* Corresponding Author
Respons Imun Ayam Petelur Pascavaksinasi Newcastle Disease dan Egg Drop Syndrome
https://doi.org/10.22146/jsv.29295 Gusti Ayu Kencana(1*), I Nyoman Suartha(2), Daniel Raja Bonar Nainggolan(3), Agatha Seren Lumban Tobing(4)
(1) Fakultas Kedokteran Hewan, Universitas Udayana
(2) Fakultas Kedokteran Hewan, Universitas Udayana
(3) Fakultas Kedokteran Hewan, Universitas Udayana
(4) Fakultas Kedokteran Hewan, Universitas Udayana
(*) Corresponding Author
Abstract
Some viral diseases in poultry could lead to huge losses to the farmers. Newcastle Disease (ND) and Egg Drop Syndrome (EDS) are a group of infectious viral disease that can cause the decreasein egg production. Newcastle Disease is caused by Avian paramyxovirus type 1 (PMV-1) Paramyxoviridae family. The causative agent of EDS is Duck adenovirus-I Adenoviridae family. Both of these diseases affect the economic losses to the poultry. The main action to prevent hens from ND and EDS virus diseases is vaccination. The success of
vaccination can be tested by serology. ND and EDS virus characteristically agglutinate hen’s erythrocyte they have Hemagglutine protein on virus envelope so can be tested by hemagglutination. The study was conducted on
a commercial poultry farm in order to determine the success of vaccination against ND and EDS. The hens were vaccinated by Newcastle Disease-Infectious Bronchitis- Egg Drop Syndrome (ND-IB-EDS) inactivated vaccines.
Serological test was conducted in pre and post vaccination by using microtiter hemagglutination test. The antibody titre is expressed in units of HI log2. The results of the study, the mean antibody titer against ND pre vaccination
was 4,53 ± 1,356 HI log2 and antibody titre in 2nd, 3rd and 4th week were 8,67 ± 0,617 HI log2, 7,73 ± 1,335 HI log2 and 5,20 ± 0,862 HI log2 post vaccination. Antibody titre against EDS pre vaccination was 0 ± 0,000 HI log2 and antibody titre post vaccination in 2nd, 3rd and 4th week were 7 ± 1,363 HI log2, 7,27 ± 1,438 HI log2 and 7,6 ± 1,056 HI log2. It showed that ND-IB-EDS inactivated vaccines is serological protective for ND and EDS titres.
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(ACFAF) ASEAN Cooperation in Food, Agriculture
and Foresty. 2012. ASEAN Standards for
Animal Vaccines, Second Edition. Livestock
Publication Series. http:/www.asean.org/
communities/asean-economiccommunity/
categ ory/publication-3. (2 April 2016).
Aiyer-Harini P., Ashok-Kumar H.G., Kumar
GP.,Shivakumar N. 2013. An Overview
ofImmunologic Adjuvants-A Review. J
Vaccines Vaccine 4(1): 1-4.
Aldous EW, Mynn JK, Banks J, Alexander DJ (2003).
A molecular epidemiological study of avian
paramyxovirus type 1 (Newcastle disease
virus) isolates by phylogenetic analysis of
a partial nucleotide sequence of the fusion
protein gene. J. Avian Pathol. 32, 239-256.
Alexander D.J. and Senne D.A. 2008b. Newcastle
Disease and Other Avian Paramyxoviruses.
In: A Laboratory Manual for the Isolation,
Identification and Characterization of Avian
Pathogens, Dufour-Zavala L. (Editor in
Chief) Swayne D.E., Glisson J.R., JackwoodM.W., Pearson J.E., Reed W.M, Woolcock P.R., 4th ed., American Association of Avian
Pathologists, Athens, GA, 135–141.
Al-Habeeb M.A., Mohamed M.H.A., Sharawi
S. 2013. Detection and characterization of
Newcastle disease virus in clinical samples
using real time RT-PCR and melting curve
analysis based on matrix and fusion genes
amplification. Veterinary World 6(5):239-243.
Ashraf, A and Shah, M. S. 2014. Newcastle
Disease: Present status and future
challenges for developing countries.
African Journal of Microbiology Research
(5): 411-416.
Badar, S.T., Siddique, M., Ali, R., dan Rasool,
M.H. 2006. Serological Status of Egg Drop
Syndrome in Breeders and Commercial Layers
in Manshera District. Pakistan vet. J. 26(1):33-35.
Begum, J.A., Chowdhury, E.H., Parvin, R., Matin,
M.A., Giasuddin, M., Bari, A.S.M., Islam, M.R.
Detection of Egg Drop Syndrome Virus
by Polymerase Chain Reaction. International
Journal of Livestock Research3(2): 112-116.
Bidin, Z., Lojkic, I., Mikec, M., Pokric, B. 2007.
Naturally Occurring Egg Drop Syndrome
Infection in Turkeys. ACTA VET BRNO 76:415-421.
Cattoli, G., Susta, L., Terregino, C., Brown, C.
Newcastle disease: a review of field
recognition and current methods of laboratory
detection. JVDI 23(4): 637-656
Cardoso, W.M., Aguiar, F.J.L.C., Romão, J.M.,
Oliveira, W.F., Salles, R.P.R., Teixeira, R.S.C.,
Sobral, M.H.R. 2005. Effect of Associated
Vaccines on the Interference between
Newcastle Disease Virus and Infectious
Bronchitis Virus in Broilers. Brazilian J of
Poultry Sci 7(3): 181-184.
(CFSPH) The Center for Food Security and Public
Health. 2006. Egg Drop Syndrome. www.
cfsph.iastate.edu.(18 Oktober 2015).
Cho S.H., Kwon H.J., Kim T.E., Kim J.H., Yoo H.S.,
Park M.H., Park Y.H. & Kim S.J. (2008).
Characterization of a recombinant Newcastle
disease virus vaccine strain. Clin. Vaccine
Immunol., 15 (10), 1572–1579.
Ditjennak. 2014. Manual Penyakit Unggas.
Direktorat Kesehatan Hewan Direktorat
Jenderal Peternakan dan Kesehatan Hewan
Kementerian Pertanian. Hal 36.
Hafez M. 2011. Avian Adenoviruses Infections with
Special Attention to Inclusion Body Hepatitis/
Hydropericardium Syndrome and Egg Drop
Syndrome. Pak Vet J 31(2): 85-92.
Hewajuli, D.A dan Dharmayanti, N.L.P.I. 2015.
Peran Sistem Kekebalan Non-spesifik dan
Spesifik pada Unggas terhadap Newcastle
Disease. Wartazoa Vol. 25(3): 135-146.
Indriani R, Dharmayanti INLP. 2013. Studi Efikasi
Vaksin Bivalen AI Isolat Lokal terhadap
Beberapa Karakter Genetik Virus AI subtipe
H5N1. Jurnal Biologi Indonesia 9(1): 21-30.
Kencana, GA.Y., Suartha, IN., Simbolon, MP.,
Handayani, AN., Ong, S., Syamsidar.,
Kusumastuti, A. 2015. Respon Antibodi
Terhadap Penyakit Tetelo pada Ayam yang
Divaksin Tetelo dan Tetelo-Flu Burung.Jurnal
Veteriner Vol 16 (2): 283-290.
Kencana, GAY., Suartha, N., Paramita, NMAS.,
Handayani, AN. 2016. Vaksin Kombinasi
Newcastle Disease dengan Avian Influenza
Memicu Imunitas Protektif Pada Ayam Petelur
Terhadap Penyakit Tetelo dan Flu Burung.
Jurnal Veteriner Vol 17(2): 257-264.
Mayahi, M., Talazadeh, F., Aslahi, H. 2013. Effect
of the Commercial Mixed Live Newcastle
Disease and Infectious Bronchitis Vaccines
and the Use of Two Separate Vaccines
Given Simultaneously on Systemic
Antibody Responses. Iranian Journal of
Virology. 7(3): 17-21
Moomivand, H., Bassami, M.R., Faramarzi, S.,
Stabraghil, E., Ghaedi, A., Ghabel, H.,
Zarghami, A., Banaei, M. 2013. Serological
and Clinical Survey of Newcastle Disease in Broiler Chickens of East Azarbayjan by HITests. Euro J Exp Bio 3(6):311-314.
OIE (2012). Newcastle disease. Manual of
Diagnostic Tests and Vaccines for Terrestrial
Animals. Chapter 2.3.14. http://www.oie.
int/ international-standard-setting/terrestrialmanual/
access-online.
Orsi MA., Doretto Jr, L, Camillo SCA., Reischak
D., Ribeiro S.A.M., Ramazzoti A., Mendonça
A.O., Spilki FR., Buzinaro M.G., Ferreira
HL., Arns CW. 2010. Prevalence of Newcastle
Disease virus in Broiler chickens (Gallus
gallus) in Brazil. Brazilian J. Microbiol.41:349-357.
Salihu, A.E., Joannis, T.M., Onwuliri, F.C., Ibu,
J.O., Masdooq, A.A., Muazu, A., Haruna, G.,
Ngbede, J. 2010. Serological Evidence of
Egg Drop Syndrome’1976 (EDS’76) in Freerange
Chickens at Chicken Market Sites in Jos,
Nigeria. Turk. J. Vet. Anim. Sci 34(4): 403-406.
Saliu, O.J., Sanda, M.E., Audu, S.I. 2009. Adoption
of Vaccination Against Newcastle Disease by
Rural Poultry Women Farmers in the North
Central Zone of Nigeria. Int. J. Poult. Sci 8
(5): 500-503.
Suresh, P., Shoba, K., Rajeswar, J.J. 2013. Incidence
of egg drop syndrome – 1976 in Namakkal
district, Tamil Nadu, India. Vet. World
(6):350-353.
Wibowo, M.H., Amanu, S. 2010. Perbandingan
Beberapa Program Vaksinasi Penyakit
Newcastle Disease pada Ayam Buras.J. Sain
Vet. Fakultas Kedokteran Hewan. Universitas
Gadjah Mada. Yogyakarta. 28 (1): 1-9.
World Organization for Animal Health. 2009.
Newcastle Disease: Aetiology, Diagnosis,
Prevention, and Control References, OIE
Technical Disease Cards. OIE Scientific and
Technical Department, Thailand.
DOI: https://doi.org/10.22146/jsv.29295
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