Isolation and Identification of Endophytic Fungi from Leave and Stem of Calopogonium mucunoides

https://doi.org/10.22146/jtbb.32477

Dian Fitriarni(1*), Rina Sri Kasiamdari(2)

(1) Agricultural Technology, Politeknik Negeri Ketapang
(2) Faculty of Biology, Universitas Gadjah Mada
(*) Corresponding Author

Abstract


Thirty-seven isolates of endophytic fungi were isolated from leaves and stems of Calopogonium mucunoides collected from PTPN PTPN XII (Persero) Rubber Plantation, Klatakan, Kecamatan Tanggul, Kabupaten Jember, Jawa Timur. All isolates were identified based on morphological characteristics using the light microscope. The 37 isolates of endophytic fungi are members of Deuteromycota and Basidiomycota and classified to genera Phoma, Phomopsis, Corynespora, Rhizoctonia, Helicosporium, Curvularia, Torulomyces, Gliocladium, Gloeosporium, Acremonium, Tripospermum, Aureobasidium, Colletotrichum, Humicola, Fusarium, Sclerotium, and sterile hyphae.

 

 


Keywords


endophytic fungi; Calopoginium mucunoides; isolation; identification

Full Text:

PDF


References

Alexopoulus, J., C. Mims, and M. Blackwell. 1996. Introductory Mycology. John Wiley & Sons. Inc. New York

Anita, D. D and Sridhar, KR. 2009. Assemblage and Diversity of Fungi Associated With Mangrove Wild Legume Canavalia cathartica.  Tropical and Subtropical Agroecosystems. 10:225 – 235.

Barnett, H.L and Hunter.1972. Ilustrated Genera of Imperfect Fungi. Burger Publishing Minneapolis Minesota. 3rd edition. P: 116-117.

Binggeli, P. 1996. A taxonomic, biogeographical and ecological overview of invasive woody plants. J. Veg. Sci. 7: 121-124.s

Buatong, J. 2010. Endophytic Fungi Producing Antimicrobial Substances from Mangrove Plants in South Thailand. Prince of Songkla University. Thailand.

Dandu, A., Reddy, N. V., Venkateswarlu, N., Pushpalatha, B., and Vijaya, T. 2014. Molecular identification of endophytic fungi isolated from Rhynchosia beddomei, an endemic medicinal plant of Tirumala Hills.  Res. J. Pharm, Biol. Chem. Sci. 5:1280-1285.

Dandu, A., Reddy, N. V., Venkateswarlu, N., Pushpalatha, B., and Vijaya, T. 2014. Molecular identification of endophytic fungi isolated from Rhynchosia beddomei, an endemic medicinal plant of Tirumala Hills.  Res. J. Pharm, Biol. Chem. Sci. 5:1280-1285.

Enechi,  O.C., Odo’, C.E.,  and Okafor, C. 2014. Assessment of the anti-ulcer action of the leaves of Calopo (Calopogonium mucunoides Desv) in Wistar rats.  J. Pharm. Res.8(1):24-27.

Fernandes et. Al. 2011. Endophyte diversity mediates leaf optical properties. http://www.isprs.org/proceedings/2011/ISRSE-34/211104015Final00609.pdf. 17 Desember 2015.

Faeth, S.H., and Fagan, W.F. 2002. Fungal Endophytes: Common Host Plant Symbiont but Uncommon Mutualism. Integ. and Comp. Biot. 43:360-368.

Gandjar, I., R.A. Samson, K. van den Tweel-Vermeulen, Oetari, A dan Santoso, I.  1999. Pengenalan kapang tropik umum, Yayasan Obor Indonesia, Jakarta.

Gandjar, I., dan R.A. Sjamsuridzal, W.,  dan Oetari, A. 2006. Mikologi dasar dan terapan, Yayasan Obor Indonesia, Jakarta.

Hidayati N., Fauzia S, Titi J. 2005. Potensi Centrocema pubescence, Calopogonium mucunoides, dan Micania cordota dalam membersihkan logam kontaminan pada limbah penambangan emas. Pusat Penelitian Biologi, Lembaga Ilmu Pengetahuan Indonesia (LIPI). Bogor.

Idu, M., and Onyibe, H.I. 2007. Medicinal plant of Edo State, Nigeria. J. Med. Plant. 1(2):32-41

Katoch M., Singh, G., Sharma, S., Gupta, N., Sangwan, P.L., and Saxena, A. K. 2014. Cytotoxic and antimicrobial activities of endophytic fungi isolated from Bacopa monnieri (L.) Pennell (Scrophulariaceae). BMC Complementary and Alternative Medicine. 14:1-8.

Marcia González-Teuber, Guillermo H Jiménez-Alemán, & Wilhelm Boland. 2014. Foliar endophytic fungi as potential protectors from pathogens in myrmecophytic Acacia plants. Communicative & Integrative Biol. 7(5):1-4.

Mousa, W.K., and Raizada, M.N. 2013. Review: The diversity of anti-microbial secondary metabolites produced by fungal endophytes: an interdisciplinary perspective. FCIMB. 4(65):1-18.

Petrini, O. 1991. Fungal endophytes of tree leaves. In: Andrews JH and Hirano SS, (Eds). Microbial Ecology of Leaves. Spring Verlag. New York. 179-197.

Phowtong, P., Jantrapanukorn, B., Thongmee, A., and Suntornthiticharoen, P. 2013. Screening of antimicrobial activities of the endophytic fungi isolated from Sesbania grandiflora (L.) Pers. J. Agr. Sci. Technol. 15: 1513-1522.

Pimentel, I. C., Glienke-Blanco, C., Gabardo, J., Stuart, R. M., and Azevedo, J. L. 2006. Identification and Colonization of Endophytic Fungi from Soybean (Glycine max (L.) Merril) under Different Environmental Conditions. Brazilian Archives Of Biol. Technol. An Intern. J. 49(5): 705-711.

Pitt, J.I. and A.D. Hocking, 2009. Fungi and Food Spoilage. 3rd Edn. Springer, USA. 519.

Rodriguez, R. J., Arnold, J. F., White Jr, A. E. and Redman, R. S. 2008. Review Fungal endophytes: diversity and functional roles. New Phytol. 1-17.

Ramesha, A., and Srinivas, C. 2014. Antimicrobial activity and phytochemical analysis of crude extracts of endophytic fungi isolated from Plumeria acuminata L. and Plumeria obtusifolia L. Europe J. Exp. Biol. 4(2):35-43.

Roza, L.V., Chanda, A., & Linz, J.E. 2011. Compartmentalization and molecular traffic in secondary metabolism: a new understanding of established cellular processes. Fungal Genet. Biol. 48: 35–48.

Santos, IP, Silva, LCN, Silva, MV, Araújo, JM, Cavalcanti, MS and Lima, VLM (2015) Antibacterial activity of endophytic fungi from leaves of Indigofera suffruticosa Miller (Fabaceae). Front. Microbiol. 6:350.

Sedlář J., Sedlářová M., and Flusser J. 2009. Image Processing Methods For Determination Of Downy Mildews From Light Microscopy Images. In: Kulpa K, Kaska W.(Eds) Signal Processing Symposium Proceedings, Warsaw University of Technology, Warsaw.

Selim, KA, El-Beih, A.A, AbdEl-Rahman, TM, and El-Diwany, AI. 2012. Biology of endophytic fungi. Curr. Res. Environ. Appl. Mycol. 2(1):31–82.

Senna, S., and Sridhar, K.R. 2004. Endophytic fungal diversity of 2 sand dune wild legumes from the southwest coast of India. Canadian J. Microbiol. 50: 1015–1021.

Schulz, B., Drager, U.W.S., and Aust, HJ. 1993. Endophytes from herbaceous plants and shrubs: effectiveness of surface sterilization methods. Mycol. Res. 97: 1447-1450.

Schulz, B., and Boyle, C. 2005. The endophyte continumm. Mycol. Res. 109:661-686.

Shankar, N.B., and Shashikala, J. 2010. Diversity and structure of fungal endophytes in some climbers and grass species of Malnad Region, Western Ghats, Southern India. Mycosphere. 1:265-274.

Shenoy, B.D., Jeewon, R., and Hyde, K.D. 2007. Impact of DNA sequence-data on the taxonomy of anamorphic fungi. Fungal Divers.26: 1-54.

Sihombing A., Fatonah, S., and Silviana F. 2012. Pengaruh alelopati Calopogonium mucunoides Desv. terhadap perkecambahan dan pertumbuhan anakan gulma Asystasia gangetica (L.) T. Anderson. J. Biospecies. 5 (2): 5 – 11.

Stone, J.K., Polishook J.D., and White J.F. 2004. Endophytic Fungi. In: Biodiversity of Fungi. Inventory and Monitoring Methods. In Mueller G.M., Bills G.F., Foster M.S.(Eds) Elsevier Academic Press, San Diego, United State of America. 241-270.

Taylor, J.W. 2011. One Fungus, One Name: DNA and fungal nomenclature twenty years after PCR. IMA Fungus. 2(2): 113–120.

Venkatesan G, Suryanarayanan S. 2013. Fungi associated with the leaves of some hydrophyte plants. Int. J. Curr. Res. Dev.1 (1): 53-69.

White, R.E. 2009. Principles and practices of soil science.Wiley-Blackwell. 384

Zuber, A., Kowalczyk, M., Sekula, A., Mleczko, P., and Kupiec, T. 2011. Method used in species identification of hallucinogenic and other poisonuous mushrooms in forensic investigations. Prob. Foren. Sci. 86:151–161.

 



DOI: https://doi.org/10.22146/jtbb.32477

Article Metrics

Abstract views : 531 | views : 407

Refbacks

  • There are currently no refbacks.


Copyright (c) 2018 Journal of Tropical Biodiversity and Biotechnology

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Editoral address:

Faculty of Biology, UGM

Jl. Teknika Selatan, Sekip Utara, Yogyakarta, 55281, Indonesia

ISSN: 2540-9573 (print), 2540-9581 (online)