Potensi Tepung Magot Black Soldier Fly (Hermetia illucens) sebagai Agen Antibakteri dan Immunomodulator Pakan Ternak Unggas secara In vitro

https://doi.org/10.22146/jsv.53347

Desy Cahya Widianingrum(1*), Melinda Erdya Krismaputri(2), Listya Purnamasari(3)

(1) Department of Animal Husbandry, Faculty of Agriculture, Jember University
(2) Department of Animal Husbandry, Faculty of Agriculture, Jember University
(3) Department of Animal Husbandry, Faculty of Agriculture, Jember University
(*) Corresponding Author

Abstract


Tepung Magot dalam pakan unggas tidak hanya dapat digunakan sebagai alternatif sumber protein namun juga diharapkan memiliki efek antibakterial dan immunomodulator. Penelitian ini menggunakan metode in vitro untuk mengetahui efek antimikrobial dan immunomodulator tepung magot. Uji sensitivitas dilakukan dengan metode disc difusi agar, uji aktivitas fagositosis diamati pada makrofag peritoneum mencit Balb-C jantan berumur 8 minggu terhadap Staphylococcus aureus (S. aureus), serta uji tantang S. aureus terhadap tepung magot dilihat di bawah scanning electron microscopy (SEM). Data hasil uji sensitivitas dan pengamatan dengan teknik SEM dilaporkan secara deskriptif. Perbedaan aktivitas fagositosis makrofag antar perlakuan diuji dengan analisis varian satu arah dengan uji lanjut honestly significant difference (HSD) Berdasar hasil penelitian diketahui bahwa tepung memiliki 38,22% kandungan protein dengan profil asam amino yang lengkap. Kandungan asam amino tertinggi pada tepung magot adalah (7685,84 mg/kg), aspartat (5864,19 mg/kg), leusin (5034,31 mg/kg). Asam lemak esensial yang terkandung pada tepung magot adalah asam laurat (13,39%) Hasil uji sensitivitas diketahui tepung magot tidak memberikan zona hambat pada bakteri S. aureus. Introduksi tepung magot pada fagositosis secara in vitro dapat meningkatkan kinerja makrofag dengan perannya seperti opsonin berdasar pengamatan SEM. Kesimpulan dari penelitian ini adalah tepung magot potensial digunakan sebagai imunomodulator natural dan pengganti protein pakan unggas.

Keywords


antibakterial, immunomodulator, pakan unggas, tepung magot

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References

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DOI: https://doi.org/10.22146/jsv.53347

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