PERBANDINGAN DAYA ANTIBAKTERI DISINFEKTAN INSTRUMEN PREPARASI SALURAN AKAR NATRIUM HIPOKLORIT 5,25%, GLUTARALDEHID 2%, DAN DISINFEKTAN BERBAHAN DASAR GLUTARALDEHID TERHADAP Bacillus subtilis

Abstract

Aseptic is a principle in dentistry that must be executed in daily practice by cross-infection control such as disinfection of endodontic instruments with high-level disinfectant (HLD). Some types of recognized high-level disinfectants are glutaraldehyde, glutaraldehyde-based disinfectant and sodium hypochlorite. The aim of this study is to compare the antibacterial potential of 5.25%
sodium hypochlorite, 2% glutaraldehyde, and glutaraldehyde-based disinfectant on B. subtilis.
Agar diffusion method was used in this study with 9 plate of Mueller-Hinton agar which was inoculated with B. subtilis culture McFarland scale 3. Three wells were made in each plate and each of the well was filled with a high-level disinfectant, which were 5.25% sodium hypochlorite, 2% glutaraldehyde, and glutaraldehyde-based disinfectant, then incubated and observed and measured its zone of inhibition. Data were analyzed with one-way ANOVA and post-hoc LSD test.
One-way ANOVA result showed that 5,25% sodium hypochlorite, 2% glutaraldehyde, and glutaraldehyde-based disinfectant were influential as root canal preparation instruments disinfectants against B. subtilis. LSD test result showed that there were significant differences between 5.25% sodium hypochlorite and 2% glutaraldehyde, 5.25% sodium hypochlorite with glutaraldehydebased
disinfectant; between glutaraldehyde 2% with glutaraldehyde-based disinfectant.
The study concluded that 2% glutaraldehyde  had the strongest antibacterial potential against B. subtilis, followed by  glutaraldehyde-based disinfectant, and the weakest is 5.25% sodium hypochlorite.