* Corresponding Author
ISOLATION, IDENTIFICATION AND ANALYSIS OF SHORT INTERSPERSED NUCLEAR ELEMENT (SINE) FROM VARANUS KOMODOENSIS
https://doi.org/10.22146/jsv.8591 Widya Asmara(1*)
(1) Bagian Mikrobiologi Fakultas Kedokteran Hewan UGM Yogyakarta
(*) Corresponding Author
Abstract
All vertebrata genomes studied so far contain SINEs, that consti tute up to 5% of the total genome. Most of the SINE sequences studied so far are mammalian genomes. There Is very limited information about SINE sequences from reptiles, especially of Varanus family.
Isolation of SINE sequences were carried out by constructing the komodo genomic library in E. coli. Suspected positive clones were detected by genome to genome hybridization. The SINE sequences identification were carried out by enzymatic method, and the DNA sequences were analysed with the GENETYX-SV program-Up to now there are 2 SINE sequences from komodo genome have been isolated and identified. Their sequences homo logy is 85-2% From Database search analysis with the GenBank indicated that those SlNEs were not evolved from any specific tRNA gene.
Keywords
DOI: https://doi.org/10.22146/jsv.8591
Article Metrics
Abstract views : 1241
Refbacks
- There are currently no refbacks.
Copyright (c) 2015 Jurnal Sain Veteriner
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.
Jurnal Sain Veteriner Indexed by
Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).
Fakultas Kedokteran Hewan, Universitas Gadjah Mada
Jl. Fauna No.2, Karangmalang, Yogyakarta
Phone: 0274-560862
Fax: 0274-560861
Email: jsv_fkh@ugm.ac.id
HP. 0895363078367
Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.