GENETIC VARIATION AMONG LOCAL SHEEP IN INDONESIA USING MICROSATELLITE DNA

Abstract

The objectives of this study were to evaluate the genetic variation and examine the
genetic distance of Indonesian local sheep among east part and west part using CSSM018; ILST054 and IDVGA30 microsatellite loci. Data were collected from eight sheep populations, four populations from East part (Rote, Sumbawa and Donggala) and five populations from west part (Ciomas, Jonggol Margawati Madura and Indramayu). Genetic variation for each sheep population were calculated in the form of allelic (total number alleles, mean number of alleles per locus, frequency of every allele in each population) according to Nei (1987): The phylogenetic trees were constructed using UPGMA (unweighted pair-group method with arithmetic mean) based on the genetic distance (D). The result showed that CSSM0l8; ILST054 and lDVGA30 microsatellite loci. Exhibited a total of l3 alleles from eight populations. Allele cl in locus CSSM0l8 has only found in Madura sheep. Allele b in locus loci ILST 054 was found only in Margawati (00833) and allele d was found only in J onggol (00833) and in lndramayu (0.0455). The heterozygosity value (h) of loci CSSM O18 was high (0.5680) followed by IDVGA-30 (05190) and ILST054 (0.3486) and statistically different t = 6.75, p<0.05. The Indramayu population has the highest average heterozygosity (El) value (0.5426) followed by Margawati (0.5372),
Madura (04887), Jonggol (04758), Sumbawa (03309), Ciomas (03105), and Donggala (02935) and Rote (O.ll63). The population which genetic distance was nearest to Margawati was Indramayu (0.0028) followed by Sumbawa (00528), Madura (0.0747), Rote (00848), Ciomas (0.0854), Jonggol (0.1 166) and Donggala (0.l401). Population was furthest to Rote was Jongol (0.2855), and Ciomas (0.2540).