Pengaruh yogurt sinbiotik pisang terhadap kadar glukosa dan insulin tikus sindrom metabolik

Fiqhi Cahyaningrum Rahmawati(1*), Kis Djamiatun(2), Nyoman Suci(3)

(1) Program Studi Magister Ilmu Gizi, Fakultas Kedokteran Universitas Diponegoro
(2) Bagian Biomedik, Fakultas Kedokteran Universitas Diponegoro
(3) Bagian Patologi Klinik, Fakultas Kedokteran Universitas Diponegoro / Rumah Sakit Umum Pusat Dr. Karyadi
(*) Corresponding Author


Background: The metabolic syndrome often begins with insulin resistance characterized by hyperinsulinemia and hyperglycemia. Synbiotic yoghurt tanduk banana contains probiotic and prebiotic FOS can improve insulin resistance in metabolic syndrome.

Objective: To prove the effect of synbiotic yoghurt tanduk banana can reduce blood glucose levels and insulin levels in metabolic syndrome rats.

Method: True-experimental study with randomized controlled group pre-post test design in male wistar rats. Eighteen samples were induced to become metabolic syndrome with high fat fructose diet for 2 weeks and they were divided into 3 groups: control (K) was given standard diet, treatment group 1 (P1), and treatment group 2 (P2) were given sinbiotic yoghurt tanduk banana dose of 0.009 ml/g body weight (BW)/day and 0.018 ml/g BW/day for 2 weeks.

Results: The paired t-test showed there were differences of blood glucose levels and insulin levels before and after treatment in P2 group. The Kruskal Wallis test showed there were differences of mean blood glucose levels inter group after treatment (p<0.05). The One-way Anova test showed there were differences of mean insulin levels inter group after treatment (p<0.05).

Conclusion: Synbiotic yoghurt tanduk banana can reduce blood glucose levels and insulin levels in metabolic syndrome rat with effective dose is 0.018 ml/grBW/day.


blood glucose; insulin; metabolic syndrome; synbiotic yoghurt banana

Full Text:



  1. National Cholesterol Education Program (NCEP) Expert Panel on Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults (Adult Treatment Panel III). Third report of the national cholesterol education program (NCEP) expert panel on detection, evaluation, and treatment of high blood cholesterol in adults (adult treatment panel III) final report. Circulation. 2002;106:3143-421.
  2. International Diabetes Federation. The IDF consensus worldwide definition of the metabolic syndrome. [series online] 2006 [cited 2017 Januari]. Available from: URL:
  3. Nelms M, Sucher K, Lacey K, Roth S. Nutrition therapy and phatophysiology. In: Cosio Y, ed. Cengage Learning. Inc. Second edition. California, USA: Brooks/Cole Cengage Lerning; 2010.
  4. Yin Q, Chen X, Li L, Zhou R, Huang J, Yang D. Apolipoprotein B/apolipoprotein A1 ratio is a good predictive marker of metabolic syndrome and pre-metabolic syndrome in Chinese adolescent women with polycystic ovary syndrome. J Obstet Gynaecol Res. 2013;39(1):203-9.
  5. Rolfes S, Pinna K, Ellie W. Diabetes melitus. In: Understanding normal and clinical nutrition. Eighth. California, USA: Brooks/Cole Cengage Lerning; 2009.
  6. Shaghaghi M, Pourahmad R, Adeli H. Synbiotic yogurt production by using prebiotic compounds and probiotic lactobacilli. Intl Res J Appl Basic Sci. 2013;5(7):839-46.
  7. Nikjooy S, Hashemi S. Study the possibility of producing symbiotic yogurt containing lactobacillus casei and wild thyme extract. Intl J Agri Crop Sci. 2015;8(1):61-7.
  8. Standar Nasional Indonesia. Yogurt. ICS 67.100.10. SNI 2981; 2009.
  9. Jenie B, Widowati S, Nurjanah S. Pengembangan produk tepung pisang dengan indeks glikemik rendah dan sifat prebiotik sebagai bahan pangan fungsional. Jurnal Teknologi dan Ilmu Pangan Institut Pertanian Bogor. 2009;1-31.
  10. Karlina R, Rahayuni A. Potensi yoghurt tanpa lemak dengan penambahan tepung pisang dan tepung gembili sebagai alternatif menurunkan kolesterol. Journal of Nutrition College. 2013;3(2):16-25.
  11. Nadimin, Sri D, Sadariah. Pengaruh pemberian diit tinggi serat terhadap penurunan kadar gula darah pasien DM tipe-II di RSUD Salewang Kab. Maros. Media Gizi Pangan. 2009;VII(1):1-5.
  12. Moroti C, Magri L, Costa M, Cavallini D, Sivier K. Effect of the consumption of a new symbiotic shake on glycemia and cholesterol levels in elderly people with type 2 diabetes mellitus. Lipids Health Dis. 2012;11:29.
  13. Laitinen K, Poussa T, Isolauri E. Nutrition, Allergy, mucosal immunology and intestinal microbiota group. probiotics and dietary counselling contribute to glucose regulation during and after pregnancy: a randomised controlled trial. Br J Nutr. 2009;101(11):1679-87.
  14. Slavin J. Fiber and prebiotics: mechanisms and health benefits. Nutrients. 2013;5:1417-35.
  15. Besten G, Eunen K, Groen A, Venema K, Reijngoud D, Bakker B. The role of short-chain fatty acids in the interplay between diet, gut microbiota, and host energy metabolism. J Lipid Res. 2013;54:2325-40.
  16. Octavia ZF, Djamiatun K, Suci N. Pengaruh pemberian yogurt sinbiotik tepung pisang tanduk terhadap profil lipid tikus sindrom metabolik. Jurnal Gizi Klinik Indonesia. 2017;13(4):159-69.
  17. World Health Organization (WHO). General guidelines for metodologies on research and evaluation of traditional medicine. Geneva: WHO; 2001.
  18. Muflikhatur S, Murwani H. Perbedaan pengaruh antara ekstrak dan rebusan daun salam (Eugenia polyantha) dalam pencegahan peningkatan kadar kolesterol total pada tikus Sprague Dawley. Journal of Nutrition College. 2014;3(1):142-9.
  19. Ratnawati H, Widowati W. Anticholesterol activity of velvet bean (Mucuna Pruriens L.) towards hypercholesterolemic rats. Sains Malaysiana. 2011;40(4):317-21.
  20. Zhang M, Lv X, Li J, Xu Z, Chen L. The characterization of high-fat diet and multiple low-dose streptozotocin induced type 2 diabetes rat model. Exp Diabetes Res. 2008;23:1-9.
  21. Crescenzo R, Bianco F, Coppola P, Mazzoli A, Tussellino M, Carotenuto R, et al. Fructose supplementation worsens the deleterious effects of short-term high-fat feeding on hepatic steatosis and lipid metabolism in adult rats. Exp Physiol. 2014;99(9):1203-13.
  22. Warditiani N. Uji aktivitas antihiperlipidemia dan antiaterosklerosis isolat andrografolid dan ekstrak terpurifikasi herba sambiloto (Andrographis paniculata (Burm.f) Ness) pada tikus diabetes mellitus tipe 2 resistensi insulin [Tesis].Yogyakarta: Fakultas Farmasi Universitas Gadjah Mada; 2012.
  23. Bender D. Energy nutrition-the metabolism of carbohydrates and fats. In: Bender DA, editor. Introduction to nutrition and metabolism. Fourth ed. Boca Raton: CRC Press; 2008.
  24. Yadav H, Jain S, Sinha P. Antidiabetic effect of probiotic dahi containing Lactobacillus acidophilus and Lactobacillus casei in high fructose fed rats. Nutrition. 2007;23(1):62-8.
  25. Hajiaghaalipour F, Khalilpourfarshbafi M, Arya A. Modulation of glucose transporter protein by dietary flavonoids in type 2 diabetes mellitus. Int J Biol Sci. 2015;11(5):508-24.
  26. Widowati W. Potensi antioksidan sebagai antidiabetes. JKM. 2008;7(2):1-11.
  27. Sinatra S, Simpson G. “Fire in the heart”: new developments in diagnosis, prevention & treatment of cardiovascular disease. Chapter 4. [series online] 2004 [cited 2017 Januari]. Available from: URL:
  28. Muniyappa R, Lee S, Chen H, Quon MJ. Current approaches for assessing insulin sensitivity and resistance in vivo: advantages, limitations, and appropriate usage. Am J Physiol Endocrinol Metab. 2008;294(1):E15-26.
  29. Aronoff S, Berkowitz K, Shreiner B, Want L. Glucose metabolism and regulation: beyond insulin and glucagon. Diabetes Spectrum. 2004;17(3):183-90.


Article Metrics

Abstract views : 5395 | views : 8262


  • There are currently no refbacks.

Copyright (c) 2017 Jurnal Gizi Klinik Indonesia

Creative Commons License
This work is licensed under a Creative Commons Attribution-ShareAlike 4.0 International License.

Jurnal Gizi Klinik Indonesia (JGKI) Indexed by:

web stats View My Stats