![Cover Image](https://jurnal.ugm.ac.id/public/journals/5/cover_article_95898_en_US.jpg)
Effects of explants and culture medium compositions on quality of chrysanthemum ‘Jayanti Agrihorti’ rooted cuttings
![](/public/site/images/admin/icons/icon-doi.png)
Herni Shintiavira(1), Dewi Pramanik(2), Ratna Dewi Daniyanti(3), Miranti Dian Pertiwi(4), Fitri Rachmawati(5*)
(1) Research Center for Horticulture, Research Organization for Agriculture and Food, National Research and Innovatioan Agency Republic of Indonesia (BRIN)
(2) Research Center for Horticulture, Research Organization for Agriculture and Food, National Research and Innovatioan Agency Republic of Indonesia (BRIN)
(3) Research Center for Horticulture, Research Organization for Agriculture and Food, National Research and Innovatioan Agency Republic of Indonesia (BRIN)
(4) Reseach Center for Sustainable Production System and Life Cycle Assesment, National Research and Innovation Agency Republic of Indonesia (BRIN)
(5) Research Center for Horticulture, Research Organization for Agriculture and Food, National Research and Innovatioan Agency Republic of Indonesia (BRIN)
(*) Corresponding Author
Abstract
'Jayanti Agrihorti' is a superior chrysanthemum therefore rooted cuttings quality is required. In vitro propagation increases the rate of propagation and produces true-to-type plants. The research to obtain the best explants and culture medium composition that is capable of producing high-quality plants. The research was arranged in a randomized complete block design consisting of two factors. The first factor was the explant, including the apical shoot of 0.5 cm, 1.0 cm, and the nodal segment. The second factor was in vitro culture medium composition (CP), consisting of CP1 (Murashige and Skoog (MS) medium for initiation, followed by MS + 2.5 mg.L⁻¹ gibberellic acid (GA₃) for subculture), CP2 (MS + 0.25 mg.L⁻¹ benzyl amino purine (BAP) for initiation, followed by MS for subculture), CP3 (MS + 0.25 mg.L⁻¹ BAP for initiation, followed by MS + 2.5 mg.L⁻¹ GA₃ for subculture), and CP4 (MS + 0.5 mg.L⁻¹ BAP for initiation, followed by MS + 0.25 mg.L⁻¹ BAP for subculture). Acclimatization was performed after third subculture. The apical shoot size of 0.5−1.0 cm is optimum for producing chrysanthemum-rooted cuttings. Meanwhile, MS medium for initiation stage, followed by MS + 2.5 mg.L⁻¹ GA₃ for subculture is the best culture medium composition for in vitro propagation. This explant and culture medium composition produced higher chlorophyll a, b, and a+b content, thereby resulting in higher plant, more leaves, larger stem diameter, and longer root length. This are recommended for chrysanthemum propagation, particularly in 'Jayanti Agrihorti'.
Keywords
Full Text:
PDFReferences
Arumugam, G., Sinniah, U. R., Swamy, M. K., and Lynch, P. T. (2020). Micropropagation and essential oil characterization of Plectranthus amboinicus (Lour.) Sprengel, an aromatic medicinal plant. Plant Tissue Culture, 56, pp. 491–503.
Brailko, V. A., Mitrofanova, O. V., Smykova, N. V., and Mitrofanova, I. V. (2018). Some morphological and physiological features of chrysanthemum under in vitro culture. Acta Horticulturae, 1201, pp. 607–612.
Dierck, R., Leus, L., Dhooghe, E., Van Huylenbroeck, J., De Riek, J., Van Der Straeten, D., and De Keyser, E. (2018). Branching gene expression during chrysanthemum axillary bud outgrowth regulated by strigolactone and auxin transport. Plant Growth Regulation, 86(1), pp. 23–36.
Eisa, E. A., Tilly-Mandy, A., Honfi, P., Shala, A. Y., and Gururani, M. A. (2022). Chrysanthemum : A comprehensive review on recent developments on in vitro regeneration. Biology, 11(1774), pp. 1–27.
Imtiaz, M., Khattak, A. M., Khan, M. A., Jalal, F., Hussain, S., Said, F., and Bo, H. (2019). Rapid in-vitro propagation of chrysanthemum morifolium through shoot bud explants. Pakistan Journal of Botany, 51(3), pp. 1093–1098.
International, F. (2022). Perfectly customised Chrysants. February, pp. 16–19.
Jahan, M. T., Islam, M. R., Islam, S. A. M. S., Das, P., Islam, M. M., Kabir, M. H., and Mamun, A. N. K. (2021). Clonal propagation of Chrysanthemum morifolium ramat using various explants obtained from field grown plants. GSC Biological and Pharmaceutical Sciences, 16(2), pp. 087–093.
Jevremovic, S., Subotic, A., Miljkovic, D., Trifunovic, M., Petric, M., and Cingel, A. (2012). Clonal fidelity of Chrysanthemum cultivars after long term micropropagation by stem segment culture. Acta Hort., 961, pp. 212–216.
Khaleghi, E., Arzani, K., Moallemi, N., and Barzegar, M. (2013). Evaluation of chlorophyll content and chlorophyll fluorescence parameters and relationships between chlorophyll a, b and chlorophyll content index under water stress in Olea europaea cv. Dezful. World Academy of Science Engineering and Technology, 68, pp. 1154–1157.
Kulus, D., Dieu, J. De, and Barkin, M. (2020). Growth Regulation and Validation of Homogeneity in In Vitro Derived Bleeding Heart by Molecular Markers and Spectral Analysis of Pigments. Journal of Plant Growth Regulation, 40, pp. 1521–1538.
Laplaze, L., Benkova, E., Casimiro, I., Maes, L., Vanneste, S., Swarup, R., Weijers, D., Calvo, V., Parizot, B., Herrera-Rodriguez, M. B., Offringa, R., Graham, N., Doumas, P., Friml, J., Bogusz, D., Beeckman, T., and Bennett, M. (2007). Cytokinins act directly on lateral root founder cells to inhibit root initiation. Plant Cell, 19(12), pp. 3889–3900.
Lazare, S., Bechar, D., Garbowicz, K., Fernie, A. R., Brotman, Y., and Zaccai, M. (2021). When vegetation indicates reproduction: The affinity between leaf morphology and flowering commitment in the lily meristem. Physiologia Plantarum, 172(4), pp. 2022–2033.
Meziani, R., Mazri, M. A., Arhazzal, M., Belkoura, I., Alem, C., and Jaiti, F. (2019). Evaluation of in vitro shoot elongation and rooting of date palm, and determination of physiological characteristics of regenerated plantlets. Not. Sci. Biol., 11(1), pp. 77–85.
Miao, H., Li, C., Duan, Y., Wei, L., Ju, M., and Zhang, H. (2020). Identification of a Sidwf1 gene controlling short internode length trait in the sesame dwarf mutant dw607. Theor. Appl. Genet., 133(1), pp. 73–86.
Mihovilović, A. B., Jerčić, I. H., Prebeg, T., Tomaz, I., Pavičić, A., Barić, M., and Kereša, S. (2020). Light source and cytokinin type affect multiplication rate, chlorophyll content and stomata formation of amelanchier alnifolia shoots in vitro. Journal of Central European Agriculture, 21(4), pp. 826–838.
Miler, N., and Zalewska, M. (2014). Somaclonal variation of chrysanthemum propagated in vitro from different explants types. Acta Scientiarum Polonorum, 13(2), pp. 69–82.
Pant, M., Lal, A., and Jain, R. (2015). A simple cost effective method for mass propagation of Chrysanthemum morifolium and antibacterial activity assessment of in vitro raised plantlets. J. Appl. Pharm.Sci., 5(07), pp. 103–111.
Polanco, M. C., and Ruiz, M. L. (1997). Effect of benzylaminopurine on in vitro and in vivo root development in lentil, Lens culinaris Medik. Plant Cell Reports, 17, pp. 22–26.
Sanjaya, L. L. (2021). Krisan Varietas Jayanti Agrihorti. In: 700 Teknologi Inovatif + 10 Model Penerapan Inovasi Kolaboratif. Jakarta: IAARD Press, pp. 295.
Shintiavira, H., Rahmawati, I., and Winarto, B. (2014). Application of modified generic media on in vitro culture production of qualified chrusanthemum seed (Dendranthema grandiflora Tzvelev). J. Hort, 24(3), pp. 220–229.
Tymoszuk, A., and Miler, N. (2019). Silver and gold nanoparticles impact on in vitro adventitious organogenesis in chrysanthemum, gerbera and Cape Primrose. Scientia Horticulturae, 257(April), 108766.
Ubeda-Tomas, S., Federici, F., Casimiro, I., Beemster, G. T. S., Bhalerao, R., Swarup, R., Doerner, P., Haseloff, J., and Bennett, M. J. (2020). Report gibberellin signaling in the endodermis controls Arabidopsis root meristem size. Curr. Biol., 19(July 28), pp. 1194–1199.
Wang, R. R., Gao, X.X., Chen, L., and Wang, Q.-C. (2014). Cryopreservation of Dendrathema morifolium “Japanese Red” shoot tips by droplet-vitrification. Acta Horticulturae, 1039, pp. 187–192.
Waseem, K., Jilani, M. S., and Khan, M. S. (2009). Rapid plant regeneration of chrysanthemum (Chrysanthemum morifolium l.) through shoot tip culture. African Journal of Biotechnology, 8(9), pp. 1871–1877.
Winarto, B. (2017). Teknologi Produksi Benih Berkualitas Pada Krisan Menggunakan Tunas Pucuk. Iptek Hortikultura, July, pp. 11–18.
Wu, Y., Gong, W., and Yang, W. (2017). Shade inhibits leaf size by controlling cell proliferation and enlargement in soybean. Scientific Reports, 7(1), pp. 1–10.
Yesmin, S., Hashem, A., Das, K. C., Hasan, M. M., and Islam, M. S. (2014). Efficient In Vitro Regeneration of Chrysanthemum (Chrysanthemum morifolium Ramat.) Through Nodal Explant Culture. Nuclear Science and Applications, 23(1&2), pp. 47–50.
Zalewska, M., Miler, N., and Wenda-Piesik, A. (2010). Effect of in vitro topophysis on the growth, development, and rooting of chrysanthemum expiants (Chrysanthemum X grandiflorum /Ramat/ Kitam). Journal of Horticultural Science and Biotechnology, 85(4), pp. 362–366.
Zhang, Z., Lee, Y., Spetz, C., Clarke, J. L., Wang, Q., and Blystad, D.-R. (2015). Invasion of shoot apical meristems by Chrysanthemum stunt viroid differs among Argyranthemum cultivars. Frontiers in Plant Science, 6(February), pp. 1–10.
![](/public/site/images/admin/icons/icon-doi.png)
Article Metrics
![](/public/site/images/admin/icons/icon-graph.png)
![](/public/site/images/admin/icons/icon-pdf.png)
Refbacks
- There are currently no refbacks.
Ilmu Pertanian (Agricultural Science) ISSN 0126-4214 (print), ISSN 2527-7162 (online) is published by Faculty of Agriculture Universitas Gadjah Mada collaboration with Perhimpunan Sarjana Pertanian Indonesia (PISPI) and licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.