Kontribusi peroksidasi lipid terhadap kerusakan sel hati tikus putih akibat keracunan Aflatoksin B1

Abstract

The experiment used 96 white rats (Rattus norvegicus) with age around eight weeks old and weight 180-200
grams, divided into four groups of 24 rats each, based on the dosages ofAflatoxin B1 given. Each group was
divided further into three subgroups of eight rats based on the length of exposure time ofAflatoxin1.
Four dosages ofAflatoxin1 were administered orallyeveryday into different groups, consisted of 0 μg, 10 μg,
15 μg, and 20 μg, dissolved in 0.2ml propylene glycol. Three subgroups received the dosage for 12 weeks, 16
weeks, and 20 weeks.At the end of the experiment, the rats were sacrificed,malondialdehyde were analyzed
using Uchiyama and Mihara method. Liver cell damages were examined using histological slices stained by
haematoxilin eosin. Data was analyzed using analysis of variance, and P<0.05 was considered to be significantly
different.
Data analysis shows that : 1) there were significant differences between the effects of 12 weeks and 20
weeks exposure, and between dosage of 10 μg and 20 μg on increasingmalondialdehyde of liver tissue and
liver cell damage. This also showed that increasing exposure time and dosages of Aflatoxin1 increasing in
malondialdehyde of liver tissue and liver cell damage. 2) There were no significant differences between dosages
10 μg and 15 μg, between 15 μg and 20 μg, between 12 weeks and 16 weeks, and between 16 and 20
weeks exposures, on increasingmalondialdehyde of the liver tissue. 3) themoreAflatoxin1 was given themore
liver cells damage. It is concluded that aflatoxin1may cause liver cell damage as the result of liver peroxidation
and many other factors as well.