Skrining Susu terhadap Mycobacterium bovis pada Peternakan Sapi Perah di Wilayah Tengah dan Timur Pulau Jawa, Indonesia

https://doi.org/10.22146/jsv.82787

Andi Eka Putra(1), Chaerul Basri(2*), Etih Sudarnika(3), Santi Lestari(4)

(1) Direktorat Kesehatan Masyarakat Veteriner Kementerian Pertanian Republik Indonesia Jl. Harsono RM Jakarta
(2) Sekolah Kedokteran Hewan dan Biomedis Institut Pertanian Bogor
(3) Sekolah Kedokteran Hewan dan Biomedis Institut Pertanian Bogor
(4) Balai Besar Veteriner Wates Yogyakarta
(*) Corresponding Author

Abstract


Tuberkulosis adalah penyakit dengan tingkat kejadian kasus tinggi pada masyarakat di Indonesia. Bovine tuberculosis pada sapi perah disebabkan oleh bakteri M. bovis, diduga turut berperan dalam menyebabkan kasus tuberkulosis karena dapat menular ke manusia (zoonosis) melalui konsumsi susu. Wilayah tengah dan timur Pulau Jawa merupakan sentra peternakan sapi perah di Indonesia yang masih melaporkan tingginya kasus tuberkulosis pada manusia. Informasi mengenai keberadaan bakteri M. bovis pada susu yang dihasilkan di wilayah tersebut belum pernah dilaporkan. Penelitian ini bertujuan untuk skrining keberadaan M. bovis di susu pada peternakan sapi perah di wilayah tengah dan timur Pulau Jawa. Sampel susu diambil dari 163 ekor sapi menggunakan metode cluster random sampling dari 3 provinsi yaitu Jawa Tengah, Yogyakarta dan Jawa Timur. Sampel susu diuji dengan menggunakan PCR konvensional dengan metode multiplex PCR untuk mengidentifikasi M. bovis dan M. tuberculosis dalam waktu bersamaan. Skrining dengan uji PCR terhadap 163 sampel susu dari peternakan sapi perah di wilayah tengah dan timur Pulau Jawa menunjukkan bahwa susu yang dihasilkan oleh sapi perah yang dipelihara di wilayah ini tidak mengandung bakteri M. Bovis dan M. Tuberculosis.


Keywords


bovine tuberculosis; M. bovis; PCR; susu; zoonosis

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References

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DOI: https://doi.org/10.22146/jsv.82787

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