Effects of auxin and cytokinin levels on the success of air layering in tea plant clones of GMB 7 and GMB 9 using husk charcoal, cocopeat and moss media
Ika Betty Widyastuti(1*), Prapto Yudono(2), Eka Tarwaca Susila Putra(3)
(1) Directorate of Estate Crops Seeding, Directorate General of Estate Crops, Ministry of Agriculture Jln. Harsono RM no.3, Gedung C Lantai 4, Ragunan, Jakarta Selatan 12550
(2) Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora no. 1, Bulaksumur, Sleman, Yogyakarta 55281
(3) Department of Agronomy, Faculty of Agriculture, Universitas Gadjah Mada Jln. Flora no. 1, Bulaksumur, Sleman, Yogyakarta 55281
(*) Corresponding Author
Abstract
The research aimed to propagate tea plants by air layering in order to obtain new plants with shorter immature plants period (TBM), which is 1.5 years, by utilizing wasted branches from routine clean pruning activities in tea plantations. The research was conducted from August 2018 to January 2019 at tea plantation of PT. Pagilaran, Batang, Central Java. Experiment using single factor treatment was arranged in a Completely Randomized Design with three replications. The treatment was layering media, consisting of husk charcoal, moss, and cocopeat, which were applied on GMB 7 and GMB 9 clones. The results showed significant effects of the layering media on the levels of auxin and cytokinin in GMB 7. The highest levels were found in the husk charcoal and moss media. Analysis of sucrose, glucose and total sugar as well as the physiological analysis of the air layering roots showed no significant difference in the fresh weight, dry weight, volume, surface area, diameter, and length of the roots. Husk charcoal resulted in the highest success rate of the air layering in GMB 7, which was 100%. Meanwhile, GMB 9 showed significant difference only in the auxin levels. There was no significant effect of layering media on the analysis of sucrose, glucose and total sugar as well as on the physiological analysis of the air layering roots. Both husk charcoal and moss media resulted in the highest success rate of the air layering in GMB 9, which was 58.33%.
Keywords
Full Text:
PDFReferences
Adinugraha, H.A.S, Pujiono, and T. Herawan. 2007. Teknik perbanyakan vegetatif jenis tanaman Acacia mangium. Info Teknis, 5 :1-6.
Adisewojo, S. 1964. Bertjotjok tanam teh. 3th ed., Bandung: Sumur publisher.
Angelina, N., L. Siregar, and L.A. Putri. 2017. Pengaruh zat pengatur tumbuh terhadap induksi akar (Rhizogenesis) pada tanaman bangun-bangun (Plectranthus amboinicus (Lour.) Spreng) secara in vitro. J Agroekoteknologi AF USU., 82: 644-649.
Arifin, S. 2014. Gerakan penyelamatan agribisnis teh nasional (GPATN). 2th ed., Jakarta: Indonesian Tea Board.
Hartmann, H.T.; D.E. Kester; F.T. Davies Jr. and R.L. Geneve. 1997. Plant propagation : Principles and practice. 6th ed., USA: Prentice-Hall, Upper Saddle River, NJ.
Hasriani, D.K. Kalsim and A. Sukendro. 2012. Kajian serbuk sabut kelapa (cocopeat) sebagai media tanam. 1st ed., Bogor: IPB.
Istomo and N. Valentino. 2012. Pengaruh perlakuan kombinasi media terhadap pertumbuhan anakan tumih (Combretocarpus rotundatus (Miq.) Danser). Jurnal Silvikultur Tropika, 3: 81-84.
Lestari, E.G. 2011. Peranan zat pengatur tumbuh dalam perbanyakan tanaman melalui kultur jaringan. Jurnal Agrobiogen, 1: 63–68.
Ministry of Agriculture. 2014. Development of Indonesian tea market in domestic market and internasional market in 2014. 1st ed. Jakarta: Balai Penelitian Tanaman Industri dan Penyegar Kementerian Pertanian.
Miyashita, T., O. Takafumi, S. Fukashi, A. Hajime and H. Yoichiro. 2009. Plant regeneration with maintenance of the endosperm ploidy level by endosperm culture in lonicera caerulea var. emphyllocalyx. Plant Cell, Tissue, & Organ Culture, 3: 291-301.
Prastowo, N. dan J.M. Roshetko. 2006. Teknik pembibitan dan perbanyakan vegetatif tanaman buah. 1st ed. Bogor: World Agroforestry Center.
Rochiman, K. dan S.S. Harjadi. 1974. Pembiakan Vegetatif Pengantar Agronomi. 1st ed. Bogor : IPB Press.
Samudin, S. 2009. Pengaruh kombinasi auksin – sitokinin terhadap pertumbuhan buah naga. Media of Central Sulawesi Research Center,1: 62–66.
Schaller, G.E., A. Bishopp, J. Joseph and Kieberc. 2015. The Yin-Yang of hormones : Cytokinin and auxin interactions in plant development. The Plant Cell, 27: 44–63.
Shofiyani, A and M. Purnawanto. 2017. Pertumbuhan kalus kencur (Kaemferia galanga L.) pada komposisis media dengan perlakuan sukrosa dan zat pengatur tumbuh (2,4 D dan benzyl aminopurin). Agritech, 19: 55 – 64.
Widiastoety, D and Nurmalinda. 2010. Pengaruh suplemen nonsintetik terhadap pertumbuhan plantlet anggrek vanda. Jurnal Hortikultura, 20: 60-66
Widyastuti, N and T. Donowati. 2007. Peranan beberapa zat pengatur tumbuh (ZPT) tanaman pada kultur in vitro. Jurnal Sains dan Teknologi Indonesia, 3: 55-63.
Woodward, W. Andrew and B. Bartel. 2005. Auxin : Regulation, action, and interaction. Annals of Botany, 95: 707–735.
DOI: https://doi.org/10.22146/ipas.53019
Article Metrics
Abstract views : 2359 | views : 2028Refbacks
- There are currently no refbacks.
Ilmu Pertanian (Agricultural Science) ISSN 0126-4214 (print), ISSN 2527-7162 (online) is published by Faculty of Agriculture Universitas Gadjah Mada collaboration with Perhimpunan Sarjana Pertanian Indonesia (PISPI) and licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.