Prevalensi Strain Avian Pathogenic Escherichia coli (APEC) Penyebab Kolibasilosis pada Burung Puyuh

Wahyu Prihtiyantoro(1*), Khusnan Khusnan(2), Mitra Slipranata(3), Imron Rosyidi(4)

(1) Akademi Peternakan Brahmaputra, Yogyakarta
(2) Akademi Peternakan Brahmaputra, Yogyakarta
(3) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(4) Fakultas Kedokteran Hewan, Universitas Gadjah Mada
(*) Corresponding Author


Avian Pathogenic Escherichia coli (APEC) is a pathogen that causes colibacillosis in poultry, including salpingitis, omphalitis, cellulitis, swollen head syndrome, coligranuloma yolk sac inflammation, and air sacs inflammation. APEC is a zoonotic strain which spread through raw meat and processed meat products of animals and birds. In this research, the isolation and identification of Escherichia coli were done by using selective media MacConkey, Kligger Iron Agar, and Gram staining. Polymerase chain reaction (PCR) was used to analyse genetopically to detect 16SrRNA genes, vt1 genes, and vt2 genes. Thirty one (55,36%) isolates of 56 specimens collected from quail were detected as Escherichia coli. The detection of APEC strains towards 31 Escherichia coli isolates were done by using polymerase chain reaction (PCR) with vt1 and vt2 specific primer. The results showed that 32,26% (10/31) was APEC strains and 67.74% was non-APEC strains. From 10 isolates, 90% had vt1 gene and 10% had vt2 gene. Escherichia coli isolates were found in eyes (32,26%), infraorbital sinus fluid (32,26%), nasal fluid (16,20%), also in lungs, air sacs, ascites, and heart for 3,2% each. The isolates could not be found in the specimens from the skull. As a zoonotic agent, the isolates have an impact on human health. 


APEC; colibacillosis; quail; vt1; vt2

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Afroz H., Sultana F., Fakruddin M., Khan Z.U.M. and Datta S. (2013) Isolation of Escherichia coli and Staphylococcus aureus from Full Cream Powder Milk Sold Under Market Conditions at Dhaka, Bangladesh and Their Antibiotic Susceptibility. J. Adv. Sci. Res.


Aidar-Ugrinovich L., Blanco J., Blanco M., Blanco J.E., Leomil L., Dahbi G., Mora A., Onuma D.L., Silveira W.D. and de Castro P.A.F. (2007). Serotypes, virulence genes, and intimin types of Shiga toxin-producing Escherichia coli (STEC) and enteropathogenic E. coli (EPEC) isolated from calves in Sao Paulo, Brazil. Int. J. Food. Microbiol. 115:297-306.

Akbar H., Khan M., Khan A.A., Khan M.A., Shuaib M., Akbar S.F., Manzoor S., Irshadurrehman S., Ahmad L., Ali R., Khalid R. and Idrees M. (2009) Comparative efficacy of doxycycline and flumequine against experimentally induced colibacillosis in broiler chicks. J. of Vet. Med. and Anim. Health. 1 (2):17-22.

Alonso M.Z., Krüger A., Sanz M.E., Padola N.L. and Lucchesi P.M.A. (2016) Serotypes, virulence profiles and stx subtypes of Shigatoxigenic Escherichia coli isolated from chicken derived products. Rev. Argent. Microbiol. 48(4):325-328.

Anonimus (2016) Instructions for use Kligger Iron A g a r . rod/Content/hugo/ m

Antao EM., Glodde S., Li G., Sharifi R., Homeier T. and Laturnus C. (2008). Thechicken as anatural model for extra intestinal infections caused by avian pathogenic Escherichia coli (APEC). Microbiol. Pathol. 45:361-369.

Arenas A, Vicente S. and Luque I. (1999). Outbreak of septicaemic colibacillosis in Japanese quail (Coturnix coturnix japonica). Zentralbl Veterinarmed B. 46:399-404.

Barbieri N.L., de Oliveira A.L., Tejkowski T.M., Pavanelo D.B., Rocha D.A., Matter L.B., Callegari-Jacques S.M., de Brito B.G., Horn F. (2013) Genotypes and pathogenicity of cellulitis isolates reveal traits that modulate APEC virulence.

Barnes H.J, Vaillancourt J.P. and Gross W.B. (2003). Newcastle disease, p.631-656. In: Ibid. (Ed.), Diseases of Poultry. 11th ed. Iowa State University Press, Ames.

Barnes H.J., Vaillancourt J.P. and Gross R.G. (1997). Colibacillosis. In: Y. M. Saif, H. J. Barnes, J. R. Glisson, A. M. Fadly, L.R. MacDougal, and D. E. Swayne. Diseases of Poultry, 11thEd. Iowa State University Press, Iowa, USA. 631-652.

Bilge S.S., Clausen C.R., Lau W. and Moseley S.L. (1989) Molecular characterization of a fimbrial adhesin, F1845, mediating diffuse adherence of diarrhea-associated Escherichia coli to HEp-2 cells. J. Bacteriol 171:4281-4289.

Bottero M.T., A. Dalmasso, D. Soglia, S. Rosati, L. Decastelli, and T. Civera. 2004. Development of a multiplex PCR assay for the identification of pathogenic genes of Escherichia coli in milk and milk products. Mol. Cell.Probes. 18(4):283-288.

Calnek B.W., Barnes H.J., Beard C.W., McDougald L.R. and Saif Y.M. (1997). Diseases of Poultry. 10th edition. Iowa State University Press, Ames, Iowa. 131-140.

Carson C.A., Shear B.L., Ellersieck M.R. and Asfaw A. (2001) Identification of Fecal Escherichia coli from Humans and Animals by Ribotyping Appl. Environ. Microbiol. 67(4):1503-1507.

Dho-Moulin M. and Fairbrother J.M. (1999). Avian pathogenic Escherichia coli (APEC). Vet. Res. 30:299-316.

Dı'az-Sa'nchez S., Sa´nchez S., Ewers C. and Hofle U. (2012). Occurrence of avian pathogenic Escherichia coli and antimicrobialresistant E. coli in red-legged partridges (Alectoris rufa): sanitary concerns of farming. Avian Pathol. 41(4):337-344.

El Tawab A.A., El Aal S.A.A., Mazied E.M. and Morsy D.A. (2015) Prevalence of E. coli in broiler chickens in winter and summer seasons by application of PCR with its antibiogram pattern. Benha Vet. Med. J. 29(2):119-128.

El-Hewairy H.M., Awad W.S. and Ibrahim A.K. (2009) Serotyping and molecular characterization of Escherichia coli isolated from diarrheic and in-contact camel calves. Egypt. J. Comp. Path. and Clinic. Path. 22(1): 216-233 Elsayed M.E., Shabana I.I., Esawy A.M. and Rashed A.M. (2015) Detection of Virulence Associated Genes of Avian Pathogenic Escherichia coli (APEC) Isolated from Broilers. J.J. Genetics.1(1): 004. s/Genetics/J_J_Gene_1_1_004.pdf

Emery D.A., Nagaraja K.V., Shaw D.P., Newman J.A. and White D.G. (1992) Virulence factors of Escherichia coli associated with colisepticemia in chickens and turkeys. Avian Dis. 36: 504-511.

Hamad M.A., Al-Aalim A.M., Al-Dabbagh S.Y.A. and Ali H.H. (2012) Detection of organ bacterial load in quails. Iraqi J of Vet Scie. 26:47-51

Hasan A.K.M.R., Ali M.H., Siddique M.P., Rahman M.M. and Islam M.A. (2010) Clinical and Laboratory Diagnoses of Common Bacterial Diseases of Broiler and Layer Chickens. Bangl. J. Vet. Med. 8(2):107-115.

HemmatinezhadB., Khamesipour F., Mohammadi M., Dehkordi F.S. and Mashak Z. (2015) Microbiological Investigation of OSerogroups, Virulence Factors and Antimicrobial Resistance Properties of Shiga Toxin-Producing Escherichia coli Isolated from Ostrich, Turkey and Quail Meats. J. of food savety. doi: 10.1111/jfs.12199. 1-10

Hussein A.H., Ghanem I.A., Eid A.A., Ali M.A., Sherwood J.S., Li G., Nolan, L.K. and Logue C.M. (2013) Molecular and phenotypic characterization of Escherichia coli isolated from broiler chicken flocks in Egypt. Avian Dis., 57(3):602–611.

Ito H., Skoda S., Kobayashi S., Sugiyama H. and Masanori N. (1990) Colibacillosis of Japanese quail (Coturnix coturnix japonica) occurring in Higashimikawa District. J. Jpn. Vet. Med. Assoc. 43: 661– 665.

Jakaria A.T.M., Islam M.A. and Khatun M.M. (2012). Prevalence, characteristics and antibiogram profiles of Escherichia coli isolated from apparently healthy chickens in Mymensingh, Bangladesh. Microbes and Health 1: 27-29

Kabir S.M.L. (2010) Avian Colibacillosis and Salmonellosis: A Closer Look at Epidemiology, Pathogenesis, Diagnosis, Control and Public Health Concerns. Int. J. Environ. Res. Public Health. 7:89-114.

Kalin R., Ongor H. and Cetinkaya B. (2012) Isolation and molecular characterization of Escherichia coli O157 from broiler and human samples. Foodborne Pathog. Dis. 9(4):313-318.

Knöbl T., Tania A., Gomes, T., Vieira M.A.M., Jose A., Bottinoa J.A., Antonio J. and Ferreira P. (2004) Detection of pap, sfa, afa and fim adhesin-encoding operons in avian pathogenic Escherichia coli. Intern J. Appl. Res. Vet. Med. 2(2):135-141.

Law D. (2000) Virulence factors of Escherichia coli O157 and other Shiga toxin-producing E. coli. J. of Appl Microbiol. 88:729-745.

Lee K., French N.P., Hara-Kudo Y., Iyoda S., Kobayashi H., Sugita- Konishi Y., Tsubone H. and Kumagai S. (2011) Multivariate analyses revealed distinctive features differentiating human and cattle isolates of Shiga toxinproducing Escherichia coli O157 in Japan. J. Clin. Microbiol. 49: 1495-1500.

Lowy F. (2009) Bacterial Classification, Structure and F u n c t i o n . thophys/id/2009/introNotes.pdf

Ludwig K., Sarkim V., Bitzan M., Karmali M.A., Bobrowski C. and Ruder H. (2002) Shiga Toxin-Producing Escherichia coli infection and antibodies against stx2 and stx1 in household contacts of children with enteropathic hemolytic-uremic syndrome. J. Clin. Microbiol. 40:12-17.

Mamun M.M., Parvej M.S., Ahamed S., Hassan J., Nazir K.H.M.N.H., Nishikawa Y. and Rahman M.T. (2016) Prevalence And Characterization of Shigatoxigenic Escherichia coli in Broiler Birds in Mymensingh. Bangl. J. Vet. Med. 14(1):5-8.

Manoj Kr. Saikia and Saikia D. (2011) PCR Detection of stx1 and stx2 Toxigenic Genes in Multiple. Antibiotic Resistant Escherichia coli Population and Phenotypic Detection of Esbl Producing Escherichia coli. Isolates from Local Variety of Poultry. Int. J. of Appl. Biol. and Pharmaceut. Technol. 3(2):593602.

Matter L.B., Barbieri N.L., Nordhoff M., Ewers C. and Horn F. (2011) Avian pathogenic Escherichia coli MT78 invades chicken fibroblasts Vet. Microbiol.148:51-59.

McPeake S.J.W., Smyth J.A. and Ball H.J. (2005) Characterisation of Avian Pathogenic Escherichia coli (APEC) associated with colisepticaemia compared to faecal isolates from healthy birds. Vet. Microbiol. 110: 245-253.

Mitra M., Pramanik A.K., Bhattacharyya H.M., Basak D.K. and Chatterjee A. (2004) Spontaneous colibacillosis in infectious bursal diseaseaffected broiler flocks. Trop. Anim. Health. Prod. 36(7):627-632.

Mohamed M.A., Shehata M.A. and Rafeek E. (2014) Virulence Genes Content and Antimicrobial Resistance in Escherichia coli from Broiler Chickens. Vet. Med. Int. Article ID 195189, 6 page 14/195189

Monroy M.A., Knobl T., Bottino J.A., Ferreira C.S. and Ferreira A.J.P. (2005) Virulence characteristics of Escherichia coli isolates obtained from broilers breeders with salpingitis. Comp. Immunol. Microbiol. Infect. Dis. 28:1-15.

Moon B.M., Won G.Y., Choi Y.Y., Jin J.K., Oh I.G., Park J.H., Eo S.K. and Lee J.H. (2006) Isolation and characteristics of avian pathogenic Escherichia coli from birds associated with colibacillosis. Proceedings of AZWMP 2006 Chulalongkorn Uni. Fac. of Vet. Sc., Bangkok, Thailand, 26-29 Oct 2006: 61

Nakazato G., Campos T.A., Stehling E.G., Brocchi M. and Da Silveira W.D. (2009) Virulence factors of avian pathogenic Escherichia coli (APEC). Pesq. Vet. Bras. 29:479-486.

Nataro J.P. and Kaper J.B. (1998) Diarrheagenic 17. Escherichia coli. Clin. Microbiol Rev. 11:142-201. Naylor S.W., Gally D.L. and Low J.C. (2005) Enterohaemorrhagic E. coli in veterinary medicine. Int. J. Med. Microbiol. 295:419441.

Oh J.Y., Kang M.S., Kim J.M., An B.K., Song E.A., Kim J.Y., Shin E.G., Kwon K.J.H. and Kwon Y.K. (2011) Characterization of Escherichia coli isolates from laying hens with colibacillosis on 2 commercial eggproducing farms in Korea. Poul. Sci. 90:1948–1954.

Radwan I.A., Salam H.H.S., Abd-Alwanis S.A.A. and Al-Sayed M.A.Y. (2014) Frequency of some virulence associated genes among multidrug-resistant Escherichia coli isolated from septicemic broiler chicken. Int J of Adv Res. 2(12):867-874.

Ramirez R.M., Almanza Y., Gonzalez R., Garcia S. and Heredia N. (2009) Avian pathogenic Escherichia coli bind fibronectin and laminin. Vet. Res. Comm. 33:379-386.

Rehman M.U., Rashid M., Sheikh J.A. and Bhat M.A. (2014). Molecular epidemiology and antibiotic resistance pattern of Enteropathogenic Escherichia coli isolated from bovines and their handlers in Jammu, India. J. of Adv. Vet. and Anim. Res. 1:177181.

Rodriguez-Siek K.E., Giddings C.W., Doetkott C., Johnson T.J., and Nolan, L.K. (2005) Characterizing the APEC pathotype. Vet. Res. 36:241–256.

Roy P., Edwin P.G. and Pursushothaman V. (2004) Characterization of Escherichia coli isolates from hatchery and breeder hens. Indian Vet. J. 81:1317-1320.

Roy P., Purushothman V., Kotesswaran A. and Dhillon P. (2006) Isolation, Characterization and Antibimicrobial Drug Resistance Pettern of Eschrichia coli Isolated from Japanese Quail and their Environment. J. Af App Poult Res. 15:442-446.

Roy SR., Rahman M.B., Hassan J. and Nazir K.H.M.N.H. (2012). Isolation and identification of bacterial flora from internal organs of broiler and their antibiogram studies. Microbes and Health 1:72-78.

Saif Y.M., Fadly A.M., Glisson J.R., McDougald L.R., Nolan L.K. and Swayne, D.E. (2008) Diseases of poultry. 12th ed. Blackwell Publishing, London. Salehi T.Z., Safarchi A., Peighambari S.M., Mahzounieh M. and Khorasgani R.M. (2007) Detection of stx1, stx2, eae, espB and hly genes in avian pathogenic Escherichia coli by multiplex polymerase chain reaction. J. Vet. Res. 62(2):37-42.

Salehi Z., Yahya T. and Raeyat R. (2001) Serotyping of isolated Escherichia coli from poultry in Tehran province. J. Fac. Vet. Med. Univ. Tehran. 56:17-20. Salehi, M. and Ghanbarpour, R. (2010) Phenotypic and genotypic properties of Escherichia coli isolated from colisepticemic cases of Japanese quail. Trop An. Health and Product. 42(7):1497-1504.

Sarah A.Y., Ammar A.M. and Ahmed D.A. (2015) Serological and Molecular Typing of Avian Pathogenic E. coli Originating from Outbreaks of Colibacillosis in Chicken Flocks. Int. J. of Sci. and Res. (IJSR) 4(2): 2062-2088.

Sepehri G. and Zadeh A.H. (2006) Prevalence of bacterial resistance to commonly used antimicrobials among Escherichia coli isolated from chickens in Kerman Province of Iran. J. Med. Sci. Pakistan. 6(1):99-102.

Soon-Gu K., Se-Yeoun C., Eun-Ju C., Kim B., HeeJong S. and Hyung-Kwan J. (2008) Epidemiological Prevalence of Avian

Pathogenic Escherichia coli Differentiated by Multiplex PCR from Commercial Chickens and Hatchery in Korea. J of Bacteriol and Virol. 38(4):179-188.

Suryani A.E., Karimy M.F., Istiqomah L., Sofyan A., Herdian H. and Wibowo M.H. (2014) Colibacillosis Prevalence In Broiler Chicken Infected By Escherichia coli With Administration of Bioadditive. Probiotic and Antibiotic Widyariset, 17(2):233–244.

Talebiyan R., Kheradmand M., Khamesipour F. and Rabiee-Faradonbeh M. (2014) Multiple Antimicrobial Resistance of Escherichia coli Isolated from Chickens in Iran. H i n d a w i P u b l i s h i n g Corporation.Veterinary Medicine International Article ID 491418, 4 pages

Zinnah M.A., Bari M.R., Islam M.T., Hossain M.T., Rahman M.T., Haque M.H., Babu S.A.M., Ruma R.P. and Islam M.A. (2007) Characterization of Escherichia coli Isolated from Samples of Different Biological and Environmental Sources. Bangl. J. Vet. Med. 5 (1 and 2):25–32.


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