Profil Darah dan Gambaran Makroskopis Kesembuhan Luka Tikus Wistar  (Rattus norvegicus) Infeksi Staphylococcus aureus yang  Diberi Ekstrak Bawang Merah (Allium cepa L.)

Imron Rosyadi; Desriza Alhidayany; Christin Marganingsih S

doi:10.22146/jsv.44891

Profil Darah dan Gambaran Makroskopis Kesembuhan Luka Tikus Wistar (Rattus norvegicus) Infeksi Staphylococcus aureus yang Diberi Ekstrak Bawang Merah (Allium cepa L.)

https://doi.org/10.22146/jsv.44891

Imron Rosyadi^(1*), Desriza Alhidayany⁽²⁾, Christin Marganingsih S⁽³⁾

(1) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(2) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(3) Fakultas Kedokteran Hewan Universitas Gadjah Mada
(*) Corresponding Author

Abstract

Wound is one of the most common cases happened frequently. Infected wound by pathogenic microorganism could inhibit wound healing process. The microorganism that often infect the wound is Staphylococcus aureus. Shallot had believed as one of traditional medicine that believes as an alternative therapy for wound healing. This study aimed to determine the blood profile and macroscopic images of wound healing in Wistar rats (Rattus norvegicus) infected by Staphylococcus aureus and given shallot extract (Allium cepa L.). This study used 12 rats, aged about 2 months and weighed 180-250 gram is divided into 4 groups, each group consist of 3 rats. Rats were injured by incision wound at 1 cm length and ± 2 mm depth, then infected by Staphylococcus aureus bacteria. Group I and II were droped of shallot extract 40% and 80%, while Group III was droped by povidon iodine 10%, and Group IV was given nothing (control group) until the wound healed. Research observation was did at day-0, 7, 14, and 21. The length of wound was measured in centimeter evere week and blood sampling were done at day-0 and day-21. The observational data of wound length were analysis using Friedman, Wilcoxon, dan Mann-Whitney. The result of research showed the wound length average on day-7 in Group I: 0,733 cm; Group II: 0,433 cm; Group III: 0,567 cm; and Group IV: 0,767 cm (p<0,05). The wound length average on day-14 in Group I: 0,433 cm and Group IV: 0,333 cm (p>0,05), Group II and III were healed (p>0,05). In day-21 all wound were completely healed. The rats blood profile on day-0 and day-21 showed that erythrocytes total count, hemoglobin (Hb), and packed cell volume (PCV) were in normal range. Rats were in health condition. Thus it could be concluded that the time need for the wounds to heal in rats that were treated with shallot extract 80% is equal to rats were treated with povidon iodine 10% on the 14^th day of the study while rats that were treated with shallot extract 40% is equal to rats was given nothing on the 21^st day of the study. Shallot extract 80% can treat wound that infected by Staphylococcus aureus is equal to povidon iodine 10% treatment.

Keywords

Wound; Staphylococcus aureus; shallot extract

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References

Akbar, B. 2010. Tumbuhan dengan Kandungan Senyawa Aktif yang Berpotensi sebagai Bahan Antifertilitas. Penerbit Adabia Press, Jakarta, 4. Arifin, H., Anggraini, N., Handayani, D., dan Rasyid, R. 2006. Standarisasi Ekstrak Etanol Daun Eugenia Cumini Merr. Jurnal Sains Teknologi Farmasi 11 (2): 88-93. Baradero, M., Dayrit, M. W., dan Siswadi, Y. 2008. Prinsip dan Praktik Keperawatan Perioperatif. EGC, Jakarta, 110. Benjamin, M. M. 1962. Outline of Veterinary Clinical Pathology 2nd Edition. The Iowa State University Press, USA, 38, 56. Brooks, G.F., Carrol, K.C., Butel, J.S., Morse, S.A., and Mietzner, T.A. 2012. Jawets, Melnick, & Adelberg’s Mikrobiologi Kedokteran Edisi 25. Penerjemah: Nugroho, A.W., dkk. Judul buku asli : Jawets, Melnick, & Adelberg’s Medical Microbiology. EGC, Jakarta, 194-198. Brown, R. G., and Burns, T. 2005. Lecture Notes on Dermatologi Edisi Kedelapan. Penerjemah: Zakaria, A. Judul asli: Lecture Notes on Dermatology Eighth Edition. Erlangga, Jakarta, 8. Cheesbrough, M. 2006. District Laboratory Practice in Tropical Country. Cambridge University Press, UK, 309. Coles, E. H. 1974. Veterinary Clinical Pathology. W. B. Sounders Company, London, 101-102. Depkes RI. 2000. Inventaris Tanaman Obat Indonesia (I) Jilid 1. Departemen Kesehatan RI dan Kesejahteraan Sosial RI Badan Penelitian dan Pengembangan Kesehatan, Jakarta, 13. Dewi, A.K. 2013. Isolasi, Identifikasi dan Uji Sensitivitas Staphylococcus aureus terhadap Amoxicillin dari Sampel Susu Kambing Peranakan Ettawa (PE) Penderita Mastitis di Wilayah Girimulyo, Kulonprogo, Jurnal Sain Veteriner, Yogyakarta, 31 (2): 138-150. Drosou, A.M.D., Falabella, A.M.D., and Kirsner, R.S.M.D. 2003. Antiseptics on Wounds: An Area of Controversy. (online). http.medscape.com/viewarticle/456300 [6 Februari 2017]. Dukes, H. H. 1947. The Phisiology of Domesic Animals 6th Edition. Comstock Publishing Company Ithaca, New York, 36. Fitria, L., and Sarto, M. 2014. Profil Hematologi Tikus (Rattus norvegicus Berkenhout, 1769) Galur Wistar Jantan dan Betina Umur 4,6, dan 8 Minggu. Jurnal Ilmiah Biologi 2 (2): 94-100. Hidayat, R.S., Napitupulu, R.M., 2015. Kitab Tumbuhan Obat. Agriflo, Jakarta, 50. Jaelani. 2007. Khasiat Bawang Merah. Kanisius, Yogyakarta, 13. James, J., Baker, C., and Swain, H. 2002. Prinsip-prinsip Sains untuk Keperawatan. Penerjemah: Wardhani, I. R. Judul buku asli: Principles of Science for Nurses. Penerbit Erlangga, Jakarta, 120. Karneli., Kaewiti, W., dan Rahmalia, G. 2014. Pengaruh Ekstrak Bawang Merah (Alluim ascalonicum L.) terhadap Pertumbuhan Staphylococcus sp. Misna., dan Diana, K. 2016. Aktivitas Antibakteri Ekstrak Kulit Bawang Merah (Allium cepa L.) terhadap Bakteri Staphylococcus aureus. Galenika Journal of Pharmacy 2 (2): 138-144. Morison, M.J. 2003. Manajemen Luka. Penerjemah : Tyasmono. Judul buku asli: A Colour Guide to The Nursing Management of Wounds. EGC, Jakarta, 2-3, 10 Praworo, K. 2011. Terapa Medipic: Medical Picture. Penebar Plus, Jakarta, 27. Rahayu, E., dan Berlian, N.V.A. 2004. Bawang Merah. Penebar Swadaya, Jakarta, 4-5. Rahmawati, R., dan Rosyadi, H. 2013. Povidone Iodine 10% (Betadine) dan Daun Sirih dalam Mempercepat Penyembuhan Luka Bersih Marmut. Journals of Ners Community 4 (1): 52-57. Redaksi Agro Media. 2008. Buku Pintar Tanaman Obat. PT Agro Media Pustaka, Jakarta, 21. Sajidah, A., Warnis W, G.S.P., dan Sugijati. 2014. Perbedaan Lama Penyembuhan Luka Bersih antara Perawatan Luka Menggunakan Gerusan Bawang Merah (Allium cepa L) dibandingkan dengan Povidon Iodine 10% pada Tikus Putih (Rattus Norvegicus Strain Wistar). Media Bina Ilmiah 8 (4): 37-43. Salasia, S. I. O., dan Hariono, B. 2016. Patologi Klinik Veteriner. Penerbit Samudra Biru, Yogyakarta, 1, 9. Samadi, B., dan Cahyono, B. 2005. Bawang Merah Intensifikasi Usaha Tani. Kanisius, Yogyakarta, 10-11. Santoso, H.B. 1998. Toga 2 Tanaman Obat Keluarga. Kanisius, Yogyakarta, 38-39. Satria, R. G. D. 2017. Konsep Dasar dan Cara Praktis Belajar Analisis Statistik dengan SPSS. PT. Global Byakta Waylaay, Yogyakarta, 42-46, 63-64. Sihombing, M., dan Tuminah S. 2011. Perubahan Nilai Hematologi, Biokimia Darah, Bobot Organ dan Bobot Badan Tikus Putih pada Umur Berbeda. Jurnal Veteriner 12 (1): 58-64. Sjamsuhidajat, R., dan Jong, D. 2010. Buku Ajar Ilmu Bedah (Edisi 3). ECG, Jakarta, 95-97 Smith, J. B., dan Mangkoewidjojo, S. 1988. Pemeliharaan, Pembiakan dan Penggunaan Hewan Percobaan di Daerah Tropis. UI Press, Jakarta, 40, 43. Sumardjo, D. 2006. Pengantar Kimia. EGC, Jakarta, 18. Suriadi. 2004. Perawatan Luka Edisi 1. Sagung Seto, Jakarta, 9-11. Tambayong, J. 1999. Patofisiologi untuk Keperawatan. EGC, Jakarta, 56. Utomo, J. S., Sukoco, R., Galih, A., dan Salasia, S. I. O. 2006. Peranan Buah Mengkudu (Morinda citrifolia) terhadap Aktivitas Fagositosis Leukosit Polimorfonuklear Tikus (Rattus novergicus) yang diberi Diet Lemak Tinggi. Jurnal Peternakan 3 (2): 23-28. Weihe, W. H. 1987. The Laboratory Rat. In The UFAW Handbook on The Care and Management of Laboratory Animals. Longman Scientific & Technical, London, 309-330. WHO (World Health Organization). 1995. Kader Kesehatan Masyarakat. Penerjemah: Heru, A. Judul buku asli: The Community Health Worker. EGC, Jakarta, 159.

DOI: https://doi.org/10.22146/jsv.44891