokaryotes. In H. Christensen, ed. Introduction to Bioinformatics in Microbiology. Cham: Springer International Publishing, pp. 121–134.

Cruz-Lagunas, B. et al., 2020. Colletotrichum gloeosporioides causes anthracnose on grapefruit (Citrus paradisi) in Mexico. Australasian Plant Disease Notes, 15(1).

Desmyttere, H. et al., 2019. Antifungal activities of Bacillus subtilis lipopeptides to two Venturia inaequalis strains possessing different tebuconazole sensitivity. Frontiers in Microbiology, 10, pp.1–10.

Dita, M. et al., 2018. Fusarium wilt of banana: Current knowledge on epidemiology and research needs toward sustainable disease management. Frontiers in Plant Science, 871, pp.1–21.

Fira, D. et al., 2018. Biological control of plant pathogens by Bacillus species. Journal of Biotechnology, 285, pp.44–55.

Frey-Klett, P. et al., 2011. Bacterial-fungal interactions: Hyphens between agricultural, clinical, environmental, and food microbiologists. Microbiology and Molecular Biology Reviews, 75(4), pp.583–609.

Habib, M.R. & Karim, M.R., 2009. Antimicrobial and cytotoxic activity of Di-(2-ethylhexyl) phthalate and anhy- drosophoradiol-3-acetate isolated from Calotropis gigantea (Linn.) Flower. Mycobiology, 37(1), pp.31–36.

Jemsi, W.S. & Aryantha, I.N.P., 2017. Potential MGPB in optimizing paddy straw mushroom (Volvariella volvacea WW-08) growth. Microbiology Indonesia, 11(2), pp.46–54.

Jiang, H. et al., 2006. Microbial diversity in water and sediment of Lake Chaka, an athalassohaline lake in northwestern China. Applied and Environmental Microbiology, 72(6), pp.3832–3845.

Kertesz, M.A. & Thai, M., 2018. Compost bacteria and fungi that influence growth and development of Agaricus bisporus and other commercial mushrooms. Applied Microbiology and Biotechnology, 102(4), pp.1639–1650.

Kurian, P.S. et al., 2008. Management of anthracnose disease (Colletotrichum gloeosporioides (Penz) Penz & Sac.) of black pepper (Piper nigrum L.) in the high ranges of Idukki District, Kerala. Journal of Spices and Aromatic Crops, 17(1), pp.21–23.

Li, H. et al., 2018. Isolation and evaluation of endophytic Bacillus tequilensis GYLH001 with potential application for biological control of Magnaporthe oryzae. PLoS ONE, 13(10), pp.1–18.

Li, T. et al., 2020. Co-culture of Trichoderma atroviride SG3403 and Bacillus subtilis 22 improves the production of antifungal secondary metabolites. Biological Control, 140, p.104122.

Lotfy, M.M. et al., 2018. Di-(2-ethylhexyl) Phthalate, a major bioactive metabolite with antimicrobial and cytotoxic activity isolated from River Nile derived fungus Aspergillus awamori. Beni-Suef University Journal of Basic and Applied Sciences, 7(3), pp.263–269.

Lotfy, W.A. et al., 2018. Production of di-(2-ethylhexyl) phthalate by Bacillus subtilis AD35: Isolation, purification, characterization and biological activities. Microbial Pathogenesis, 124, pp.89–100.

Mardanova, A.M. et al., 2017. Bacillus subtilis strains with antifungal activity against the phytopathogenic fungi. Agricultural Sciences, 08(01), pp.1–20.

Masrukhin & Saskiawan, I., 2020. Culturable bacterial abundance in Volvariella volvacea cultivation medium and characterization of its bacteria. Journal of Microbial Systematics and Biotechnology, 2(2), pp.12–21.

McGee, C.F., 2018. Microbial ecology of the Agaricus bisporus mushroom cropping process. Applied Microbiology and Biotechnology, 102(3), pp.1075–1083.

Narayanasamy, P., 2013. Detection and Identification of Fungal Biological Control Agents. In Biological Management of Diseases of Crops. Dordrecht: Springer Netherlands, pp. 9–98.

Nguanhom, J. et al., 2015. Taxonomy and phylogeny of Cercospora spp. from Northern Thailand. Phytotaxa, 233(1), pp.27–48.

Oh, S.Y. & Lim, Y.W., 2018. Effect of fairy ring bacteria on the growth of Tricholoma matsutake in vitro culture. Mycorrhiza, 28(5–6), pp.411–419.

Ortiz, A. & Sansinenea, E., 2018. Di-2-ethylhexylphthalate may be a natural product, rather than a pollutant. Journal of Chemistry, 2018.

Park, K.S. et al., 2012. Evaluation of the GenBank, EzTaxon, and BIBI services for molecular identification of clinical blood culture isolates that were unidentifiable or misidentified by conventional methods. Journal of Clinical Microbiology, 50(5), pp.1792–1795.

Schneider, C.A., Rasband, W.S. & Eliceiri, K.W., 2012. NIH Image to ImageJ: 25 years of image analysis. Nature Methods, 9(7), pp.671–675.

Smaoui, S. et al., 2012. Taxonomy, purification and chemical characterization of four bioactive compounds from new Streptomyces sp. TN256 strain. World Journal of Microbiology and Biotechnology, 28(3), pp.793–804.

Song, B. et al., 2013. Antifungal activity of the lipopeptides produced by Bacillus amyloliquefaciens anti-CA against Candida albicans isolated from clinic. Applied Microbiology and Biotechnology, 97(16), pp.7141–7150.

Than, P.P., Prihastuti, H. & Phoulivong, S., 2008. Chilli anthracnose disease caused by Colletotrichum species. Journal of Zhejiang University Science B, 9(10), pp.764–778.

Usha Nandhini, S. & Masilamani Selvam, M., 2013. GC-MS analysis of antifungal compound produced by Streptomyces from marine soil sediments. Pollution Research, 32(4), pp.787–791.

Vieira, F.R. & Pecchia, J.A., 2018. An exploration into the bacterial community under different pasteurization conditions during substrate preparation (composting–phase II) for Agaricus bisporus cultivation. Microbial Ecology, 75(2), pp.318–330.

Xiang, Q. et al., 2017. The diversity, growth promoting abilities and anti-microbial activities of bacteria isolated from the fruiting body of Agaricus bisporus. Polish Journal of Microbiology, 66(2), pp.201–207.

Yoon, S. et al., 2017. Introducing EzBioCloud : a taxonomically united database of 16S rRNA gene sequences and whole-genome assemblies. International Journal of Systematic and Evolutionary Microbiology, 67, pp.1613–1617.

Zhang, Xiaoyu et al., 2020. Control effects of Bacillus siamensis G-3 volatile compounds on raspberry postharvest diseases caused by Botrytis cinerea and Rhizopus stolonifer. Biological Control, 141(September 2019), p.104135.



DOI: https://doi.org/10.22146/jtbb.59235

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