Pencegahan hipertensi dan penebalan dinding aorta dengan pemberian kecambah kacang hijau (Phaseolus radiatus (L)) pada tikus putih Sprague Dawley

Novian Swasono Hadi; Arta Farmawati; Ahmad Ghozali

doi:10.22146/ijcn.22454

Pencegahan hipertensi dan penebalan dinding aorta dengan pemberian kecambah kacang hijau (Phaseolus radiatus (L)) pada tikus putih Sprague Dawley

https://doi.org/10.22146/ijcn.22454

Novian Swasono Hadi^(1*), Arta Farmawati⁽²⁾, Ahmad Ghozali⁽³⁾

(1) Jurusan Gizi Politeknik Kesehatan Kementerian Kesehatan Gorontalo
(2) Departemen Biokimia, Fakultas Kedokteran Universitas Gadjah Mada
(3) Departemen Patologi Anatomi, Fakultas Kedokteran Universitas Gadjah Mada
(*) Corresponding Author

Abstract

Background: Lifestyle changes with high-fat food consumption is one of the factors the risks of cardiovascular diseases like of coronary heart disease and atherosclerosis. A healthy diet and a balanced diet and consume foods that contain lots of antioxidants is one of the effective ways to prevent hyperlipidemia. Mung bean sprouts have properties that neutralize free radicals cause Hyperlipidemia and cardiovascular diseases because it is an antioxidant compound.

Objective: The aim of this study was to determinate the effect of mung bean sprouts (Phaseolus radiatus (L)) to blood pressure and histopathology aorta of Sprague-Dawley male rats.

Method: The type of study was experimental research using pre-post test controlled group design for blood pressure variable and post test only controlled group design histopathology aorta. The thirty-five of Sprague-Dawley male rats was eight weeks divided into 5 groups. The first group was given standard diet, group 2 was given a hight fat diet, the third group was given a high-fat diet and mung bean sprout 0,67 gram, group 4 was given a high-fat diet and mung bean 1,34 gram, and group 5 was given a high-fat diet and vitamin E doses of 23 IU.

Results: Result of this study showed that after 4 weeks of treatment, increased in blood pressure systole in the given of high fat diet higher than group who were given a high fat diet and mung bean sprout and also on group who were given high fat diet and vitamin E, but there is no difference effect a decrease in blood pressure between the provision of mung bean sprouts and vitamin E (p>0,05). Statistical analysis to thick the wall the aorta show the similarity meaningful in all the treatment group, it can be said that overall thick the wall the aorta in this research is not different.

Conclusion: A dose of mung bean sprout 0,67 g is optimal doses in preventing a rise in blood pressure and prevent alterations histopathology Sprague-Dawley male rats.

Keywords

blood pressure; high fat diet; histopatology aorta; mung bean sprouts

Full Text:

PDF

References

Depkes RI. Lingkungan sehat jantung sehat. [series online] 2014 [cited 2015 January 18]. Available from: URL: http://www.depkes.go.id/lingkungan-sehat-jantung-sehat%20(depkes).html
WHO. Cardiovascular disease (CVDs). [series online] 2010 [cited 2015 January 18]. Available from: URL: http://www.who.int/mediacebtre/factsheets/fs317/
Wijaya A. Parameter biokimiawi untuk sindrom koroner akut (dalam) forum diagnisticum. Prodia Educational Services; 2000.
Price SA, Wilson LM. Patofisiologi, konsep klinis proses-proses penyakit. Alih Bahasa: Pendit BU, Hartanto H, Wulansari P, Mahanani DA. Jakarta: EGC; 2006.
Lautan J. Radikal bebas pada eritrosit dan leukosit. Cermin Dunia Kedokteran 1997;116-130.
Anggrahini S. Pengaruh lama pengecambahan terhadap kandungan α-tokoferol dan senyawa proksimat kecambah kacang hijau (Phaseolus radiatus L.). [Thesis]. Yogyakarta: Fakultas Pertanian UGM; 2009.
Purwono, Hartono R. Kacang hijau. Jakarta: Penebar Swadaya; 2005.
Daud R. Efek suplementasi vitamin E terhadap perubahan histopatologis jantung mencit yang diberi ransum lemak tinggi. J Ked Hewan Univ Syah Kuala 2007;1(1).
National Institute of Health. Vitamin E. Health Professional. [series online] 2010 [cited February 05, 2015]. Available from: URL: http://ods.od.nih.gov/factsheets/VitaminE-HealthProfessional/
Blessing IA, Gregory IO. Effect of processing on the proximate composition of the dehulled and undehulled mungbean (Vigna radiate (L.)) Wilczek) flours. Pak J Nutr 2010;9(10):1006-16.
Adam LB. Guidelines for adolescent nutrition service hyperlipidemia center of leadership, education, and training and child nutrition. Division of Epydemiology and Community Health, School of Public Health. US: University of Minnesota; 2006.
Mayes PH. Pengangkutan dan penyimpanan lemak. Jakarta: EGC; 2003.
Guyton AC, Hall JE. Textbook of medical physiology, 11^th. Philadelphia: WB Sounders Company; 2006.
Bohr DF, McIntyre M, Dominiczak AE. Endothelial Function in hypertension; the role of superoxida anion. Hypertension 1999;34:539-45.
Malonda NSH, Dinarti LK, Pangastuti R. Pola makan dan konsumsi alkohol sebagai faktor risiko hipertensi pada lansia. Jurnal Gizi Klinik Indonesia 2012;8(4):202-12.
Taniyama Y, Griendling K. Reactive oxygen species in the vasculature. Molecular and cellular mechanisms. J Hypertension 2003;42:1075-81.
Tambunan S, Asni E, Malik Z. Histopatologi aorta torasika tikus putih (Rattus norvegicus strain Wistar) jantan setelah pemberian diet aterogenik selama 12 minggu. Jurnal Online Mahasiswa (JOM) Bidang Kedokteran 2015;2(1):1-14.
Schafer FQ, Kelley EE, Buettner GR. Oxidative stress and antioxidant intervention in critical reviews of oxidative stress and aging: advances in basic science, diagnostics and intervention. Ed: Cutler R, Rodriguez H. New Jersey: World Scientific; 2003.
Astawan M. Kacang hijau, antioksidan yang membantu kesuburan pria. Bogor: Departemen Teknologi Pangan Dan Gizi IPB; 2005.
Maslachah L, Sugihartuti R, Kurniasanti R. Hambatan produksi reactive oxygen species radikal superoksida oleh antioksidan vitamin e pada tikus putih yang menerima stressor ranjatan listrik. Media Kedokteran Hewan 2008;24(1):21-6.