Identifikasi Fenotip dan Genotip Staphylococcus aureus Isolat Asal Susu Sapi Perah Mastitis Subklinis di Wilayah Pamulihan, Kabupaten Sumedang Jawa Barat

https://doi.org/10.22146/jsv.76052

Sarasati Windria(1*), Alifya Azzahra Cahyaningtyas(2), Adi Imam Cahyadi(3), Hesti Lina Wiraswati(4), Julia Ramadhanti(5)

(1) Divisi Mikrobiologi, Departemen Ilmu Kedokteran Dasar, Program Studi Kedokteran Hewan, Fakultas Kedokteran, Universitas Padjadjaran, Bandung, Indonesia
(2) Prodi Kedokteran Hewan Fakultas Kedokteran Universitas Padjadjaran
(3) Departemen Ilmu Kedokteran Dasar Divisi Mikrobiologi Fakultas Kedokteran Universitas Padjadjaran
(4) Departemen Ilmu Kedokteran Dasar Divisi Parasitologi Fakultas Kedokteran Universitas Padjadjaran
(5) Departemen Ilmu Kedokteran Dasar Divisi Farmakologi Fakultas Kedokteran Universitas Padjadjaran
(*) Corresponding Author

Abstract


Staphylococcus aureus adalah bakteri coccus Gram positif dari keluarga staphylococcaceae sebagai bakteri utama penyebab mastitis subklinis. Tujuan penelitian adalah untuk melakukan identifikasi bakteri Staphylococcus aureus sebagai penyebab mastitis subklinis pada sapi perah dengan menggunakan metode Polymerase Chain Reaction (PCR). Sampel penelitian berupa 38 isolat Staphylococcus aureus yang telah diisolasi dari sampel susu sapi perah penderita mastitis subklinis pada penelitian sebelumnya di Peternakan Mekar Bakti dan Peternakan Putra Saluyu, Kecamatan Pamulihan, Kabupaten Sumedang. Hasil identifikasi fenotipik isolat Staphylococcus aureus yang dikultur pada media Blood Plate Agar (BAP) menunjukkan sebanyak 10 isolat (26%) bersifat α-hemolisis, 28 isolat (74%) bersifat β-hemolisis. Pewarnaan Gram pada 38 isolat (100%) menunjukkan koloni bersifat gram positif dengan bentuk koloni coccus bergerombol. Hasil uji katalase dan koagulase pada 38 isolat (100%) menunjukkan hasil positif. Hasil uji DNase pada 29 isolat (76%) bereaksi positif, sedangkan sebanyak 9 isolat (24%) menunjukkan hasil negatif. Identifikasi secara genotipik dilakukan berdasarkan amplifikasi terhadap gen 23S rRNA, gen nuc, dan gen coa. Hasil penelitian menunjukkan bahwa 38 isolat (100%) teridentifikasi sebagai Staphylococcus aureus yang merupakan bakteri penyebab mastitis subklinis pada sapi perah di Wilayah Kabupaten Sumedang

Keywords


Sapi perah; Mastitis Subklinis; PCR; Staphylococcus aureus

Full Text:

PDF


References

Abbas, B. A., Khudor, M. H. & Hanoon, B. M. (2014). Isolation and identification of Staphylococcus aureus from bovine and the detection of its coagulase gene (coa) using polymerase chain reaction (PCR). Sci. Res. Essays, 9(20): 864-868. Alhussien, M.N., & Dang, A.K. (2020). Sensitive and rapid lateral-flow assay for early detection of subclinical mammary infection in dairy cows. Sci Rep 10, 11161. https://doi.org/10.1038/s41598- 020-68174-0 Aziz, F., Lestari, F. B., Nuraida, S. S., Purwati, E., & Salasia, S. I. O. (2020). Deteksi Staphylococcus aureus dan Staphylococcus sp. secara Langsung dari Susu Segar Kambing Peranakan Etawa dengan Polymerase Chain Reaction (PCR). Jurnal Sain Veteriner. Vol. 38 No. 2, 168-174. Brakstad, O. G., Aasbakk, K., & Maeland, J. A. (1992). Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. Journal of clinical microbiology, 30(7), 1654–1660. https://doi.org/10.1128/jcm.30.7.1654-1660.1992 Cheng, W. N., & Han, S. G. (2020). Bovine mastitis: risk factors, therapeutic strategies, and alternative treatments - A review. Asian-Australasian journal of animal sciences, 33(11), 1699–1713. https://doi.org/10.5713/ajas.20.0156 Cotar, A. I., Chifiriuc, M. C., Dinu, S., Bucur, M., Iordache, C., Banu, O., Dracea, O., Larion, C., & Lazar, V. (2010). Screening of molecular virulence markers in S. aureus and Pseudomonas aeruginosa strains isolated from clinical infections. Int. J. Mol. Sci. 11(12), 5273–5291. Effendi, M. H. & Harijani, N. (2017). Cases of Methicillinresistant Staphylococcus aureus (MRSA) from raw milk in East Java, Indonesia. Glob. Vet., 19(1): 500-503. Effendi, M. H., Hisyam, M. A. M., Hastutiek, P., & Tyasningsih, W. (2019). Detection of coagulase gene in Staphylococcus aureus from several dairy farms in East Java, Indonesia, by polymerase chain reaction. Veterinary World, 12(1), 68-71. https://doi.org/10.14202/vetworld.2019.68-71 Errata. (2012). Catalase-negative Staphylococcus aureus isolated from a diabetic foot ulcer. Iranian Journal of Microbiology, 4(2), 101. Foster, T.J. and Geoghegan, J.A. (2015). Chapter 37: Staphylococcus aureus. In: Tang, Y.W., Sussman, M., Liu, D., Poxton, I. and Schwartzman, J., editors. Molecular Medical Microbiology. 2nd ed. Academic Press, Boston. p655-674. Gomes, F., & Henriques, M. (2016). Control of Bovine Mastitis: Old and Recent Therapeutic Approaches. Current microbiology, 72(4), 377–382. https://doi.org/10.1007/s00284-015-0958-8 Hayati, L. N., Tyasningsih, W., Praja, R. N., Chusniati, S., Yunita, M. N., & Wibawati, P. A. (2019). Isolasi dan Identifikasi Staphylococcus aureus pada Susu Kambing Peranakan Etawah Penderita Mastitis Subklinis di Kelurahan Kalipuro, Banyuwangi. Jurnal Medik Veteriner, 2(2), 76-82. DOI: 10.20473/jmv.vol2.iss2.2019.76-82 Hookey, J. V., Richardson, J. F., & Cookson, B. D. (1998). Molecular typing of Staphylococcus aureus based on PCR restriction fragment length polymorphism and DNA sequence analysis of the coagulase gene. Journal of clinical microbiology, 36(4), 1083–1089. https://doi.org/10.1128/JCM.36.4.1083-1089.1998 Hoque, M. N., Das, Z. C., Rahman, A., Haider, M. G., & Islam, M. A. (2018). Molecular characterization of Staphylococcus aureus strains in bovine mastitis milk in Bangladesh. International journal of veterinary science and medicine, 6(1), 53–60. https://doi.org/10.1016/j.ijvsm.2018.03.008 Husna, C. A. (2018). Peranan Protein Adhesi Matriks Ekstraselular dalam Patogenitas Bakteri Staphylococcus aureus. Jurnal Averrous Vol. 4 No. 2. Iraguha, B., Hamudikuwanda, H., Mushonga, B., Kandiwa, E., & Mpatswenumugabo, J. P. (2017). Comparison of Cow-side Diagnostic Tests for Subclinical Mastitis of Dairy Cows in Musanze district, Rwanda. Journal of the South African Veterinary Association, 88(1), 1–6. https://doi.org/10.4102/jsava.v88i0.1464 Javid, F., Taku, A., Bhat, M. A., Badroo, G. A., Mudasir, M., & Sofi, T. A. (2018). Molecular typing of Staphylococcus aureus based on coagulase gene. Veterinary world, 11(4), 423–430. https://doi.org/10.14202/vetworld.2018.423-430 Jiwintarum, Y., Srigede, L., & Rahmawati, A. (2015). Perbedaan Hasil Uji Koagulase Menggunakan Plasma Sitrat Manusia 3,8%, Plasma Sitrat Domba 3,8%, dan Plasma Sitrat Kelinci 3,8% pada Bakteri Staphylococcus aureus. Jurnal Kesehatan Prima, Vol. 9 No.2, 1559-1569. Karimela, E. J., Ijong, F. G., & Dien, H. A. (2017). Karakteristik Staphylococcus aureus yang diisolasi dari ikan asap pinekuhe hasil olahan tradisional Kabupaten Sangihe. Jurnal Pengolahan Hasil Perikanan Indonesia. 20(1): 188-198. Kou X, Cai H, Huang S, Ni Y, Luo B, Qian H, Ji H, & Wang X. (2021). Prevalence and Characteristics of Staphylococcus aureus Isolated From Retail Raw Milk in Northern Xinjiang, China. Front. Microbiol. 12: 705947. doi: 10.3389/fmicb.2021.705947 Koupahi, H., Honarmand Jahromy, S., & Rahbar, M. (2016). Evaluation of Different Phenotypic and Genotypic Methods for Detection of Methicillin Resistant Staphylococcus aureus(MRSA). Iranian journal of pathology, 11(4), 370–376. Moraveji, Z., Tabatabaei, M., Shirzad Aski, H., & Khoshbakht, R. (2014). Characterization of hemolysins of Staphylococcus strains isolated from human and bovine, southern Iran. Iranian journal of veterinary research, 15(4), 326–330. Nawar, S., Rashid, M., Ahmed, A., Hossain, M., & Afzal, A. (2021). A Study of Prevalence and Pathogenic Activity of Bacteria in the Air of Dhaka City and Their Antimicrobial Resistance Pattern. American Journal of Molecular Biology, 11, 51-62. doi: 10.4236/ajmb.2021.112005. Nisa, H. C., Purnomo, B. S., Damayanti, T. L., Hariadi, M., Sidik, R., & Harijani, N. (2019). Analisis Faktor yang Mempengaruhi Kejadian Mastitis Subklinis dan Klinis pada Sapi Perah. Ovozoa, Vol. 8 No. 1, 66-70. ISSN: 2302-6464 Prasetyo, B. dan Kusumaningrum, E. N. (2014). Deteksi Gen tst Isolat Staphylococcus aureus melalui Amplifikasi 23S rRNA Asal Susu Kambing dan Sapi Perah. Jurnal Kedokteran Hewan. Vol. 8 No. 1. 76 – 79. Pumipuntu, N., Kulpeanprasit, S., Santajit, S., Tunyong, W., Kong-Ngoen, T., Hinthong, W., & Indrawattana, N. (2017). Screening method for Staphylococcus aureus identification in subclinical bovine mastitis from dairy farms. Veterinary world, 10(7), 721–726. Sizar O & Unakal C. G. (2022). Gram Positive Bacteria. [Updated 2022 Feb 14]. In: StatPearls [Internet]. Treasure Island (FL): StatPearls Publishing; 2022 Jan-. Available from: https://www.ncbi.nlm.nih.gov/books/NBK470553/ Straub, J. A., Hertel, C. and Hammes, W. P. (1999). A 23S rDNA-Targeted Polymerase Chain ReactionBased System for Detection of Staphylococcus aureus in Meat Starter Cultures and Dairy Products. J. Food Prot. 62 (10):1150–1156 Susanty, H. (2018). Evaluasi Penerapan Aspek Teknis dan Sebaran Spasial Produksi Susu dan Prevalensi Mastitis Subklinis Sapi Perah Rakyat di Jawa Barat (Doctoral dissertation, IPB (Bogor Agricultural University)). Sutejo, S. V. H., Amarantini, C., & Budiarso, T. Y. (2017). Molecular Detection of Staphylococcus aureus Resistant to Temperature in Milk and Its Products. 8th International Conference on Global Resource Conservation (ICGRC 2017). AIP Conf. Proc. 1908, 050007-1–050007-6; https://doi.org/10.1063/1.5012731 Toelle, N. N. dan Lenda, V. (2014). Identifikasi dan Karakteristik Staphylococcus Sp. dan Streptococcus Sp. dari Infeksi Ovarium Pada Ayam Petelur Komersial. Jurnal Ilmu Ternak, Vol. 1, No. 7, 32 - 37. Wang, D., Zhang, L., Zhou, X., He, Y., Yong, C., Shen, M., & Han, B. (2016). Antimicrobial susceptibility, virulence genes, and randomly amplified polymorphic DNA analysis of Staphylococcus aureus recovered from bovine mastitis in Ningxia, China. Journal of Dairy Science, 99(12): 9560–9569. Windria, S., Widianingrum, D. C., & Salasia, S. I. O. (2016). Identification of Staphylococcus aureus and coagulase negative staphylococci isolates from mastitis milk of etawa crossbred goat. Research Journal of microbiology, 11(1), 11. Windria, S., Wiraswati, H. L., Ramadhanti, J., Tyas, T. K. A., & Wismandanu, O. (2018). Penyuluhan Mastitis Subklinis pada Sapi Perah di Desa Mekar Bakti Kecamatan Pamulihan Kabupaten Sumedang Jawa Barat. Dharmakarya: Jurnal Aplikasi Ipteks untuk Masyarakat. Vol. 7, No. 2: 138 - 140



DOI: https://doi.org/10.22146/jsv.76052

Article Metrics

Abstract views : 734 | views : 1263

Refbacks

  • There are currently no refbacks.




Copyright (c) 2023 Jurnal Sain Veteriner

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Sain Veteriner Indexed by

    CrossrefROADCOREProduct DetailsDESKRIPSI GAMBAR


Copyright of JSV (Jurnal Sain Veteriner) ISSN 0126-0421 (print), ISSN 2407-3733 (online).

Fakultas Kedokteran Hewan, Universitas Gadjah Mada

Jl. Fauna No.2, Karangmalang, Yogyakarta

Phone: 0274-560862

Fax: 0274-560861

Email: jsv_fkh@ugm.ac.id

HP. 0895363078367

Jurnal Sain Veteriner is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

 

free
web stats View My Stats