Deteksi Johne’s Disease pada Sapi Potong di Kabupaten Kebumen Berdasarkan Uji Elisa
Iip Zuliana Rachmawati(1*), Widagdo Sri Nugroho(2), Rahmat Setya Adji(3)
(1) Stasiun Karantina Pertanian Kelas I Biak Papua
(2) Fakultas Kedokteran Hewan Universitas Gadjah Mada, Yogyakarta
(3) Balai Besar Penelitian Veteriner, Bogor
(*) Corresponding Author
Abstract
Kebumen Regency have been set as the source of the beef cattle breeds by the Ministry of Agriculture of the Republic of Indonesia. As the parent stock of beef cattle, these should be free from any infectious disease. Base on Balai Besar Veterinary Wates survey at 2015, some beef cattle in some districts at Kebumen indicated Johne's Disease. This research was carried out with the epidemiologic approach to detect Johne's Disease. About 173 serum specimens were taken from 173 heads of cows who have clinical symptoms of Johne's Disease. Sera were analyzed by ELISA. Base on ELISA result, 38 from 173 serum were seropositive. These cows sample come from local ancestor and there were not Johne’s disease vaccination program in Kebumen. This research proved that Johne's Disease have occurred in this populations which dedicated for parent stock of beef cattle in the District of Kebumen. Disease control program for Johne’s disease should be undertaken in this area to prevent the disease transmition.
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Abdellrazeq, G.G; Elnaggar, M.M.; Khaliel, S.A.; Gamal-Eldin, A.E. 2014 Detection of Mycobacterium avium subsp. paratuberculosis from cattle and buffaloes in Egypt using traditional culture, serological and molecular based methods. www. veterinaryworld,org/Vol.7/August-2014/7.pdf.
Adji, R.S. 2008. Deteksi Mycobacterium avium subspesies paratuberculosis padasapi perah di Kabupaten Bandung dan Banyumas. [tesis]. Bogor (ID):Sekolah Pascasarjana, Institut Pertanian Bogor.
[BPS] Badan Pusat Statistik. 2016. Kebumen Dalam Angka tahun 2015.
Carvalho, I.A.; Campos, V.E.B.; Souza1, I.S.; Zanardo, L.G.; Filho, J.D.R; Gomes M.J.P.; Maria A. S. Moreira, M.A.S. 2012. Diagnosis Of Paratuberculosis In Cattle: Microbiological Culture, Serology And PCR. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3768850/pdf/bjm-43-581.pdf
Celik, A, Turutoglu, H. 2017. Seroprevalence of Paratuberculosis in Cattle, Sheep, and Gaots in Burdur, Southwestern Turkey. IJVM 72 (1): 30-36
Kurade, N.P.; Tripathi, B.N.; Rajukumar, K.; Parihar, N.S. 2004. Sequential Development of Histologic Lesions and Their Relationship with Bacterial Isolation, FecalShedding, and Immune Responses during Progressive Stages of ExperimentalInfection of Lambs with Mycobacterium avium subsp. paratuberculosis. VetPathol. 41:378-387
Martin, S.W.; A.H. Meek and P. Willeberg. 1987.Veterinary epidemiology principles and methods. Univ.Press, Iowa States. p 35.McKenna, S.L.B.D.C.; Socket, G..P.; Keefe, J.; Mcclure, J.A.; Vanleeuwen and H.W. Barkema. 2005 Comparison oftwo enzyme-linked immunosorbent assay for diagnosisof Mycobcaterium avium subsp.paratuberculosis. J. Vet.Diagn. Invest. 17: 426-466.
Nugroho, W.S. 2008. Deteksi Mycobacterium avium subspesies paratuberculosis pada sapi perah, susu pasteurisasi, dan susu formula lanjutan di wilayah Bogor. [disertasi]. Bogor (ID): Sekolah Pascasarjana, Institut Pertanian Bogor.
Osterstock, J.B.; Fosgate, G.T.; Norby, B.O.; Manning, E.J.B.; Collins M.T.; Roussell, A.J. 2007.Contribution of enveriontmental Mycobacteria to falsepositive serum ELISA results for Paratuberculosis, J.Am. Vet. Med. Assoc. 230: 896-901.
Riad, E.M.; Amany, N D.; Mohsen, D.M. 2016. Evaluation of ELISA and The Conventional Methods Used in JOHNE’ S Disease Diagnosis in Cows. Egypt. J. Chem. Environ. Health, 2 (2):324-334.
Roussel, A.J.; Fosgate, G.T.; Manning, E.J.B.; Collins, M.T 2007. Association of fecal shedding ofMycobcateria with ELISA-determined seroprevalence
for Paratubeculosis in beef herds. J. Am. Vet. Med.Assoc. 230: 890-895.
Tagueha, A.D.; Nugroho, W.S. 2012. Paratuberculosis. dalam Widiasih, D.A., Budiharta S. (eds) : Epidemiologi Zoonosis di Indonesia. UGM Press : Yogyakarta. Halaman 164-.182.
Wolf, R.; Orsel, K; De Buck, J.; Barkema, H.W. 2015. Calves shedding Mycobacterium avium subspecies paratuberculosis are common on infected dairy farms. Veterinary Research (2015) 46:71DOI: https://doi.org/10.22146/jsv.41154
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