Bioaktivitas ekstrak batang Xylocarpus granatum sebagai anti black spot alternatif pada Litopenaeus vannamei pasca panen
Lanny Kartikasari(1), Awik Pudji Diah Nurhayati(2*), Edwin Setiawan(3), Dewi Hidayati(4), Nova Maulidina Ashuri(5), Noor Nailis Saadah(6), Farid Kamal Muzaki(7), Iska Desmawati(8)
(1) Program Studi Biologi, Institut Teknologi Sepuluh Nopember, Surabaya
(2) Program Studi Biologi, Institut Teknologi Sepuluh Nopember, Surabaya
(3) Program Studi Biologi, Institut Teknologi Sepuluh Nopember, Surabaya
(4) Program Studi Biologi, Institut Teknologi Sepuluh Nopember, Surabaya
(5) Program Studi Biologi, Institut Teknologi Sepuluh Nopember, Surabaya
(6) Program Studi Biologi, Institut Teknologi Sepuluh Nopember, Surabaya
(7) Program Studi Biologi, Institut Teknologi Sepuluh Nopember, Surabaya
(8) Program Studi Biologi, Institut Teknologi Sepuluh Nopember, Surabaya
(*) Corresponding Author
Abstract
This research was done to explore bioactivity of Xylocarpus granatum’s stem extract as tyrosinase inhibitor for alternative preventing black spot in Litopenaeus vannamei postharvest. In this research, extraction of bioactive compound from X. granatum stem was done by soaking the stem in 96% methanol for 24h at temperature 29°C. The methanol was evaporated by using rotary evaporator at temperature 50°C. The extract was dilute with distillate water at series of dilutions (10%, 30%, 50%, 70%, 90%, and distillate water as the control), and then immersing L. vannamei into X. granatum’s stem extract as much as 10ml for 6h, and finally the data analyzing use the Kruskal Wallis test and the Least Significant Difference (LSD) test with the degree of confident 95%. The result showed that X. granatum’s stem extract is able to prevent a black spot of L. vannamei after harvesting process. Furthermore, concentration of 10 % of extract is an effective concentration for preventing a black spot and the control has black spot 0.0360 – 0.0373 mm2.
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Badan Pengawas Obat dan Makanan Republik Indonesia, 2012, Sodium Sulfit, Sentra Informasi Keracunan Nasional (SIKerNas), Pusat informasi obat dan makanan, http://jdih.pom.go.id ,viewed 19 April 2017
Badan Standarisasi Nasional,1998, SNI 0648241-1998: Persyaratan residu chlorine pada air minum, Jakarta, http://sisni.bsn.go.id, viewed 19 April 2017
Benjakul, S., Visessanguan,W., and Tanaka, M., 2005, Properties of phenoloxidase isolated from the cephalothorax of Penaeus japonicas, Journal of Food Biochemistry 29, 470-485.
Chang, T., 2009, An updated review of tyrosinase inhibitors, Int. J. Mol. Sci 10, 2440-2475.
Gimenez, B., 2004, Characteriztion of phenoloxidase activity of carapace and viscera from cephalothorax of norway lobster (Nephrops norvegicus), CSIC: Madrid.
Graillet, C.R., Edith, A., Monique,C., Ortonne, J. P., and Robert, B., 1997, Nitric oxide produced by ultraviolet-irradiated keratinocytes stimulates melanogenesis, J Clin Invest 99(4),635-642.
Hartanti, L., & Setiawan, H. K., 2009, Inhibitory potential of some synthetic cinnamic acid derivatives towards tyrosinase, Indo J Chem 9, 158-168.
Hendrawan, Zuraida, I., dan Pamungkas, B. F., 2015, Aktivitas antibakteri ekstrak metanol X. granatum dari pesisir muara badak, Jurnal Ilmu Perikanan Tropis 20(2), 15-22.
Hunter, J., Savin, J., and Dahl, M., 2003, Clinical dermatology 3rd edition, Blackwell Science Ltd: Malden.
Likhitwitayawuid, K., 2008, Stilbenes with tyrosinase inhibitory activity, Review Article Current Science 94(1), 44-52.
Lopolisa, C., Darusman, L. K., and Batubara, I., 2011, Screening marker component of tyrosinase inhibitor from X. granatum stem, Valensi 2(3), 409-413.
Manheem, K., Benjakul, S., Kijroongrojana, K., Faithong, N., and Visessangar, 2013, Effect of precooking times on enzymes, properties, and melanosis of pacific white shrimp during refrigerated storage, International Aquatic Research, Springer 5(1), 1-11.
Miget, R., 2010, Shellfish handling practices–shrimp and molluscs, SRAC Publication No. 4902, Texas.
Min, K. R., Kim, K. S., Ro, J. S., Lee, S. H., Kim, J. A., Son, J. K., and Kim, Y., 2004, Piperlonguminine from Piper longum with inhibitory effects on alphamelanocyte-stimulating hormone-induced melanogenesis in melanoma B16 cells. Planta Med 70, 1115-1118.
Shosuke, I., 2003, A chemist view of melanogenesis, Pigment Cell Res 16, 230–236.
DOI: https://doi.org/10.22146/jtbb.16385
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