Peran kecambah kacang hijau (Phaseolus radiatus (L.)) pada sel lemak retroperitoneal tikus Sprague Dawley yang diberi diet tinggi lemak

https://doi.org/10.22146/ijcn.41676

Dwi Lestari(1*), Wiryatun Lestariana(2), Lily Arsanti Lestari(3), Arta Farmawati(4)

(1) Universitas Nahdlatul Ulama Nusa Tenggara Barat
(2) Departemen Biokimia, Fakultas Kedokteran, Kesehatan Masyarakat, dan Keperawatan Universitas Gadjah Mada
(3) Departemen Gizi Kesehatan, Fakultas Kedokteran, Kesehatan Masyarakat, dan Keperawatan Universitas Gadjah Mada
(4) Departemen Biokimia, Fakultas Kedokteran, Kesehatan Masyarakat, dan Keperawatan Universitas Gadjah Mada
(*) Corresponding Author

Abstract


The role of mung bean sprouts (Phaseolus radiatus (L.)) on retroperitoneal fat cells of Sprague Dawley rats fed a high-fat diet

Background: The tendency of current lifestyles with high consumption patterns of fat is one of the factors causing obesity which is a risk of cardiovascular disease. Prevention of cardiovascular disease can be done by adopting a dietary pattern that is rich in antioxidants including consumption of sources of vitamin E. Sprouts from mung beans contain vitamin E and phytochemicals rich in antioxidants, so they can be used as an alternative to prevent hyperlipidemia sourced from daily functional food.

Objective: To assess the effects of mung bean sprouts on retroperitoneal fat weight, cell number and cell diameter in rats fed high-fat feed.

Method: This research is an experimental study with a post-test design with the control group. Thirty-four male Sprague Dawley rats ± 2 months old were divided into five groups, group I (standard feed), group II (high-fat feed), group III (high-fat feed and intervention of feeding tube of mung bean sprout 0.67 g/ 200 g BW), group IV (high fat feed and intervention of feeding tube of mung bean sprout 1.34 g / 200 g BW), and group V (high-fat feed and 23 IU dose of vitamin E supplement with feeding tube). After adaptation for three days, the intervention was carried out for four weeks. Analysis of fat weight, fat cell number, and fat cell diameter were done after the treatments. Data analysis using the One-Way ANOVA test.

Results: Mung bean sprout dose of 1.34 g given for 4 weeks did not significantly decrease fat weight and cell number, but it decreased the diameter of retroperitoneal fat cells.

Conclusion: Mung bean sprouts dose 1.34 g was better than a dose of 0.67 g and vitamin E supplementation in reducing the diameter of retroperitoneal fat cells in rats fed a high-fat diet.


Keywords


high-fat diet; mung bean sprouts; retroperitoneal fat cells

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References

  1. WHO. Obesity: preventing and managing the global epidemic. Geneva: WHO; 2008.
  2. Azwar A. Kecenderungan masalah gizi dan tantangan di masa datang. [series online] 2004 [cited Agustus 2014]. Available from: URL: http://www.academia.edu/28601844/kecenderungan_masalah_gizi_dan_tantangan_di_masa_datang
  3. Bose K, Bhadra M, Mukhopadhyay A. Causes and consequences of obesity. Anthropologist Special [series online] 2007 [cited September 2014];3:223-40. Available from: URL: https://pdfs.semanticscholar.org/c593/74afe2e06fd593b0483e09e7e69a6136bdfc.pdf
  4. Wijga AH, Scholtens S, Bemelmans WJ, Gerritsen J, Brunekreef B, Smit HA, et al. Comorbidities of obesity in school children: a cross sectional study in the PIAMA birth cohort. BMC Pub Health 2010;10(1):184. doi: 10.1186/1471-2458-10-184
  5. Riskesdas. Kecenderungan prevalensi obesitas populasi di Indonesia. Jakarta: Riskesdas; 2013.
  6. WHO. Cardiovascular disease (CVDs). [series online] 2017 [cited Jan 2018]. Available from: URL: https://www.who.int/news-room/fact-sheets/detail/cardiovascular-diseases-(cvds)
  7. Kavanagh K, Jones KL, Sawyer J, Kelley K, Carr JJ, Wagner JD, Rudel LL. Trans fat diet induces abdominal obesity and changes in insulin sensitivity in monkeys. Obesity (Silver Spring) 2007 Jul;15(7):1675-84. doi: 10.1038/oby.2007.200
  8. Barzilai N, Gupta G. Revisiting the role of fat mass in the life extension induced by caloric restriction. J Gerontol A Biol Sci Med Sci 1999 Mar;54(3):B89-96. doi: 10.1093/gerona/54.3.b89
  9. Astawan M. Kacang hijau, antioksidan yang membantu kesuburan pria. [series online] 2005 [cited September 2014]. Available from: URL: http://web.ipb.ac.id/~tpg/de/pubde_ntrtnhlth_kacanghijau.php
  10. Shetty K, Lin YT, McCue P, Labbe RG, Randhir R, Ho CY. Low microbial load sprouts with enhanced antioxidants for astronaut diet. SAE Technical Paper 2003-01-2380, 2003. doi: 10.4271/2003-01-2380
  11. Winarsi H. Antioksidan alami & radikal bebas. Yogyakarta: Kanisius; 2007.
  12. Purwandhono A. Pengaruh pemberian ekstrak tauge (Vigna radiata (L)) terhadap kadar kolesterol LDL dan perkembangan aterosklerosis pada tikus wistar jantan hiperkolesterolemi [Skripsi]. Jember: Skripsi Universitas Jember; 2013.
  13. Tita SA. Efek nefroprotektif ekstrak tauge (Vigna radiata (L.) terhadap peningkatan kadar kreatinin serum tikus wistar yang diinduksi parasetamol dosis toksik [Skripsi]. Jember: Skripsi Universitas Jember; 2013.
  14. Iqbal AM. Pengaruh ekstrak tauge (Phaseolus Radiatus) terhadap kerusakan sel ginjal mencit (Mus Musculus) yang diinduksi parasetamol [Skripsi]. Solo: Skripsi Universitas Sebelas Maret; 2010.
  15. Kumar V, Abbas AK, Fausto N, Mitchell R. Robbins basic pathology 8th edition. Philadelphia: WB Saunders Company; 2010.
  16. Kumar A, Sriwastwa VMS, Lata S. Impact of black T supra on haematology of albino rats. Indian J Sci Res 2011;2(4):21-7.
  17. Mitruka BM, Rawnsly HM. Clinical, biochemical, and hematological refference values in normal experimental animals and normal humans 2 ed. USA: Year Book Medical Publishers Inc; 1981.
  18. Rodgers WL, Rozin P. Novel food preferences in thiamine-deficient rats. J Comp Physiol Psychol 1966;61(1):1-4. doi: 10.1037/h0022864
  19. Jacques M. Neurobiology of feeding and nutrition. California: Academic Press Inc; 2012.
  20. Trilling JS, Jaber R. Selections from current literature: the role of free radicals and antioxidants in disease. Fam Pract 1996;13(3):322-6. doi: 10.1093/fampra/13.3.322
  21. Bozaykut P, Karademir B, Yazgan B, Sozen E, Siow RC, Ozer NK, et al. Effects of vitamin E on peroxisome proliferator-activaed receptor γ and nuclear factor-erythroid 2-related factor 2 in hypercholesterolemia-induces atherosclerosis. Free Radic Biol Med. 2014 May;70:174-81. doi: 10.1016/j.freeradbiomed.2014.02.017
  22. Mayes PA. Struktur dan fungsi vitamin larut-lipid. In: Biokimia Harper edisi XXV. Jakarta: EGC; 2003.
  23. Eitenmiller R, Lee J. Vitamin E food chemistry, composition, and analysis. New York: Marcel Decker Inc; 2004.
  24. Murray RK, DK Granner, PA Mayes, VW Rodwell. Biokimia Harper. Jakarta: Penerbit Buku Kedokteran EGC; 2003.
  25. Habibullah LS, Bilbis MJ, Ladan OP, Ajagbona, Saidu. Agueous extract of hibiscus sabdariffa calyces reduces serum trygliserida. Asian Journal of Biochemistry 2007;2(1):42-9. doi: 10.3923/ajb.2007.42.49
  26. Casimir CA, David BM. Food lipids: chemistry, nutrition, and biotechnology second edition. New York: Marcel Dekker AG; 2002.
  27. Cryer A, RLR Van. New perspectives in adipose tissue – structure, function and development. United Kingdom: Butterworth & Co; 2014.
  28. Azain MJ, Dorothy BH, Mattew BS, William PF, Dennis EJ. Dietary conjugated linoleic acid reduces rat adipose tissue cell size rather than cell number. J Nutr 2000;130(6):1548-54. doi: 10.1093/jn/130.6.1548
  29. Hirsch J, Fried SK, Edens NK, Leibel RL. The fat cell. Med Clin North Am 1989;73(1):83-96. doi: 10.1016/s0025-7125(16)30693-9
  30. Spalding KL, Arner E, Westermark PO, Bernard S, Buchholz BA, Arner P, et al. Dynamics of fat cell turnover in humans. Nature 2008 Jun;453(7196):783-7. doi: 10.1038/nature06902
  31. Michaud DS, Giovannucci EL, Ascherio A, Rimm EB, Forman MR, Willett WC, et al. Asociation of plasma carotenoid concentrations and dietary intake of specific carotenoids in samples of two prospective cohort studies using a new carotenoid database. Cancer Epidemiol Biomarkers Prev 1998;7(4):283-90.
  32. Crawford RC, Kirk EA, Rosenfeld ME, LeBoeuf RC, Chait A. Dietary antioxidants inhibit development of fatty streak lesions in the LDL receptor deficient mouse. Arterioscler Thromb Vasc Biol 1998;18(9):1506-13. doi: 10.1161/01.atv.18.9.1506
  33. Burta O, F Tirlea, OL Burta, SM Qadri. Phytotherapy in cardiovascular diseases: from ethnomedicine to evidence based medicine. J Biol Sci 2008;8(2):242-7.
  34. Stiphanuk MH. Biochemical and physiological aspects of human nutrition. New York: Saunders; 2000
  35. Dawn BM, Allan DM, Smith CM. Biokimia kedokteran dasar: sebuah pendekatan klinis. Jakarta: EGC; 2000.
  36. Worhingham RBG, William SR. Nutrition throughout the life cycle. Washington: Mosby; 1996.
  37. Steinberg MF, Chalt A. Antioxidant vitamin supplementation and lipid peroxidation in smokers. Am J Clin Nutr 1998 Aug;68(2):319-27. doi: 10.1093/ajcn/68.2.319
  38. Corrales MVF, Musher DM, Wells GA, Chirinos JA, Chen L, Fine MJ. Cardiac complications in patients with community-acquired pneumonia incidence, risk factors, and association with short-term mortality. Circulation 2012;125(6):773-81. doi: 10.1161/CIRCULATIONAHA.111.040766
  39. Lamson DW, Brignall MS. Antioxidants and cancer III: quercetin. Altern Med Rev 2000;5(3):196-208.



DOI: https://doi.org/10.22146/ijcn.41676

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