* Corresponding Author
The Effect of Priming Duration with Salicylic Acid under Salinity Stress on Growth and Leaf Anatomy of Sweet Corn (Zea mays L.)
https://doi.org/10.22146/ipas.39621 Krisnanda Surya Dharma(1), Maryani Maryani(2*)
(1) Faculty of Biology, Universitas Gadjah Mada Jl. Teknika Selatan,Sekip Utara, Yogyakarta 55283
(2) Faculty of Biology, Universitas Gadjah Mada, Yogyakarta Jl. Teknika Selatan,Sekip Utara, Yogyakarta 55283
(*) Corresponding Author
Abstract
Salinity stress is known for adverse effect on plants. Priming with salicylic acid was able to improve plant performance under salinity stress. This study aimed to determine the effect of priming duration with salicylic acid on growth, leaf anatomy and the optimal priming duration for sweet corn seedlings (Zea mays L.) under salinity stress. The experiment was based on Completely Randomized Design with two factors and five replications. The first factor was priming duration with salicylic acid (2 mM) with four different durations (0, 12, 18 and 24 h). The second factor was the level of salinity (NaCl 0% and 3%). Parameters observed were germination percentage, plant height, root length, fresh weight, dry weight, chlorophyll content, leaf proline content, leaf anatomy and stomatal density. Data were analyzed with t-test, ANOVA and followed by Duncan’s test at 95% confidence level. The results showed that 18-h priming duration observed as the highest germination percentage which was 7% higher than control. Priming for 24 h showed phytotoxic effect for sweet corn on the germination phase by reducing the percentage of germination. The application of salicylic acid mitigated the toxic effects of NaCl stress on maize seedlings and considerably improved root and shoot growth, photosynthetic pigments, fresh weight, dry weight, proline content, and stomatal density, as well as could maintaining the leaf anatomy. The optimal priming duration with salicylic acid for sweet corn seedlings under 3 % salinity was 18 h.
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Adillah, A. 2016. Pertumbuhan dan Produksi Beberapa Aksesi Hotong (Setaria italica (L.) Beauv.) pada Cekaman Salinitas. Skripsi. IPB. Bogor. pp. 5-6. Available at http://repository.ipb.ac.id/jspui/bitstream/123456789/79559/1/A16aad.pdf
Agami, R.A. 2013. Alleviating the adverse effects of NaCl stress in maize seedlings by pretreating seeds with salicylic acid and 24-epibrassinolide. South African Journal of Botany 88, pp. 171–177. Available at https://www.sciencedirect.com/science/article/pii/S0254629913003220
Anthraper, A., Dubois, J.D. 2003. The effect of NaCl on growth, N2 fixation and percentage total nitrogen in Leucaena leucacephala var K-8. American Journal of Botany, 90:683-692. Available at https://www.ncbi.nlm.nih.gov/pubmed/21659163
Arfan, M., Athar, H.R., Ashraf, M. 2007. Does exogenous application of salicylic acid through the rooting medium modulate growth and photosynthetic capacity in two differently adapted spring wheat cultivars under salt stress? Journal of Plant Physiology, 164, pp.685-694. Available at https://www.ncbi.nlm.nih.gov/pubmed/16884826
Arif, M., Jan, M.T., Khan, N.U., Khan, A., Khan, M.J., Munir, I. 2010. Effect of seed priming on growth parameters of soybean. Pakistan Journal of Botany, 42(2), pp. 2803-2812.
Bastias, E., Gonzales-Moro, M.B., Gonzales-Munia, C. 2004. Zea mays L. amylacea from the Lluta Valley (Africa-Chile) tolerates salinity stress when high levels of boron are available. Plant and Soil, 267, pp. 73-84. Available at https://link.springer.com/content/pdf/10.1007/s11104-005-4292-y.pdf
Bates, L.S., Waldren, R.P., Tare, I.D. 1973. Rapid determination of free proline for water-stress studies. Plant, Soil, 39, pp. 205–207. Available at https://link.springer.com/article/10.1007/BF00018060
Batool N., Shahzad A., Ilyas N. and Noor T. 2014. Plant and salt stress. International Journal of Agriculture Crop Science, 7 (9), pp. 582-589. Available at ijagcs.com/wp-content/uploads/2014/06/582-589.pd
Boudsocq, M., Laurière, C. 2005. Osmotic Signaling in Plants. Multiple Pathways Mediated by Emerging Kinase Families . Plant Physiology, 138 (3), pp. 1185-1194. Available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1176393/
Carcamo, H.J., Bustos, R.M., Fernandez, F.E., Bastias, E.I. 2012. Mitigating effect of salicylic acid in the anatomy of the leaf of Zea mays L. Iluteno ecotype from the Lluta Valley (Arica-Chile) under NaCl. Idlesia, 30(3), pp. 55-63. Available at www.scielo.cl/pdf/idesia/v30n3/art07.pdf
Dachlan, A., Kasim, N., Sari, A.K. 2013. Uji ketahanan salinitas beberapa varietas jagung (Zea mays L.) dengan menggunakan agen seleksi NaCl. Biogenesis, 1(1), pp. 9-17. Available at journal.uin-alauddin.ac.id/index.php/biogenesis/article/view/442
Djukri. 2009. Cekaman Salinitas terhadap Pertumbuhan Tanaman. Prosiding Seminar Nasional Penelitian, Pendidikan dan Penerapan MIPA, Fakultas MIPA, Universitas Negeri Yogyakarta, Yogyakarta. pp. 49-55. Available at eprints.uny.ac.id/12120/1/Bio_Djukri1%2C%20UNY.pdf
Farahmandfar, E., Shirvan, M.B., Sooran, S.A., Hoseinzadeh, B. 2013. Effect of seed priming on morphological and physiological paramaters of fenugreek seedlings under salt stress. IJACS Journal, 5(8), pp. 811-815. Available at https://www.researchgate.net/.../292258491_Effect_of_seed_priming_on_morphologica
Fariduddin, Q., Hayat, S., Ahmad, A. 2003. Salicylic acid influences net photosynthetic rate, carboxylation efficiency, nitrate reductase activity, and seed yield in Brassica juncea. Photosynthetica, 41, pp. 281-283. Available at https://link.springer.com/article/10.1023/B:PHOT.0000011962.05991.6c
GTAC, 2016. Measuring Stomatal Density (Leaf Impression Method). Gene Technology Access Centre. Victoria. pp.1-4. Available at https://www.gtac.edu.au/wp-content/uploads/.../StomatalDensity_LabPreparation.pdf
Gunes, A., Inal A., Alpaslan, M., Eraslan, F., Guneri, E., Cicek, N. 2005. Salicylic acid changes on some physiological parameters symptomatic for oxidative stress and mineral nutrition in maize (Zea mays L.) grown under salinity. Journal of Plant Physiology, 164, pp. 728-736. Available at https://www.sciencedirect.com/science/article/pii/S0176161706000496
Harborne, J.B. 1998. Phytochemical Methods: A Guide to Modern Techniques of Plant Analysis 3rd Edition. Chapman & Hall. London. pp. 227-229.
Herlina, N.F.N. & Aziz, S.A. 2016. Peningkatan viabilitas benih jintan hitam (Nigella sativa) dengan hidropriming dan pemberian asam giberelat. Buletin Penelitian Tanaman Rempah dan Obat, 27(2), pp. 129-136. Available at ejurnal.litbang.pertanian.go.id/index.php/bultro/article/view/3920
Hernandez, J.A., Ferrer, M.A., Jimenez, A., Ros-Barcelo A., Sevilla, F. 2001. Antioxidant system and O2-/H2O2 production in the apoplast of Pisum sativum L. leaves: its relation with NaCl-induced necrotic lesions in minor veins. Plant Physiology, 127, pp. 817-834. Available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC129254
Imran, M. Nutrient Seed Priming Improves Abiotic Stress Tolerance in Zea mays L. and Glycine max L. 2012. (Dissertation). Institute of Crop Science, Nutritional Crop Physiology, University of Hohenheim. pp.46-55. Available at https://opus.uni-hohenheim.de/volltexte/2014/947/pdf/Imran_Thesis.pdf
Jayakannam, M., Bose, J., Babourina, O., Rengel, Z., Shabala, S. 2013. Salicylic acid improves salinity tolerance in Arabidopsis by restoring membrane potential and preventing salt-induced K+ loss via a GORK channel. Journal of Experimental Botany, 64(8), pp. 55-68. Available at https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3654417
Jisha K. C., Vijayakumari K., Puthur J.T. 2013. Seed priming for abiotic stress tolerance: An overview. Acta Physiologia Plantarum, 35(5), pp. 1381–1396. Available at https://link.springer.com/article/10.1007/s11738-012-1186-5
Kasanah, N., Maryani. 2017. Roots anatomy and growth responses of soybean (Glycine max (L.)Merr.) ‘Wilis” to NaCl stress. J. Trop. Biodiv. Biotech. 2, pp. 1-9. Available at https://link.springer.com/chapter/10.1007/978-3-319-60363-6_20
Khan, M.I.R., Fatma, M. Per, T.S., Anjum, N.A., Khan, N.A. 2013. Salicylic acid-induced abiotic stress tolerance and underlying mechanisms in plants. Frontiers in Plant Science, 6, pp. 462-497. Available at https://www.ncbi.nlm.nih.gov/pubmed/26175738
Khodary, S.E.A. 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt stressed maize plants. International Journal of Agriculture and Biology, 6, pp. 5-8. Available at citeseerx.ist.psu.edu/viewdoc/download?doi=10.1.1.322.9285&rep=rep1...
Li, T., Hu, Y., Du, X., Tang, H., Shen, C., Wu, J. 2014. Salicylic acid alleviates the adverse effects of salt stress in Torreya grandis cv. Merrillii seedlings by activating photosynthesis and enhancing antioxidant systems. PLoS ONE, 9(10), pp. e109492. doi: 10.1371/journal.pone.0109492
Mukhtar, K., Afzal, I., Qasim, M., Basra, S.M.A., Shahid, M. 2013. Does priming promote germination and early stand establishment of french marigold (Tagetes patula L.) seeds by inducing physiological and biochemical changes? Acta Scientiarum Polonorum Hortorum Cultu, s 12(3):12-31. Available at http://wydawnictwo.up.lublin.pl/acta/hortorum_cultus/2013/streszczenia2013_3/02%20Mukhtar%20Qasim%20Hort%2012_3_%202013.pdf
Munns, R. & Tester, M. 2008. Mechanisms of salinity tolerance. Annual Review of Plant Biology, 59, pp. 651-681. doi: 10.1146/annurev.arplant.59.032607.092911
Nawaz, J., Hussain, M., Jabbar, A., Nadeem, G.A., Sajid, M., Subtain, M., Shabbir, I. Seed priming a technique. IJACS, 2013, 6(20), pp. 1373-1381. Available at https://pdfs.semanticscholar.org/10c5/260315229e9b27479706ab2d06920d3df0d0.pdf
Nugraheni, I., Solichatun, Anggarwulan, E. 2003. Pertumbuhan dan akumulasi prolin tanaman orok-orok (Crotalaria juncea L.) pada salinitas CaCl2 berbeda. BioSMART, 5(2), pp. 98-101. Available at biosains.mipa.uns.ac.id/B/B0502/B050206.pdf
Palungkun, R. & Asiani, B. 2004. Sweet Corn-Baby Corn: Peluang Bisnis, Pembudidayaan dan Penanganan Pasca Panen. Penebar Swadaya. Jakarta. p.79. Available at digilib.usu.ac.id/.../Sweet-corn-baby-corn-:-peluang-bisnis-pembud...
Pangaribuan, N. 2001. Hardening dalam Upaya Mengatasi Efek Toksik pada Tanaman Bayam (Amaranthus sp.). Jurnal Matematika, Sains dan Teknologi, (1), pp. 25-29.
See comment in PubMed Commons belowPaparella S., Araújo S.S., Rossi G., Wijayasinghe M., Carbonera D., Balestrazzi A. 2015. Seed priming: state of the art and new perspectives. Plant Cell Rep.34(8):1281-1293. doi: 10.1007/s00299-015-1784-y. Available at https://www.ncbi.nlm.nih.gov/pubmed/25812837
Ramayani, Basyuni, M., Agustina, L. 2012. Pengaruh salinitas terhadap pertumbuhan dan biomassa semai dan kandungan lipida pohon non-sekresi Ceriops tagal. Peronema Forestry Science Journal, 1(1), pp. 1-9. Available at https://jurnal.usu.ac.id/index.php/PFSJ/article/view/560
Rehman, H., M. Farooq, S.M.A. Basra and I. Afzal, 2011. Hormonal priming with salicylic acid improves the emergence and early seedling growth in cucumber. J. Agric. Soc. Sci., 7, pp. 109–113. Available at http://www.academia.edu/27169495/Hormonal_Priming_with_Salicylic_Acid_Improves_the_Emergence_and_Early_Seedling_Growth_in_Cucumber
Romero-Aranda, R., Soria, T., Cuartero, J. 2001. Tomato plant-water uptake and plant-water relationship under saline growth conditions. Plant Science, 160, pp. 265-272. Available at https://www.sciencedirect.com/science/article/pii/S0168945200003885
Ruzin SE. 1999. Plant microtechnique and microscopy. Oxford, New York: Oxford University Press
Sedghi, M., Bahman, A.B., Javad, B. 2014. Physiological enhancement of medicinal pumpkin seeds (Cucurbita pepo var. styriaca) with different priming methods. Iranian Journal of Plant Physiology, 5(1), pp. 1209-1215. Available at www.sid.ir/En/Journal/ViewPaper.aspx?ID=518212
Sudaryanto, T., Kustiari, R., Saliem, H.P. 2010. Analisis Sumber Daya Lahan Menuju Ketahanan Pangan Berkelanjutan. BPP Pertanian. Jakarta. pp.1-23.
Suwignyo, R.A., Hayati, R., Mardiyanto. 2010. Toleransi tanaman jagung terhadap salinitas dengan perlakuan stress awal rendah. Jurnal Agrivor, 10(1), pp. 73-83. Available at http://download.portalgaruda.org/article.php?article=29702&val=2165
Wawo, A.H. 2008. Study on seed germination and seedling growth models of sandalwood (Santalum album L.) of several mother seed trees in Belu Regency, East Nusa Tenggara. Biodiversitas, 9(2), pp. 117 - 122. Available at https://smujo.id/biodiv/article/view/393
Xie, Z., Zhang, Z., Hanzlik, S., Cook, E., Shen, Q.J. 2007. Salicylic acid inhibits gibberellin-induced alpha-amylase expression and seed germination via a pathway involving an abscisic-acid-inducible WRKY gene. Plant Molecular Biology, 64 (3), pp. 293–303. Available at https://www.ncbi.nlm.nih.gov/pubmed/17390108
Zadehbagheri, M. 2014. Salicylic acid priming in corn (Zea mays L. var. Sc.704) Reinforces NaCl tolerance at germination and the seedling growth stage. International Journal of Bioscience, 4(5), pp. 187-197. Available at https://www.cabdirect.org/cabdirect/abstract/20143125731
Zahra, S., Amin, B., Ali, V.S., Ali, Y. Mehdi, Y. 2010. The salicylic acid effect on the tomato (Lycopersicum esculentum Mill.) sugar, protein and proline contents under salinity stress. Journal of Biophysics and Structural Biology, 2(3), pp. 35-41. Available at www.academicjournals.org/journal/JBSB/article-full-text-pdf/39E13B910812
DOI: https://doi.org/10.22146/ipas.39621
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