Callus induction and secondary metabolite profile from  Elephantopus scaber  L.

Junairiah Junairiah; Diah Ayu Wulandari; Edy Setiti Wida Utami; Nabilah Istighfari Zuraidassanaaz

doi:10.22146/jtbb.59234

Callus induction and secondary metabolite profile from Elephantopus scaber L.

https://doi.org/10.22146/jtbb.59234

Junairiah Junairiah^(1*), Diah Ayu Wulandari⁽²⁾, Edy Setiti Wida Utami⁽³⁾, Nabilah Istighfari Zuraidassanaaz⁽⁴⁾

(1) Universitas Airlangga
(2) Universitas Airlangga
(3) Universitas Airlangga
(4) Universitas Airlangga
(*) Corresponding Author

Abstract

Elephantopus scaber L. is a plant that has potential as traditional medicine. Callus induction and production of secondary metabolite content can be increased by culture callus using plant growth regulators. This study was purposed to investigate the effect of IBA and kinetin concentration on the induction and secondary metabolite profile of callus from E. scaber L. leaves. Leaves explant of E. scaber L. were cultured on MS medium with various combination concentrations of IBA and kinetin for 6 weeks and then callus was extracted using methanol. Secondary metabolite content from the resulting extract was analyzed using the phytochemical screening method. The result showed that the treatment of IBA 2.0 mg/L and kinetin 1.0 mg/L and treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L are the fastest combination concentration to induce callus at 5.33 ± 0.577 days. Treatment of IBA 2.0 mg/L and kinetin 2.5 mg/L produced callus with the highest fresh weight and dry weight at 0.7016 ± 0.0588 grams and 0.0766 ± 0.0062 grams, respectively. The morphology of calluses grown during this study was compact with various colors appearance, such as light green, brownish green, and brown. Secondary metabolite content of methanol extract of callus E. scaber L. are flavonoids, alkaloids, terpenoids, and saponins.

Keywords

callus; Elephantopus scaber L.; IBA; kinetin; secondary metabolites

Full Text:

PDF

References

Abraham, J. et al., 2010. A rapid in vitro multiplication system for commercial propagation of pharmaceutically important Cyclea peltata (Lam) Hook & Thoms. based on enhanced axillary branching. Industrial Crops and Products, 31(1), pp.92-98.

Abraham, J. & Thomas, T. D., 2015. Plant regeneration from organogenic callus and assessment of clonal fidelity in Elephantopus scaber Linn., an ethnomedicinal herb. Physiology and Molecular Biology of Plants, 21(2), pp.269-277.

Afshari, R.T. et al., 2011. Effects of light and different plant growth regulators on induction of callus growth in rapeseed (Brassica napus L.) genotypes. Plant Omics Journal, 4(2), pp.60-67.

Arisandi, Y. & Andriani, Y., 2006, Khasiat Berbagai Tanaman untuk Pengobatan, Eska Media, Jakarta.

Asmaliyah, E.E. et al., 2010, Pengenalan Tumbuhan Penghasil Pestisida Nabati dan Pemanfaatannya Secara Tradisional. Kementerian Kehutanan, Badan Penelitian dan Pengembangan Kehutanan, Pusat Penelitian dan Pengembangan Produktivitas Hutan, Palembang.

Bigham, A.K. et al., 2003, Divinatorins A-C, new neoclerodane diterpenoids from the controlled sage Silvia divinorum, Journal of Natural Products, 66(9), pp.1242-1244.

Cheruvathur, M.K. & Thomas, T.D., 2014. High frequency multiple shoot induction from nodal segments and rhinacanthin production in the medicinal shrub Rhinacanthus nasutus (L.) Kurz. Plant Growth Regulation, 74(1), pp.47-54.

Daisy, P. et al., 2007. Hipogligemic and other related effects of Elephantopus scaber extracts on alloxan diabetic rats. Journal of Biological Science, 7(2), pp.433-437.

Darwis, D., 2000, Teknik Dasar Laboratorium dalam Penelitian Senyawa Bahan Alam Hayati, Workshop Pengembangan Sumber Daya Manusia Dalam Bidang Kimia Organik Bahan Alam Hayati, Universitas Andalas, Padang.

George, F.P. & Sherrington, P.D., 2007, Plant Propagation by Tissue Culture, Eversley, Hand Book and Directory of Commercial Laboratories Exigetic Limited.

Grayson, D.H., 2000, ‘Monoterpenoid’, Natural Product Report. University Chemical Laboratory, Trinity College, Ireland.

Habibah, N.A. et al., 2018. Callus induction and flavonoid production on the immature seed of Stelechocarpus burahol. In Journal of Physics: Conference Series 983, 012186.

Harborne, J.B., 2006, Metode Fitokimia Penuntun Cara Modern Menganalisis Tumbuhan, ITB, Bandung.

Ho, W.Y. et al., 2012. Hepatoprotective activity of Elephantopus scaber on alcohol-induced liver damage in mice. Evidence-Based Complementary and Alternative Medicine, 2012, 417953.

Jain, S.C. et al., 2012. In-vitro callus propagation and secondary metabolite quantification in Sericostoma pauciflorum. Iranian Journal of Pharmaceutical Research: IJPR, 11(4), pp.1103-1109.

Jamwal, K. et al., 2018. Plant growth regulator mediated consequences of secondary metabolites in medicinal plants. Journal of Applied Research on Medicinal and Aromatic Plants, 9(2018), pp.26-38.

Kristanti, A.N. et al., 2008, Buku Ajar Fitokimia, Airlangga University Press, Surabaya.

Kumar, G.K. & Thomas, T.D., 2012. High frequency somatic embryogenesis and synthetic seed production in Clitoria ternatea Linn. Plant Cell, Tissue and Organ Culture, 110(1), pp.141-151.

Lim, S.Y. et al. 2006. Phytol-based novel adjuvants in vaccine formulation: 2. assessment of efficacy in the induction of protective immune responses to lethal bacterial infections in mice. Journal of Immune Based Therapies and Vaccines, 4(1), pp.5.

Manuhara, Y.S.W., 2014, Kapita Selekta Kultur Jaringan Tumbuhan, Airlangga University Press, Surabaya.

Murthy, H.N. et al., 2014. Production of secondary metabolites from cell and organ cultures: strategies and approaches for biomass improvement and metabolite accumulation. Plant Cell, Tissue and Organ Culture, 118(1), pp.1-16.

Nasri, E., 2012, Pengaruh pemberian ekstrak etanol terhadap kadar LDL (Low Density Lipoprotein) pada mencit putih jantan, Skripsi, Sekolah Tinggi Ilmu Farmasi, Padang.

Nurtamin, T. et al., 2018. In vitro anti-inflammatory activities of ethanolic extract Elephantopus scaber leaves. Indonesian Journal of Medicine and Health, 9(1), pp.46-52.

Parashurama, T.R. et al. 2013. Colletotrichum disease of Elephantopus scaber and its effect on secondary metabolites. International Journal of Pharmaceutical and Bioscience, 4, pp.871-883.

Payghamzadeh, K. & Kazemitabar, S.K., 2011. In vitro propagation of walnut – a review. African Journal of Biotechnology, 10(3), pp.290-311.

Puspita, 2004, Efek diuretik pada pemberian infusa daun tapak liman (Elephantopus scaber L.) pada tikus jantan galur wistar, Skripsi. Universitas Muhammadiyah, Surakarta.

Radić, S. et al., 2016. Influence of pH and plant growth regulators on secondary metabolite production and antioxidant activity of Stevia rebaudiana (Bert). Periodicum Biologorum, 118(1), pp.9-19.

Rohmatin, N. 2014. Induksi kalus dari eksplan daun gandarusa (Justicia gendarusa Burm.f.) dengan pemberian kombinasi zat pengatur tumbuh 2,4-D, IBA dan Kinetin. Skripsi. Universitas Airlangga. Surabaya.

Rosyidah, M. et al., 2014. Induksi kalus daun melati (Jasminum sambac) dengan penambahan berbagai konsentrasi dichlorophenoxyacetic acid (2,4-D) dan 6-benzylamino purin (BAP) pada media MS secara in vitro, Jurnal Biologi, 3(3), pp.147-153.

Rout, J.R. & Sahoo, S.L., 2013. In vitro propagation and antioxidant enzymes activities of Elephantopus scaber L. Asia-Pacific Journal of Molecular Biology and Biotechnology, 21, pp.51-66.

Rumondang, M. et al., 2013. Isolasi, identifikasi, dan uji antibakteri senyawa triterpenoid dari ekstrak n-heksana daun tempuyung (Sochus arvensis L.), Chem Info Journal, 1, pp.156-164.

Sankar, V. et al., 2001. Antiinflammatory activity of Elephantopus scaber in albino rats. Indian Journal of Pharmaceutical Sciences, 63(6), pp.523-525.

Siddique, A.B. & Islam, S.S., 2015. Effect of light and dark on callus induction and regeneration in tobacco (Nicotiana tabacum L.). Bangladesh Journal of Botany, 44(4), pp.643-651.

Sulastri, 2008, Efek diuretik ekstrak etanol 70% daun tapak liman (Elephantopus scaber L.) pada tikus jantan galur wistar, Skripsi, Universitas Muhammadiyah, Surakarta.

Wang, J. et al., 2014. Bioactivities of compounds from Elephantopus scaber, an ethnomedicinal plant from southwest China. Evidence-Based Complementary and Alternative Medicine, 2014, 569594.

Yang, X. & Chen, G., 2014. Stimulation of photosynthetic characteristics of Ginkgo biloba L. during leaf growth. Bangladesh Journal of Botany, 43(1), pp.73-77.

Yuniarti, T., 2008, Ensiklopedia Tanaman Obat Tradisional, Jakarta.

DOI: https://doi.org/10.22146/jtbb.59234