Skrining Susu terhadap Mycobacterium bovis pada Peternakan Sapi Perah di Wilayah Tengah dan Timur Pulau Jawa, Indonesia

https://doi.org/10.22146/jsv.82787

Andi Eka Putra(1), Chaerul Basri(2*), Etih Sudarnika(3), Santi Lestari(4)

(1) Direktorat Kesehatan Masyarakat Veteriner Kementerian Pertanian Republik Indonesia Jl. Harsono RM Jakarta
(2) Sekolah Kedokteran Hewan dan Biomedis Institut Pertanian Bogor
(3) Sekolah Kedokteran Hewan dan Biomedis Institut Pertanian Bogor
(4) Balai Besar Veteriner Wates Yogyakarta
(*) Corresponding Author

Abstract


Tuberkulosis adalah penyakit dengan tingkat kejadian kasus tinggi pada masyarakat di Indonesia. Bovine tuberculosis pada sapi perah disebabkan oleh bakteri M. bovis, diduga turut berperan dalam menyebabkan kasus tuberkulosis karena dapat menular ke manusia (zoonosis) melalui konsumsi susu. Wilayah tengah dan timur Pulau Jawa merupakan sentra peternakan sapi perah di Indonesia yang masih melaporkan tingginya kasus tuberkulosis pada manusia. Informasi mengenai keberadaan bakteri M. bovis pada susu yang dihasilkan di wilayah tersebut belum pernah dilaporkan. Penelitian ini bertujuan untuk skrining keberadaan M. bovis di susu pada peternakan sapi perah di wilayah tengah dan timur Pulau Jawa. Sampel susu diambil dari 163 ekor sapi menggunakan metode cluster random sampling dari 3 provinsi yaitu Jawa Tengah, Yogyakarta dan Jawa Timur. Sampel susu diuji dengan menggunakan PCR konvensional dengan metode multiplex PCR untuk mengidentifikasi M. bovis dan M. tuberculosis dalam waktu bersamaan. Skrining dengan uji PCR terhadap 163 sampel susu dari peternakan sapi perah di wilayah tengah dan timur Pulau Jawa menunjukkan bahwa susu yang dihasilkan oleh sapi perah yang dipelihara di wilayah ini tidak mengandung bakteri M. Bovis dan M. Tuberculosis.


Keywords


bovine tuberculosis; M. bovis; PCR; susu; zoonosis

Full Text:

PDF


References

Al-Thwani, A.N., Al-Saqur, I.M., and Al-Attar, I.M. (2015). Comparative study between routine and molecular diagnosis of Mycobacterium species isolated from humans and bovines. International Journal of Mycobacteriology. 4: 141–142. Arenas, N.E., Abril, D.A., Valencia, P., Khandige, S., Soto, C.Y., and Moreno-Melo, V. (2017). Screening food-borne and zoonotic pathogens associated with livestock practices in the Sumapaz region, Cundinamarca, Colombia. Tropical Animal Health and Production. 49 (4): 739–745. Badan Pusat Statistik. (2022). Produksi susu segar menurut provinsi 2020-2022. Retrieved March 02, 2023, from https://www.bps.go.id/indicator/24/493/1/produksi-susu-segar-menurut-provinsi.html. Basit, A., Hussain, M., Shahid, M., Ayaz, S., Rahim, K., Ahmad, I., Rehman, A.U., Hassan, M.F., and Ali, T. (2018). Occurrence and risk factors associated with Mycobacterium tuberculosis and Mycobacterium bovis in milk samples from North East of Pakistan. Pakistan Veterinary Journal. 38 (2): 199–203. Bhanu Rekha, V., Gunaseelan, L., Pawar, G., Nassiri, R., and Bharathy, S. (2015). Molecular detection of Mycobacterium tuberculosis from bovine milk samples. Journal of Advanced Veterinary and Animal Research. 2 (1): 80–83. Bezerra, A.V.A., Dos Reis, E.M., Rodrigues, R.O., Cenci, A., Cerva, C., and Mayer, F.Q. (2015). Detection of Mycobacterium tuberculosis and Mycobacterium avium complexes by real-time PCR in bovine milk from Brazilian dairy farms. Journal of Food Protection. 78 (5): 1037–1042. Center for Disease Control and Prevention. (2011). Bovine TB in Human Fact Sheet. Retrieved March 02, 2023, from https://www.cdc.gov/tb/publications/factsheets/general/mbovis.htm Cezar, R.D.S., Lucena-Silva, N., Borges, J.M., Santana V.L.A., and Pnheiro Junior, J.W. (2016). Detection of Mycobacterium bovis in artisanal cheese in the state of Pernambuco, Brazil. International Journal of Mycobacteriology. 5 (3): 269–272. Christi, F.R., Salman, L.B., and Sudrajat, A. (2022). Manajemen penerapan konsep biosecurity di peternakan sapi perah Kecamatan Sukalarang kabupaten sukabumi jawa barat. Farmers: Journal of Community Services. 03 (2): 19–23. Collins, B., Floyd, S., Gordon, S.V., and More, S.J. (2022). Prevalence of Mycobacterium bovis in milk on dairy cattle farms: An international systematic literature review and meta-analysis. Tuberculosis. 132: 102166. Daulay, M.U. (2015). Kajian Prevalensi Dan Faktor Risiko Penyakit Tuberkulosis Pada Sapi Perah di Wilayah Bogor Serta Pengembangan Media Kultur Mycobacterium Bovis. Dissertation. Fakultas Kedokteran Hewan Institut Pertanian Bogor, Bogor. Ereqat, S., Nasereddin, A., Levine, H., Azmi, K., Al-Jawabreh, A., Greenblatt, C.L., Abdeen, Z., and Bar-Gal, G.K. (2013). First-time detection of Mycobacterium bovis in livestock tissues and milk in the West Bank, Palestinian Territories. PLoS Neglected Tropical Diseases. 7 (9). Franco, M.M.J., Paes, A.C., Ribeiro, M.G., de Figueiredo Pantoja, J.C.A., Santos C.B., Miyata, M., Leite, C.Q.F., Motta, R.G., and Listoni, F.J.P. (2013). Occurrence of mycobacteria in bovine milk samples from both individual and collective bulk tanks at farms and informal markets in the southeast region of Sao Paulo, Brazil. BMC Veterinary Research. 9. Jayasumana, M.T.L.K., Galappaththi, T.I., Pushpakumara, P.G.A., Gamage, C.D., Smith, N.H., and Jinadasa, H.R.N. (2018). Screening milk for bovine tuberculosis in dairy farms in Central Province, Sri Lanka. Trop Agric Res. 30: 12–8. Kock, R., Michel, A. L., Yeboah-Manu, D., Azhar, E. I., Torrelles, J. B., Cadmus, S. I., Brunton, L., Chakaya, J. M., Marais, B., Mboera, L., Rahim, Z., Haider, N., and Zumla, A. (2021). Zoonotic tuberculosis – the changing landscape. International Journal of Infectious Diseases. 113: S68–S72. Lan, Z., Bastos, M., and Menzies, D. (2016). Treatment of human disease due to Mycobacterium bovis: A systematic review. European Respiratory Journal. 48(5): 1500–1503. Michel, A.L., Müller, B., and van Helden, P.D. (2010). Mycobacterium bovis at the animal–human interface: A problem, or not. Vet Microbiol. 140:371–381. Nahar, Q., Pervin, M., Islam, M., and Khan, M. (2011). Application of PCR for the detection of bovine tuberculosis in cattle. J. Bangladesh Agril. Univ. 9: 73-78. Office International des Epizooties. (2020). Bovine Tuberculosis. OIE Terrestrial Manual. Retrieved January 22, 2023, from https://rr-asia.oie.int/en/projects/bovine-tuberculosis/. Simarmata, Y. T. (2014). Identifikasi isolat Mycobacterium bovis dengan konsentrasi DNA bertingkat menggunakan Teknik Polymerase Chain Reaction. Jurnal Kajian Veteriner. 2(2): 167–173. World Health Organization. (2020). World Health Organisation. Global tuberculosis report 2020. Retrieved January 28, 2023, from https://www.who.int/publications/i/item/9789240013131.



DOI: https://doi.org/10.22146/jsv.82787

Article Metrics

Abstract views : 543 | views : 586

Refbacks

  • There are currently no refbacks.




Copyright (c) 2023 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats