IIsolasi dan Identifikasi Staphylococcus aureus pada Susu Sapi Mastitis Subklinis di Pangalengan, Jawa Barat

Hemalya Sylvany; Pranyata Tangguh Waskita; Sarasati Windria

doi:10.22146/jsv.87051

IIsolasi dan Identifikasi Staphylococcus aureus pada Susu Sapi Mastitis Subklinis di Pangalengan, Jawa Barat

https://doi.org/10.22146/jsv.87051

Hemalya Sylvany⁽¹⁾, Pranyata Tangguh Waskita^(2*), Sarasati Windria⁽³⁾

(1) Padjajaran University
(2) Program Studi Kedokteran Hewan, Fakultas Kedokteran, Universitas Padjajaran, Jawa Barat
(3) 2Departemen Ilmu Kedokteran Dasar, Divisi Mikrobiologi, Fakultas Kedokteran, Universitas Padjadjaran, Jawa Barat
(*) Corresponding Author

Abstract

Susu sapi merupakan produk utama yang dihasilkan sapi perah. Kandungan susu sapi yang beragam, menjadikan susu sebagai sumber nutrisi yang baik untuk metabolisme tubuh. Tingginya permintaan susu di Indonesia belum terpenuhi karena adanya aspek kesehatan dari sapi yang mengalami mastitis. Staphylococcus aureus adalah salah satu patogen yang menyebabkan mastitis subklinis pada sapi. Tujuan dari penelitian ini adalah untuk mengetahui cemaran Staphylococcus aureus pada sampel susu sapi yang menderita mastitis subklinis di Pangalengan. Penelitian ini dilakukan dengan metode deskriptip kualitatif, menggunakan 48 sampel susu sapi perah mastitis dengan hasil uji CMT . Isolasi dan identifikasi Staphylococcus aureus dilakukan dilakukan melalui metode kultur pada media Blood Agar Plate (BAP), Mannitol Salt Agar (MSA), pewarnaan Gram, uji katalase, uji DNase, serta uji koagulase. Dari 48 sampel terdapat 17 isolat yang mengandung Staphylococcus aureus. 17 isolat menghasilkan hemolisin pada blood agar, memfermentasikan mannitol, pewarnaan Gram positif kokus, katalase positif, koagulase positif dan DNase positif.

Kata kunci : mastitis subklinis; susu sapi; Staphylococcus aureus.

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References

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DOI: https://doi.org/10.22146/jsv.87051